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The map label for this gene is ksgA [H]

Identifier: 126696263

GI number: 126696263

Start: 795876

End: 796700

Strand: Direct

Name: ksgA [H]

Synonym: P9301_09251

Alternate gene names: 126696263

Gene position: 795876-796700 (Clockwise)

Preceding gene: 126696257

Following gene: 126696264

Centisome position: 48.47

GC content: 24.85

Gene sequence:

>825_bases
ATGAATTCTAAAAACTATCATCAAAAAAAAAGATTTGGACAACATTGGTTGGTCAATAAAAAAATATTAGAAAAAATTAA
AGAAATTGCTGTTCTTAATGAAAATGACTTTATTTTAGAAATTGGTCCTGGTAAAGGAGCTTTAACATCTAAGTTATTAA
ATTCAGAAATTAAAAAATTACATGCAATTGAATTAGATAAAGATTTAATAAATTTATTAAATGATAAATTCAATAATAAT
GATAAGTTTTCACTGCAGCAGGGAGATATTCTCTCTGTTAATTTAGATTCGATCAATAAGAAGATTACAAAAGTAATTGC
AAATATTCCTTACAATATAACTGGTCCAATATTGGATATTTTTATAGGTAGATTGGGCATTATAAGAAAATATAATTACG
AAAAAATAATATTTTTAATGCAGAAAGATGTTGTAGATAGGATTTTGTCAAAAGAAGGTAGTCCTAATGCTGGTGCGCTT
AGTATAAGAATGCAACTTTTATCAAAAATAAAAAGAATATGTGATGTGCCCCCTTCATCATTTAGTCCACCGCCAAAAGT
TTTTTCTTCTTTAGTAGTTTTCGAACCAATTAAAAATGATTTAAGATTAGATATTAGTCTAGAAAAATATATAGATAAAC
TTCTTCGAATTTCATTTAATTCAAGAAGGAAAATGCTTAGAAATACTCTTAATACAATACTTTCGAATGAAGAGATAAAT
GAATTATCTGAATCTTCAAAAGTTTGTTTTAATTTAAGACCACAAGATATTTCAATTGATCAATGGATTAAGCTTGCAGA
AAATTGTATTAAAATTAAAAAATAA

Upstream 100 bases:

>100_bases
TTAAATTTATTGAGTTTATCTAAATTTTTTTTTGATAAGCTCATGATATAAAGTATTAAATAAATTCATATTTAATTTAA
ACATATTATTTATCTTTTAG

Downstream 100 bases:

>100_bases
AAATTTTAAGTATATGCAAGATTTAGCTAAACCGAAAATTAAGATAAAATCTCCTGCCAAAATAAATTTGCACCTTGAAG
TTATTGGTAAAAGAGAGGAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLNSEIKKLHAIELDKDLINLLNDKFNNN
DKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDIFIGRLGIIRKYNYEKIIFLMQKDVVDRILSKEGSPNAGAL
SIRMQLLSKIKRICDVPPSSFSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNTILSNEEIN
ELSESSKVCFNLRPQDISIDQWIKLAENCIKIKK

Sequences:

>Translated_274_residues
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLNSEIKKLHAIELDKDLINLLNDKFNNN
DKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDIFIGRLGIIRKYNYEKIIFLMQKDVVDRILSKEGSPNAGAL
SIRMQLLSKIKRICDVPPSSFSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNTILSNEEIN
ELSESSKVCFNLRPQDISIDQWIKLAENCIKIKK
>Mature_274_residues
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLNSEIKKLHAIELDKDLINLLNDKFNNN
DKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDIFIGRLGIIRKYNYEKIIFLMQKDVVDRILSKEGSPNAGAL
SIRMQLLSKIKRICDVPPSSFSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNTILSNEEIN
ELSESSKVCFNLRPQDISIDQWIKLAENCIKIKK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=235, Percent_Identity=33.1914893617021, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=23.758865248227, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=28.6792452830189, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=27.1477663230241, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25146882, Length=188, Percent_Identity=31.3829787234043, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=29.7169811320755, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358017, Length=224, Percent_Identity=31.6964285714286, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=25.9927797833935, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31487; Mature: 31487

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLNSEIKKL
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHH
HAIELDKDLINLLNDKFNNNDKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHH
FIGRLGIIRKYNYEKIIFLMQKDVVDRILSKEGSPNAGALSIRMQLLSKIKRICDVPPSS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
FSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNTILSNEEIN
CCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHH
ELSESSKVCFNLRPQDISIDQWIKLAENCIKIKK
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSKNYHQKKRFGQHWLVNKKILEKIKEIAVLNENDFILEIGPGKGALTSKLLNSEIKKL
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHH
HAIELDKDLINLLNDKFNNNDKFSLQQGDILSVNLDSINKKITKVIANIPYNITGPILDI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHH
FIGRLGIIRKYNYEKIIFLMQKDVVDRILSKEGSPNAGALSIRMQLLSKIKRICDVPPSS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
FSPPPKVFSSLVVFEPIKNDLRLDISLEKYIDKLLRISFNSRRKMLRNTLNTILSNEEIN
CCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHH
ELSESSKVCFNLRPQDISIDQWIKLAENCIKIKK
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA