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The map label for this gene is ilvB [H]

Identifier: 126695890

GI number: 126695890

Start: 482029

End: 483792

Strand: Direct

Name: ilvB [H]

Synonym: P9301_05521

Alternate gene names: 126695890

Gene position: 482029-483792 (Clockwise)

Preceding gene: 126695889

Following gene: 126695891

Centisome position: 29.36

GC content: 36.73

Gene sequence:

>1764_bases
GTGACCCTTACTTCGAGATCCTTTTCACAGGGTAGTTCAAAACATGAAAATCCAGTTTGGATAACTGGTGCAGATGCACT
AATGGATTCTTTAAAAATTCATGGAGTAAAAGTTATATTTGGATATCCTGGCGGAGCCATATTACCAATATACGACGCTG
TTCATAAGGCAGAACAAGATGGTTGGTTAAAACACTATATGGTTCGGCATGAACAAGGTGGTTCACATGCGGCTGATGGA
TATGCAAGATCTACTGGTGAAGTAGGAGTATGTTTTGGAACCTCAGGCCCAGGTGCAACAAATTTGGTAACTGGAATTGC
TACTGCGCAAATGGATTCAGTCCCTCTAGTTGTAGTTACAGGTCAAGTCCCAAGACCTGCTATAGGAACAGATGCTTTTC
AAGAAACTGATATTTTTGGTATAACTCTTCCAATAGTGAAACATTCATGGGTAATAAGGGATCCTTCAGATATCGCGAAA
GTAGTTTCTGAAGCTTTTTTTATAGCCTCATCTGGAAGGCCTGGCCCTGTTTTAATTGATATACCCAAAGATGTAGGTCA
GGAGTTCTTTAATTACCAAAGAGTTTTGCCAGGTGAGATTATTCCAAAAGGATTTAAAAGGAATGGAGAAATTAATGATT
GCGATATCAACAAAGCAATTAAATTAATAGAAGATTCTAAAAGACCTCTTCTATACGTAGGAGGTGGGGCTATATCTTCA
GGAGCTCATGATGAAATAAAAACTTTGGCAAAGAATTATCAAATACCAGTTACCACAACCTTAATGGGAAAGGGTGCTTT
CGATGAAAAAGATAATTTATCAGTAGGAATGTTAGGAATGCATGGAACTGCTTATGCAAATTTTGCTGTTACAGAATGCG
ATCTTTTAATTGCTATTGGAGCTAGATTCGATGATAGGGTGACAGGTAAATTAGATACTTTTGCACCTAATGCAAAGGTG
ATTCATATAGATATTGACCCGGCAGAAGTTAATAAAAATAGGCGTGTAGATGTTGCAATTGTTGCTGATGTTTCAAAAGC
TGTTCTGAAAATTAATGAACAATCTCTAAAAAACAAATTTACTTGCCAGACGAAAAACTGGTTAGAAAAAATTGATTTTT
GGAAACATAAACACCCTTTATATGACCCGCCTAAAGAAGGAGAAATTTATCCTCAGGAAGTTCTTTTAAAAGTGAGGGAA
CTTTTACCAGAAGCTTATATAACTACAGATGTAGGACAACATCAAATGTGGGCTGCTCAATATCTTAGGAATTCTCCAAG
AAAATGGATTAGTAGTGCAGGCTTAGGAACTATGGGTTTTGGATTGCCAGCGGCAATTGGAGTAAAAGCAGCCTTACCTA
ATTCAGATGTAATTTGTATTGCAGGAGATGCAAGTGTCTTAATGAATATTCAAGAATTGGGAACCTTATCTCAATACGGT
CTAAAGGTGAAATTGATTATTATTAATAATCGCTGGCAAGGGATGGTAAGACAATGGCAAGAAAGTTTCTATGATGAAAG
ATATTCCTCATCTGATATGAGTTGTGGAGAACCTGATTTTGTAAAACTTGCTGAGTCTTTTGGGGTTAAAGGATACCTAA
TTTCTGATAGAAAAGAATTACAAAATGACTTAAAAAATGCGTTTGATCATGACGGACCTGCCTTGATTAATATCCTTGTC
AGAAGAGGTGAAAATTGTTATCCAATGGTTCCTCCTGGGAAAAGTAATGCTCAAATGGTTGGATATGTTAATTGTGAAGA
CTAA

Upstream 100 bases:

>100_bases
TAAAAGATTTTTTTTGAATATATTTCTGACATTACATTTCTAAATGAGGCAAAAGTATTTAATTGAGTTTAATATTTATA
TTAAATATTGAATTTCTTTA

Downstream 100 bases:

>100_bases
TTTTTTTAAAATTCATCATTTTAAAAAATTAACTTTAAGATAATTTAAAAACTTAGATATTTCTGAATTGAAAAAATTAT
CAAAAATTTTAATTATAATC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTLTSRSFSQGSSKHENPVWITGADALMDSLKIHGVKVIFGYPGGAILPIYDAVHKAEQDGWLKHYMVRHEQGGSHAADG
YARSTGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAK
VVSEAFFIASSGRPGPVLIDIPKDVGQEFFNYQRVLPGEIIPKGFKRNGEINDCDINKAIKLIEDSKRPLLYVGGGAISS
GAHDEIKTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPNAKV
IHIDIDPAEVNKNRRVDVAIVADVSKAVLKINEQSLKNKFTCQTKNWLEKIDFWKHKHPLYDPPKEGEIYPQEVLLKVRE
LLPEAYITTDVGQHQMWAAQYLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
LKVKLIIINNRWQGMVRQWQESFYDERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKELQNDLKNAFDHDGPALINILV
RRGENCYPMVPPGKSNAQMVGYVNCED

Sequences:

>Translated_587_residues
MTLTSRSFSQGSSKHENPVWITGADALMDSLKIHGVKVIFGYPGGAILPIYDAVHKAEQDGWLKHYMVRHEQGGSHAADG
YARSTGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAK
VVSEAFFIASSGRPGPVLIDIPKDVGQEFFNYQRVLPGEIIPKGFKRNGEINDCDINKAIKLIEDSKRPLLYVGGGAISS
GAHDEIKTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPNAKV
IHIDIDPAEVNKNRRVDVAIVADVSKAVLKINEQSLKNKFTCQTKNWLEKIDFWKHKHPLYDPPKEGEIYPQEVLLKVRE
LLPEAYITTDVGQHQMWAAQYLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
LKVKLIIINNRWQGMVRQWQESFYDERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKELQNDLKNAFDHDGPALINILV
RRGENCYPMVPPGKSNAQMVGYVNCED
>Mature_586_residues
TLTSRSFSQGSSKHENPVWITGADALMDSLKIHGVKVIFGYPGGAILPIYDAVHKAEQDGWLKHYMVRHEQGGSHAADGY
ARSTGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVTGQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAKV
VSEAFFIASSGRPGPVLIDIPKDVGQEFFNYQRVLPGEIIPKGFKRNGEINDCDINKAIKLIEDSKRPLLYVGGGAISSG
AHDEIKTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPNAKVI
HIDIDPAEVNKNRRVDVAIVADVSKAVLKINEQSLKNKFTCQTKNWLEKIDFWKHKHPLYDPPKEGEIYPQEVLLKVREL
LPEAYITTDVGQHQMWAAQYLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYGL
KVKLIIINNRWQGMVRQWQESFYDERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKELQNDLKNAFDHDGPALINILVR
RGENCYPMVPPGKSNAQMVGYVNCED

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=27.5, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI21361361, Length=591, Percent_Identity=24.8730964467005, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=45.6989247311828, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=43.3392539964476, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=572, Percent_Identity=31.2937062937063, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=27.4021352313167, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=24.8638838475499, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=591, Percent_Identity=27.2419627749577, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=24.9566724436742, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=582, Percent_Identity=25.085910652921, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=42.0783645655877, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=24.0890688259109, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=513, Percent_Identity=22.4171539961014, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=26.3716814159292, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64313; Mature: 64182

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTSRSFSQGSSKHENPVWITGADALMDSLKIHGVKVIFGYPGGAILPIYDAVHKAEQD
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHEECEEEEEEECCCCCEEHHHHHHHHHCCC
GWLKHYMVRHEQGGSHAADGYARSTGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVT
CHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAKVVSEAFFIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEEHHCCEEEEECHHHHHHHHHHHEEEECCCCCCCEEEE
IPKDVGQEFFNYQRVLPGEIIPKGFKRNGEINDCDINKAIKLIEDSKRPLLYVGGGAISS
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCC
GAHDEIKTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIG
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECEEEEEEC
ARFDDRVTGKLDTFAPNAKVIHIDIDPAEVNKNRRVDVAIVADVSKAVLKINEQSLKNKF
CCCCCCCCCCEECCCCCCEEEEEECCHHHCCCCCEEEEEEEEHHHHHHHHHCHHHHCCCE
TCQTKNWLEKIDFWKHKHPLYDPPKEGEIYPQEVLLKVRELLPEAYITTDVGQHQMWAAQ
EECHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEECCCCCHHHHHHH
YLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
HHHCCHHHHHHHCCCCCCCCCCHHHHCEEEECCCCCEEEEECCHHHHHHHHHHCCHHHCC
LKVKLIIINNRWQGMVRQWQESFYDERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKEL
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHH
QNDLKNAFDHDGPALINILVRRGENCYPMVPPGKSNAQMVGYVNCED
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TLTSRSFSQGSSKHENPVWITGADALMDSLKIHGVKVIFGYPGGAILPIYDAVHKAEQD
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHEECEEEEEEECCCCCEEHHHHHHHHHCCC
GWLKHYMVRHEQGGSHAADGYARSTGEVGVCFGTSGPGATNLVTGIATAQMDSVPLVVVT
CHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVIRDPSDIAKVVSEAFFIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEEHHCCEEEEECHHHHHHHHHHHEEEECCCCCCCEEEE
IPKDVGQEFFNYQRVLPGEIIPKGFKRNGEINDCDINKAIKLIEDSKRPLLYVGGGAISS
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCC
GAHDEIKTLAKNYQIPVTTTLMGKGAFDEKDNLSVGMLGMHGTAYANFAVTECDLLIAIG
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECEEEEEEC
ARFDDRVTGKLDTFAPNAKVIHIDIDPAEVNKNRRVDVAIVADVSKAVLKINEQSLKNKF
CCCCCCCCCCEECCCCCCEEEEEECCHHHCCCCCEEEEEEEEHHHHHHHHHCHHHHCCCE
TCQTKNWLEKIDFWKHKHPLYDPPKEGEIYPQEVLLKVRELLPEAYITTDVGQHQMWAAQ
EECHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEECCCCCHHHHHHH
YLRNSPRKWISSAGLGTMGFGLPAAIGVKAALPNSDVICIAGDASVLMNIQELGTLSQYG
HHHCCHHHHHHHCCCCCCCCCCHHHHCEEEECCCCCEEEEECCHHHHHHHHHHCCHHHCC
LKVKLIIINNRWQGMVRQWQESFYDERYSSSDMSCGEPDFVKLAESFGVKGYLISDRKEL
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHH
QNDLKNAFDHDGPALINILVRRGENCYPMVPPGKSNAQMVGYVNCED
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]