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The map label for this gene is cspR [H]

Identifier: 126696336

GI number: 126696336

Start: 868731

End: 869213

Strand: Direct

Name: cspR [H]

Synonym: P9301_09981

Alternate gene names: 126696336

Gene position: 868731-869213 (Clockwise)

Preceding gene: 126696335

Following gene: 126696337

Centisome position: 52.91

GC content: 30.23

Gene sequence:

>483_bases
TTGGAAGTCGCTCTTTTTGAACCTAGAATACCACAAAATACTGGTAATATTGCCAGATCATGTGCTGCGTTTGATATACC
TTTAAATCTTATAGAGCCCCTGGGCTTTAAACTAGAAGATAAATATTTAAAAAGAGCAGGATTAGACTATTGGCCTTTAG
TTACTGTTAATAGGTATGAGAATTTTGAAAAATTTATGACGGCAAAAACAACAAAAAGAATAATTTCTTTTAGTAAAAAA
AATGGATTATATTTGAAGGATTTTAAATTTAAGCAAGATGATATTTTGCTATTTGGGAGAGAAGATTCAGGATTACCCGA
TTCAATTATTGATAAAAGCGACTTTTTAATATCAATATTTATGCCGAATATACAGACTGGAAACAATGATCAAAAAGGTG
TTAGAAGTCTAAACCTTTCTGTAGCATGCGGAATTGCTATATATGAGGCCCACAAACAAATAAATTTTCAAAATGGTAAT
TAA

Upstream 100 bases:

>100_bases
TTCAAAAGAGAATAACCAAAATATCAAAAGATAATTGGTTGGCAATAAATGGTAGAGCAATCAAAATAGATGAAATAAGT
ATTGAAATACCTACTTAAAA

Downstream 100 bases:

>100_bases
GTACAATCAATGCTTTACAATATTCCCATGTCTCGGTAGCTCAGCTGGTTAGAGCGGCGGATTCATAGCCCGCAGGTCGC
GTGTTCAAGTCACGCTCGAG

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MEVALFEPRIPQNTGNIARSCAAFDIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNRYENFEKFMTAKTTKRIISFSKK
NGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIFMPNIQTGNNDQKGVRSLNLSVACGIAIYEAHKQINFQNGN

Sequences:

>Translated_160_residues
MEVALFEPRIPQNTGNIARSCAAFDIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNRYENFEKFMTAKTTKRIISFSKK
NGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIFMPNIQTGNNDQKGVRSLNLSVACGIAIYEAHKQINFQNGN
>Mature_160_residues
MEVALFEPRIPQNTGNIARSCAAFDIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNRYENFEKFMTAKTTKRIISFSKK
NGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIFMPNIQTGNNDQKGVRSLNLSVACGIAIYEAHKQINFQNGN

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=36.5384615384615, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18246; Mature: 18246

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVALFEPRIPQNTGNIARSCAAFDIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNRYE
CCEEEECCCCCCCCCHHHHHHEEECCCHHHHCCCCCCCCHHHHHHCCCCCCCEEEEHHHH
NFEKFMTAKTTKRIISFSKKNGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIF
HHHHHHHHHHHHHHHHHHCCCCEEEEECEECCCCEEEEECCCCCCCHHHCCCCCEEEEEE
MPNIQTGNNDQKGVRSLNLSVACGIAIYEAHKQINFQNGN
ECCCCCCCCCCCHHEEECEEEEECEEEEEHHHEEEECCCC
>Mature Secondary Structure
MEVALFEPRIPQNTGNIARSCAAFDIPLNLIEPLGFKLEDKYLKRAGLDYWPLVTVNRYE
CCEEEECCCCCCCCCHHHHHHEEECCCHHHHCCCCCCCCHHHHHHCCCCCCCEEEEHHHH
NFEKFMTAKTTKRIISFSKKNGLYLKDFKFKQDDILLFGREDSGLPDSIIDKSDFLISIF
HHHHHHHHHHHHHHHHHHCCCCEEEEECEECCCCEEEEECCCCCCCHHHCCCCCEEEEEE
MPNIQTGNNDQKGVRSLNLSVACGIAIYEAHKQINFQNGN
ECCCCCCCCCCCHHEEECEEEEECEEEEEHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA