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The map label for this gene is ispE

Identifier: 126696264

GI number: 126696264

Start: 796714

End: 797649

Strand: Direct

Name: ispE

Synonym: P9301_09261

Alternate gene names: 126696264

Gene position: 796714-797649 (Clockwise)

Preceding gene: 126696263

Following gene: 126696265

Centisome position: 48.52

GC content: 27.56

Gene sequence:

>936_bases
ATGCAAGATTTAGCTAAACCGAAAATTAAGATAAAATCTCCTGCCAAAATAAATTTGCACCTTGAAGTTATTGGTAAAAG
AGAGGATGGATTTCATGAGTTAGCAATGATTATGCAAAATATCGATCTTTCTGATTATTTAGAATTTGAAATAAATAATG
AAGGTTTAATTAAACTTGAGTCTGATTGTAATGATTTAAGCTTGTCTGATGATAACTTAATTGTTAAATCGGCAAATCTA
TTAAGAAAAAATTCAAATATAAATTACGGTGCGAATATATTTTTAAGAAAAAATATTCCAATTGGCGCAGGATTAGCTGG
TGGATCCAGTAATGCAGCAGCAACATTAATTGGTCTTAATAAGTTATGGAATTTGGACTTAGATCATGGAACATTATGTT
CATTAGCATCAACTTTAGGATCTGATATTCCCTTTTTTATAAATGGTGGCATTCAGTTATGTTTTGGAAGAGGAGAAATT
TTGGAGAAATTAGATTCAAACTTTGAATATGGAGTAATTCTTTTAAAAAATCCAAATGTATCAGTATCTACAGCTGAAAC
TTATAAAAAATATAGTAATAGATTTTGTGATAATCATCTTAATGATAGAAAAATGATTGAGAACATAAGAAAAAATTTAA
GGGATAATGGTTTGAATAAATTAAATTTTGATAATCAACATTTATTTATTAAAAATGATTTGCAGTTAGTTGTTGAAAAT
GAAAATGATTCTGTAAAGCAGGCATTATATTTACTTTCTAAACTAGAAAATTGTCTCACATTTTCAATGAGTGGATCAGG
ACCTACATGCTTTGCACTCTTTAAAGATATAGAGACTGCAAAAAAAGAATTAACTGCTAATTCTAAATTTTTTAAAGATA
AAGGCTATGATTCATGGGTTTGCACTTTCCTTGAAAAGGGAATAACATTCATTTAA

Upstream 100 bases:

>100_bases
AAAGTTTGTTTTAATTTAAGACCACAAGATATTTCAATTGATCAATGGATTAAGCTTGCAGAAAATTGTATTAAAATTAA
AAAATAAAAATTTTAAGTAT

Downstream 100 bases:

>100_bases
ATTTTTATATAAAATCTATTTTGGCTGATAATAGTAATGAGAATATTGAAAAAAATATTCCCGAGAAAGGACCTTTAAAT
TTTATTGTTGGATCATTAAC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MQDLAKPKIKIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLESDCNDLSLSDDNLIVKSANL
LRKNSNINYGANIFLRKNIPIGAGLAGGSSNAAATLIGLNKLWNLDLDHGTLCSLASTLGSDIPFFINGGIQLCFGRGEI
LEKLDSNFEYGVILLKNPNVSVSTAETYKKYSNRFCDNHLNDRKMIENIRKNLRDNGLNKLNFDNQHLFIKNDLQLVVEN
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANSKFFKDKGYDSWVCTFLEKGITFI

Sequences:

>Translated_311_residues
MQDLAKPKIKIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLESDCNDLSLSDDNLIVKSANL
LRKNSNINYGANIFLRKNIPIGAGLAGGSSNAAATLIGLNKLWNLDLDHGTLCSLASTLGSDIPFFINGGIQLCFGRGEI
LEKLDSNFEYGVILLKNPNVSVSTAETYKKYSNRFCDNHLNDRKMIENIRKNLRDNGLNKLNFDNQHLFIKNDLQLVVEN
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANSKFFKDKGYDSWVCTFLEKGITFI
>Mature_311_residues
MQDLAKPKIKIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLESDCNDLSLSDDNLIVKSANL
LRKNSNINYGANIFLRKNIPIGAGLAGGSSNAAATLIGLNKLWNLDLDHGTLCSLASTLGSDIPFFINGGIQLCFGRGEI
LEKLDSNFEYGVILLKNPNVSVSTAETYKKYSNRFCDNHLNDRKMIENIRKNLRDNGLNKLNFDNQHLFIKNDLQLVVEN
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANSKFFKDKGYDSWVCTFLEKGITFI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=307, Percent_Identity=31.2703583061889, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PROM0 (A3PCS4)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091150.1
- ProteinModelPortal:   A3PCS4
- SMR:   A3PCS4
- STRING:   A3PCS4
- GeneID:   4912314
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_09261
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   PRK02534
- BioCyc:   PMAR167546:P9301ORF_0943-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34832; Mature: 34832

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 16-16 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLAKPKIKIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLE
CCCCCCCCEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEC
SDCNDLSLSDDNLIVKSANLLRKNSNINYGANIFLRKNIPIGAGLAGGSSNAAATLIGLN
CCCCCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEHH
KLWNLDLDHGTLCSLASTLGSDIPFFINGGIQLCFGRGEILEKLDSNFEYGVILLKNPNV
HHEECCCCCCHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHCCCCCEEEEEEECCCC
SVSTAETYKKYSNRFCDNHLNDRKMIENIRKNLRDNGLNKLNFDNQHLFIKNDLQLVVEN
EEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEEC
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANSKFFKDKGYDSWV
CCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
CTFLEKGITFI
HHHHHCCCCCC
>Mature Secondary Structure
MQDLAKPKIKIKSPAKINLHLEVIGKREDGFHELAMIMQNIDLSDYLEFEINNEGLIKLE
CCCCCCCCEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEEC
SDCNDLSLSDDNLIVKSANLLRKNSNINYGANIFLRKNIPIGAGLAGGSSNAAATLIGLN
CCCCCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEHH
KLWNLDLDHGTLCSLASTLGSDIPFFINGGIQLCFGRGEILEKLDSNFEYGVILLKNPNV
HHEECCCCCCHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHHHCCCCCEEEEEEECCCC
SVSTAETYKKYSNRFCDNHLNDRKMIENIRKNLRDNGLNKLNFDNQHLFIKNDLQLVVEN
EEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEEC
ENDSVKQALYLLSKLENCLTFSMSGSGPTCFALFKDIETAKKELTANSKFFKDKGYDSWV
CCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
CTFLEKGITFI
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA