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The map label for this gene is bioB [H]

Identifier: 126696538

GI number: 126696538

Start: 1014839

End: 1015783

Strand: Direct

Name: bioB [H]

Synonym: P9301_12001

Alternate gene names: 126696538

Gene position: 1014839-1015783 (Clockwise)

Preceding gene: 126696537

Following gene: 126696539

Centisome position: 61.81

GC content: 35.66

Gene sequence:

>945_bases
TTGCAAATACTTAATATGCCTCTTATTGATTTAATGTGGGAAGCACAAATCGTTCACAGGAAATTTAACAAATACGATAT
TCAATTAGCATCATTGTTCAGCGTAAAAACTGGTGGATGTGAGGAAAATTGTTCGTACTGTAGCCAATCGATTTATAGTG
CTAGCGAAATCAAAAGTCATCCACAGTTTCAAGTTGAAGAAGTTTTAGCAAGAGCTAAAGTAGCAAAAAATGAGGGTGCA
GATAGGTTTTGTATGGGTTGGGCGTGGAGAGAAATTAGAGATGGGAAATCTTTTAATGCAATGTTGGAGATGGTTAGCGG
TGTAAGGGATTTAGGGATGGAAGCATGCGTTACTGCTGGGATGCTTACAGAAGAACAAGCTTCCAGATTGGCTGATGCAG
GTTTGACCGCCTATAACCACAATCTTGATACTAGTCCTGAGCATTATAAAAATATTATTACCACTAGAACTTATCAAGAC
AGACTAGACACTATTAAAAGAGTAAGGAATGCAGGAATAAATGTTTGTTGTGGAGGAATAATAGGCTTGGGTGAAACTAA
TGGCGATAGAGCATCTCTTTTAGAAGTGCTTTCAAACATGAATCCACACCCTGAAAGTGTTCCTATAAATTCATTAGTAG
CTATTGAAGGCACTGGTTTAGAAGATACTCAAGAAATTGATTCTATTGAAATGATAAGGATGATAGCTACAGCAAGAATT
CTTATGCCTGAAAGTAAAATAAGACTAAGTGCAGGGAGAGAAAAGCTTACAAAAGAAGCCCAAATATTATGTTTTCAATG
TGGGGCAAATTCAATTTTTTATGGAGATGAGTTACTCACAACTTCAAATCCATCTTTTCAATCAGACAGAAAACTTCTTA
AAGAGGTTGGAGTATCATTTAACAAAGATTTTGAAACTTGTGAAAAAACATTATCTTCTTTATGA

Upstream 100 bases:

>100_bases
AATCTTTTGCAGATTCTCAATATATTTCCTAATAAAAATGGCTAATTCGAATAATCAGTTATTAAAAGAAATTAGGTACG
ATTGGAATAGGGAGGAGATA

Downstream 100 bases:

>100_bases
AAGGCAAAAATTATAAAATAGTTTCTCTTTACTCTTTCTTCCCATTTCAAGAAAACTTAATTCTTGATCTAAAAAATAAA
TTAATAGAAATCGAAAATGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MQILNMPLIDLMWEAQIVHRKFNKYDIQLASLFSVKTGGCEENCSYCSQSIYSASEIKSHPQFQVEEVLARAKVAKNEGA
DRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQD
RLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTGLEDTQEIDSIEMIRMIATARI
LMPESKIRLSAGREKLTKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVSFNKDFETCEKTLSSL

Sequences:

>Translated_314_residues
MQILNMPLIDLMWEAQIVHRKFNKYDIQLASLFSVKTGGCEENCSYCSQSIYSASEIKSHPQFQVEEVLARAKVAKNEGA
DRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQD
RLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTGLEDTQEIDSIEMIRMIATARI
LMPESKIRLSAGREKLTKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVSFNKDFETCEKTLSSL
>Mature_314_residues
MQILNMPLIDLMWEAQIVHRKFNKYDIQLASLFSVKTGGCEENCSYCSQSIYSASEIKSHPQFQVEEVLARAKVAKNEGA
DRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQD
RLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTGLEDTQEIDSIEMIRMIATARI
LMPESKIRLSAGREKLTKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVSFNKDFETCEKTLSSL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=299, Percent_Identity=52.1739130434783, Blast_Score=330, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6321725, Length=312, Percent_Identity=46.1538461538462, Blast_Score=298, Evalue=8e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 34943; Mature: 34943

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILNMPLIDLMWEAQIVHRKFNKYDIQLASLFSVKTGGCEENCSYCSQSIYSASEIKSH
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
PQFQVEEVLARAKVAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAG
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHH
MLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGI
HHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCE
IGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTGLEDTQEIDSIEMIRMIATARI
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LMPESKIRLSAGREKLTKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVSF
HCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEECCEEEECCCCCHHHHHHHHHHHCCCC
NKDFETCEKTLSSL
CCCHHHHHHHHHCC
>Mature Secondary Structure
MQILNMPLIDLMWEAQIVHRKFNKYDIQLASLFSVKTGGCEENCSYCSQSIYSASEIKSH
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
PQFQVEEVLARAKVAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAG
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHH
MLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGI
HHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCE
IGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTGLEDTQEIDSIEMIRMIATARI
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LMPESKIRLSAGREKLTKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVSF
HCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEECCEEEECCCCCHHHHHHHHHHHCCCC
NKDFETCEKTLSSL
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA