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The map label for this gene is pmbA [H]

Identifier: 126696364

GI number: 126696364

Start: 885597

End: 886949

Strand: Direct

Name: pmbA [H]

Synonym: P9301_10261

Alternate gene names: 126696364

Gene position: 885597-886949 (Clockwise)

Preceding gene: 126696363

Following gene: 126696365

Centisome position: 53.94

GC content: 32.3

Gene sequence:

>1353_bases
ATGAATTCAAAAGAAATAACAACTCAAATCTCTGAAGCTGCAGATTCACTAAATCTTAAAAAATGGGATTATGGTGCAAG
CTTTTCTAATGATTATTCTGTGCAAGTAGATAAAGGTGAGGCTAAACAACTTAAGGCATCACAAAAGCAAATTTTAACTA
TAAGAGTATGGAATAAATCTAATTTAGTTGGTATTACAACAACTAGTGATATCAGTGAATCTGGCATTAAAAAAGCTCTA
AATCAAGCAAATATTGCATCTGATTATGGCAACAAGAATGAAAGAACAGAATTCTCACCACTAGCCAAGGATCCTATTCA
TGTTAAGGACTCAAAAAAAAGAAATCCTGTTGGAATAAAAAAATTACTTATGCTTTTAAGAGAAGCAGAAATAAAACTAT
TAGAAAGCCATGAATCCATAAAATCTGTTCCGTATAATGGTTTATCTGAGAGTTTTTATGAGAGAGTTTATGCAAATAGT
GATGGTGCCTTTAGGAGTTATACCAAAAGCCAAGCTGCACTTTATTTATATGCAAGAGCAGAAGAGAAAAATAAGAAACC
TCGTAGCTCAGGTTCTGTAAAACTTGGATACGGAGTTGAAGATATAGATATAGAGTCGTGTATTAAAGACGCTTCTAATA
AAACAATTTCACATTTAAATTATTCATCTATTAAAACTGATAAATATTTAATATGTTTTTCCCCAGAGTCTTTTTTAACG
ATCATTAATGCCTTTAGTTCAATGTTTAATGCTAGAAGCATTTTAGATGGAGTTAGCTTATCTAATAAAAATTCTATTGG
AGAGAAATTATCTACAGACGCCCTTAATATTTATGATGATGGTCTTCACGAAAAAAATATTTCTTCATTACCGTTTGATG
GAGAGGGAACTCCTACCAAAAGGCTATGTTTAATTAACAAAGGGAGACTTGAAAATTTTATACATTCTGAATCAACAGCA
AGAATATTTAAAACAAGCCCTACAGGTCACGCTGGACTAGGATCAAAAGTCTCAGTATCTCCTGATTGGATAGTAGTTGA
GAAATCAGAGGAAAACTTAGATCTAAAAACATCATTAGATCACTCTACTTATGAGGGAGAATTTGTTTATATTGAAGAAT
TAAATGCAATCCATGCGGGTGTCAGAGCAAGTCAAGGTTCATTCTCTCTTCCATTTGATGGATGGCTCTATAAAAACGGT
AAAAAAATCTCAATAGAATCTGCAACAGTAGCAGGGGATATCAAATATCTTTTGAAAAATATAGTAAATATTGAATCAAA
CCAGGAAGTAACAACAAGTGGAATTTCTCCACATATATGGGTGGATGAATTATCAATAACTGGTGACGCGTGA

Upstream 100 bases:

>100_bases
AGCTCCTGGATTTTGTGGATCCGTCAGTGGAAGTGTCAACGTAACTGTTGGACAACCTCATATCAAGGTTGATTCAATCA
CTGTTGGCGGAAGATAGGAT

Downstream 100 bases:

>100_bases
GAATTATATTCTGGGGAACACCTGAATATTCAATTGCGAGCCTTGATATTTTTATTAAATCTAAGCACGAGGTAATTGGA
GTAGTTAGCCAACCCGATAA

Product: putative modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNSKEITTQISEAADSLNLKKWDYGASFSNDYSVQVDKGEAKQLKASQKQILTIRVWNKSNLVGITTTSDISESGIKKAL
NQANIASDYGNKNERTEFSPLAKDPIHVKDSKKRNPVGIKKLLMLLREAEIKLLESHESIKSVPYNGLSESFYERVYANS
DGAFRSYTKSQAALYLYARAEEKNKKPRSSGSVKLGYGVEDIDIESCIKDASNKTISHLNYSSIKTDKYLICFSPESFLT
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTDALNIYDDGLHEKNISSLPFDGEGTPTKRLCLINKGRLENFIHSESTA
RIFKTSPTGHAGLGSKVSVSPDWIVVEKSEENLDLKTSLDHSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNG
KKISIESATVAGDIKYLLKNIVNIESNQEVTTSGISPHIWVDELSITGDA

Sequences:

>Translated_450_residues
MNSKEITTQISEAADSLNLKKWDYGASFSNDYSVQVDKGEAKQLKASQKQILTIRVWNKSNLVGITTTSDISESGIKKAL
NQANIASDYGNKNERTEFSPLAKDPIHVKDSKKRNPVGIKKLLMLLREAEIKLLESHESIKSVPYNGLSESFYERVYANS
DGAFRSYTKSQAALYLYARAEEKNKKPRSSGSVKLGYGVEDIDIESCIKDASNKTISHLNYSSIKTDKYLICFSPESFLT
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTDALNIYDDGLHEKNISSLPFDGEGTPTKRLCLINKGRLENFIHSESTA
RIFKTSPTGHAGLGSKVSVSPDWIVVEKSEENLDLKTSLDHSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNG
KKISIESATVAGDIKYLLKNIVNIESNQEVTTSGISPHIWVDELSITGDA
>Mature_450_residues
MNSKEITTQISEAADSLNLKKWDYGASFSNDYSVQVDKGEAKQLKASQKQILTIRVWNKSNLVGITTTSDISESGIKKAL
NQANIASDYGNKNERTEFSPLAKDPIHVKDSKKRNPVGIKKLLMLLREAEIKLLESHESIKSVPYNGLSESFYERVYANS
DGAFRSYTKSQAALYLYARAEEKNKKPRSSGSVKLGYGVEDIDIESCIKDASNKTISHLNYSSIKTDKYLICFSPESFLT
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTDALNIYDDGLHEKNISSLPFDGEGTPTKRLCLINKGRLENFIHSESTA
RIFKTSPTGHAGLGSKVSVSPDWIVVEKSEENLDLKTSLDHSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNG
KKISIESATVAGDIKYLLKNIVNIESNQEVTTSGISPHIWVDELSITGDA

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=425, Percent_Identity=22.1176470588235, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49746; Mature: 49746

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKEITTQISEAADSLNLKKWDYGASFSNDYSVQVDKGEAKQLKASQKQILTIRVWNKS
CCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCHHHHHHCCCCEEEEEEEECCC
NLVGITTTSDISESGIKKALNQANIASDYGNKNERTEFSPLAKDPIHVKDSKKRNPVGIK
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHH
KLLMLLREAEIKLLESHESIKSVPYNGLSESFYERVYANSDGAFRSYTKSQAALYLYARA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEEC
EEKNKKPRSSGSVKLGYGVEDIDIESCIKDASNKTISHLNYSSIKTDKYLICFSPESFLT
HHCCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCEEHHCCCCCCCCCCEEEEECCHHHHH
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTDALNIYDDGLHEKNISSLPFDGEGTPTK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCE
RLCLINKGRLENFIHSESTARIFKTSPTGHAGLGSKVSVSPDWIVVEKSEENLDLKTSLD
EEEEEECCHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCEEEEECCCCCCCEEECCC
HSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNGKKISIESATVAGDIKYLLKN
CCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHH
IVNIESNQEVTTSGISPHIWVDELSITGDA
HHCCCCCCEEEECCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MNSKEITTQISEAADSLNLKKWDYGASFSNDYSVQVDKGEAKQLKASQKQILTIRVWNKS
CCCHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCHHHHHHCCCCEEEEEEEECCC
NLVGITTTSDISESGIKKALNQANIASDYGNKNERTEFSPLAKDPIHVKDSKKRNPVGIK
CEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHH
KLLMLLREAEIKLLESHESIKSVPYNGLSESFYERVYANSDGAFRSYTKSQAALYLYARA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEEC
EEKNKKPRSSGSVKLGYGVEDIDIESCIKDASNKTISHLNYSSIKTDKYLICFSPESFLT
HHCCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCEEHHCCCCCCCCCCEEEEECCHHHHH
IINAFSSMFNARSILDGVSLSNKNSIGEKLSTDALNIYDDGLHEKNISSLPFDGEGTPTK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCE
RLCLINKGRLENFIHSESTARIFKTSPTGHAGLGSKVSVSPDWIVVEKSEENLDLKTSLD
EEEEEECCHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCEEEEECCCCCCCEEECCC
HSTYEGEFVYIEELNAIHAGVRASQGSFSLPFDGWLYKNGKKISIESATVAGDIKYLLKN
CCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHH
IVNIESNQEVTTSGISPHIWVDELSITGDA
HHCCCCCCEEEECCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]