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The map label for this gene is dapF [H]

Identifier: 126696309

GI number: 126696309

Start: 840497

End: 841357

Strand: Direct

Name: dapF [H]

Synonym: P9301_09711

Alternate gene names: 126696309

Gene position: 840497-841357 (Clockwise)

Preceding gene: 126696307

Following gene: 126696310

Centisome position: 51.19

GC content: 29.15

Gene sequence:

>861_bases
ATGAAAAATATAACTTTTGAAAAATATCAAGGTAACGGAAATGATTTCGTAGTAATTGATTCTAGAGGAAATGATTTATA
TAAAAATTACAAGACAAATAAAATATTTGATATAAAACAAATTTGCAACAGGCAATTTGGTATTGGAGCAGATGGTGTGA
TTTTTATAGAAGAACCTAAAGAAGATAATTATGCAAAAATGATAATCTTTAATTCTGATGGTTCTGAAGCACAAATGTGT
GGAAACGGAATTAGGTGTTTAGTTGAGTATCTCCACCTAAATGATTCAATGAATAATAAAAATATAGAATATAAAATTGA
GACTAAAGCAGGTTTAAAAATCGCAAAATATATAAATGATGAAATTACAGTAAAAATGGGAGTTCCAATTTTAGAATGTC
AAAATATTCCAACAACAATTGAAAAAAAAATAAATTCAATTCCTTCACACGAATTTATTGAGAAAGATTTTAATAATATG
GGTTATGCCGTAGGAATGGGCAATCCTCATTTAATATTCTTTGTAAAAGACATAGAGTCAATTATTCTTTCCAGACTTGG
TCCTATACTAGAAAAAAATGAATTATTTCCTGAAAAAACTAATGTGCATTTTTGTCAAATTTTAAATAGAGATAATATCA
AAGTAAAAGTATGGGAAAGGGGTGCAGGACCAACCTTAGCTTGTGGAACAGGTGCCTGTGCTATTCATGTAGCAGCTTAT
AAATTAGGCCTTTGTAATTCACAAACCATAGTAACTTTACCAGGAGGTAATCTTAAAATTGATTGGTCAAAAGACGATTG
TGAAGTCATGATGACCGGTAATGCTAAAAAGGTTTTCTCAGGATCAATGCTAGTAAATTAA

Upstream 100 bases:

>100_bases
TTATTTGTTAAATTATTATTTTAATATCCATTGTATAAAATAGAAATAGATTGTTAAAAGGAATAATTTATAATTAAAAT
TTAAAATATATTATCTACCA

Downstream 100 bases:

>100_bases
TGGAAAATAATTTTATCTATTTAGATAATGCATCCACAACTCCATTATCTGAGAATGTTTTAAATATAATAAATTCAACT
TATAGGGATTATTGGCATAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKNITFEKYQGNGNDFVVIDSRGNDLYKNYKTNKIFDIKQICNRQFGIGADGVIFIEEPKEDNYAKMIIFNSDGSEAQMC
GNGIRCLVEYLHLNDSMNNKNIEYKIETKAGLKIAKYINDEITVKMGVPILECQNIPTTIEKKINSIPSHEFIEKDFNNM
GYAVGMGNPHLIFFVKDIESIILSRLGPILEKNELFPEKTNVHFCQILNRDNIKVKVWERGAGPTLACGTGACAIHVAAY
KLGLCNSQTIVTLPGGNLKIDWSKDDCEVMMTGNAKKVFSGSMLVN

Sequences:

>Translated_286_residues
MKNITFEKYQGNGNDFVVIDSRGNDLYKNYKTNKIFDIKQICNRQFGIGADGVIFIEEPKEDNYAKMIIFNSDGSEAQMC
GNGIRCLVEYLHLNDSMNNKNIEYKIETKAGLKIAKYINDEITVKMGVPILECQNIPTTIEKKINSIPSHEFIEKDFNNM
GYAVGMGNPHLIFFVKDIESIILSRLGPILEKNELFPEKTNVHFCQILNRDNIKVKVWERGAGPTLACGTGACAIHVAAY
KLGLCNSQTIVTLPGGNLKIDWSKDDCEVMMTGNAKKVFSGSMLVN
>Mature_286_residues
MKNITFEKYQGNGNDFVVIDSRGNDLYKNYKTNKIFDIKQICNRQFGIGADGVIFIEEPKEDNYAKMIIFNSDGSEAQMC
GNGIRCLVEYLHLNDSMNNKNIEYKIETKAGLKIAKYINDEITVKMGVPILECQNIPTTIEKKINSIPSHEFIEKDFNNM
GYAVGMGNPHLIFFVKDIESIILSRLGPILEKNELFPEKTNVHFCQILNRDNIKVKVWERGAGPTLACGTGACAIHVAAY
KLGLCNSQTIVTLPGGNLKIDWSKDDCEVMMTGNAKKVFSGSMLVN

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=33.6879432624113, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32037; Mature: 32037

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNITFEKYQGNGNDFVVIDSRGNDLYKNYKTNKIFDIKQICNRQFGIGADGVIFIEEPK
CCCCEEEEEECCCCEEEEEECCCCHHHHCCCCCEEEEHHHHHCCCCCCCCCCEEEEECCC
EDNYAKMIIFNSDGSEAQMCGNGIRCLVEYLHLNDSMNNKNIEYKIETKAGLKIAKYIND
CCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCC
EITVKMGVPILECQNIPTTIEKKINSIPSHEFIEKDFNNMGYAVGMGNPHLIFFVKDIES
EEEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEEEHHHHH
IILSRLGPILEKNELFPEKTNVHFCQILNRDNIKVKVWERGAGPTLACGTGACAIHVAAY
HHHHHHCCHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCEEEEEEEE
KLGLCNSQTIVTLPGGNLKIDWSKDDCEVMMTGNAKKVFSGSMLVN
EEECCCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEECCCEECC
>Mature Secondary Structure
MKNITFEKYQGNGNDFVVIDSRGNDLYKNYKTNKIFDIKQICNRQFGIGADGVIFIEEPK
CCCCEEEEEECCCCEEEEEECCCCHHHHCCCCCEEEEHHHHHCCCCCCCCCCEEEEECCC
EDNYAKMIIFNSDGSEAQMCGNGIRCLVEYLHLNDSMNNKNIEYKIETKAGLKIAKYIND
CCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCC
EITVKMGVPILECQNIPTTIEKKINSIPSHEFIEKDFNNMGYAVGMGNPHLIFFVKDIES
EEEEEECCCEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEEEHHHHH
IILSRLGPILEKNELFPEKTNVHFCQILNRDNIKVKVWERGAGPTLACGTGACAIHVAAY
HHHHHHCCHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCEEEEEEEE
KLGLCNSQTIVTLPGGNLKIDWSKDDCEVMMTGNAKKVFSGSMLVN
EEECCCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA