Click here to switch to the map view.

The map label for this gene is mrcB [H]

Identifier: 126695789

GI number: 126695789

Start: 391819

End: 393588

Strand: Reverse

Name: mrcB [H]

Synonym: P9301_04511

Alternate gene names: 126695789

Gene position: 393588-391819 (Counterclockwise)

Preceding gene: 126695790

Following gene: 126695780

Centisome position: 23.97

GC content: 27.34

Gene sequence:

>1770_bases
GTGCAAAAGATTAAATTCAAATCTTTTGTTTTAATAATTCCAATATTAATTTTTTCTGGAATATCTTTTTATTTTTTTAG
TTTAATATTTAGTACCTTAAAATTTGACGTTTCTAAAAATAAAAAGTCTCGGGAAACCAAATATTCTTACGTAATATCAT
CTTCTGATAATAAGATACTAAGCAAATTAAGCCGCAAATTTGAAATAGATAATAATCATCATAAAATTCCATTTTTTATT
AAACATTCTTTTATATCTTCTGAGGATAAAAGATTTTATAAACATAATGGAATAGATTTAAAAAGTATTTCACGTGCCCT
TTTCCAAAATATTAGAAGTGGTTATGTCAAAGAGGGAGGAAGTACGATTACACAACAAGTTGCTAGATTACTTTTTCTAA
ATAATGATCTAAGTTTTCAAAGAAAAATCAAAGAAATATTTATATCACTCATACTTGAATTTAGATATAACAAAAATCAA
ATTTTAAAATTATATTTAAATAATATTTATCTAGGATCAGGAGCATACGGGGTAGACGAGGCTGCCCAAGTTTATTTTGG
AAAATTTATAGAAGAATTGACATTATCAGAGATCGCATTAATTGCAGGATTAGCTCCAGCTCCATCAATTTATTCACCTT
ATCAAAATTTAGAACTAGCTATTGAAAATAGAAATAAAGTTCTTGAATCAATGTATGTTGATGGATATATTTCTCATACA
AATAAGAATAAGGCTATTAAAGAAAAAATAAAGTTAAATTATCAAACAGCTGATAATTTTTTAGATGATAAATTACTAAT
AAACTTTATTCTTCAGGAAACAGATAAAAAAATTGGAAGTAAAAATGATTATAAGTTTTTAAGAATTAAATCTTCTATCA
ACAAAGATTGGCAAGAAAAAGGCCAAAAAATATCAAGGTACGCAGGTCCTAAAGAACTTGAATTTGGTCTGTTATCTATC
GAATCAAACACAGGACTAATCAGGACAATGATAACCAGCAAAAATCCAGCAATTAATGAATACAATAGAGTGATTACATC
TGTAAGACCTTTAGCTTCTACTTTTAAAATAATTCCTTATGCTGCTGCATTAATAGAAGGAATAAAATTAAGCGATAAAT
TTGAAGACTTACCAACATGCTGGGAAAGTTATTGTCCAAAAAATTTTTCAGAAGACTATAGAGGTTCAATATCTTTGATT
GAATCATTTAAAAGTTCCTCAAATATCGTTCCAATATCAATAACAAAAAAAATCGGCTTAAAAAATATTATTAATTTAGC
GAATTCATTTGGACTAGGATACGAGCAAGAGTTTGAGGAATTCCCAGCATTAGCTATTGGCGCCTACGGAGATAATCTTT
TAAACATCACAAATGCATATTCTGCAATAAACAATAATGGGAAAATTCAAAGCCCTGAAATCATAGAAAAAATAGAATCA
TTTAAAAAACAACCTATTTGGGAAAATAAATCTATTTCCAAGAAAATATTAGATTTAAAAATAAACAAGAAATTAAATAA
ACTTCTACAAAATTCTGTAAAGGAAGGCACTTCAAAAGCAGCCTCTATTAAAGGAAAGAAAATTTATGGGAAAACAGGAA
CATCTGATGGAAATAAAGATCTCTGGTTTATTGGTTCCATTGATAACCTTACAACAGGGATCTGGATAGGTTACGACGAT
AACAAGGAATCTGAATTATCTAGTGGGAATGCAGCTTATTTATGGAAGAAGTTCATATCTGAAATTTATAAAATTCCAAT
TAAAAAATAA

Upstream 100 bases:

>100_bases
AAGTTTGATGTCAAATATCAAGCAAGTGCCCAACCATTCCTTATAACTGGAATGTTAGTTACAGCCTTAGCAATAGGACA
TAGTTTTTTAGTTGCTTAAG

Downstream 100 bases:

>100_bases
ATTATATTTTTAAGATTTATAAGAAATTATTGCAGAATAAAATCCATCGCCAGGGTAATCCAAGCTAGGTAAAATTTGCT
TTTCGCTAACCAATTTTAAA

Product: putative penicillin binding protein

Products: NA

Alternate protein names: PBP 4 [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLIFSTLKFDVSKNKKSRETKYSYVISSSDNKILSKLSRKFEIDNNHHKIPFFI
KHSFISSEDKRFYKHNGIDLKSISRALFQNIRSGYVKEGGSTITQQVARLLFLNNDLSFQRKIKEIFISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVDEAAQVYFGKFIEELTLSEIALIAGLAPAPSIYSPYQNLELAIENRNKVLESMYVDGYISHT
NKNKAIKEKIKLNYQTADNFLDDKLLINFILQETDKKIGSKNDYKFLRIKSSINKDWQEKGQKISRYAGPKELEFGLLSI
ESNTGLIRTMITSKNPAINEYNRVITSVRPLASTFKIIPYAAALIEGIKLSDKFEDLPTCWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKKIGLKNIINLANSFGLGYEQEFEEFPALAIGAYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
FKKQPIWENKSISKKILDLKINKKLNKLLQNSVKEGTSKAASIKGKKIYGKTGTSDGNKDLWFIGSIDNLTTGIWIGYDD
NKESELSSGNAAYLWKKFISEIYKIPIKK

Sequences:

>Translated_589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLIFSTLKFDVSKNKKSRETKYSYVISSSDNKILSKLSRKFEIDNNHHKIPFFI
KHSFISSEDKRFYKHNGIDLKSISRALFQNIRSGYVKEGGSTITQQVARLLFLNNDLSFQRKIKEIFISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVDEAAQVYFGKFIEELTLSEIALIAGLAPAPSIYSPYQNLELAIENRNKVLESMYVDGYISHT
NKNKAIKEKIKLNYQTADNFLDDKLLINFILQETDKKIGSKNDYKFLRIKSSINKDWQEKGQKISRYAGPKELEFGLLSI
ESNTGLIRTMITSKNPAINEYNRVITSVRPLASTFKIIPYAAALIEGIKLSDKFEDLPTCWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKKIGLKNIINLANSFGLGYEQEFEEFPALAIGAYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
FKKQPIWENKSISKKILDLKINKKLNKLLQNSVKEGTSKAASIKGKKIYGKTGTSDGNKDLWFIGSIDNLTTGIWIGYDD
NKESELSSGNAAYLWKKFISEIYKIPIKK
>Mature_589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLIFSTLKFDVSKNKKSRETKYSYVISSSDNKILSKLSRKFEIDNNHHKIPFFI
KHSFISSEDKRFYKHNGIDLKSISRALFQNIRSGYVKEGGSTITQQVARLLFLNNDLSFQRKIKEIFISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVDEAAQVYFGKFIEELTLSEIALIAGLAPAPSIYSPYQNLELAIENRNKVLESMYVDGYISHT
NKNKAIKEKIKLNYQTADNFLDDKLLINFILQETDKKIGSKNDYKFLRIKSSINKDWQEKGQKISRYAGPKELEFGLLSI
ESNTGLIRTMITSKNPAINEYNRVITSVRPLASTFKIIPYAAALIEGIKLSDKFEDLPTCWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKKIGLKNIINLANSFGLGYEQEFEEFPALAIGAYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
FKKQPIWENKSISKKILDLKINKKLNKLLQNSVKEGTSKAASIKGKKIYGKTGTSDGNKDLWFIGSIDNLTTGIWIGYDD
NKESELSSGNAAYLWKKFISEIYKIPIKK

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=537, Percent_Identity=25.3258845437616, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI87082258, Length=306, Percent_Identity=35.9477124183007, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788867, Length=488, Percent_Identity=28.0737704918033, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1789601, Length=126, Percent_Identity=42.8571428571429, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 67110; Mature: 67110

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIKFKSFVLIIPILIFSGISFYFFSLIFSTLKFDVSKNKKSRETKYSYVISSSDNKIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHH
SKLSRKFEIDNNHHKIPFFIKHSFISSEDKRFYKHNGIDLKSISRALFQNIRSGYVKEGG
HHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
STITQQVARLLFLNNDLSFQRKIKEIFISLILEFRYNKNQILKLYLNNIYLGSGAYGVDE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHEEECCCCCCCHH
AAQVYFGKFIEELTLSEIALIAGLAPAPSIYSPYQNLELAIENRNKVLESMYVDGYISHT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCEEECC
NKNKAIKEKIKLNYQTADNFLDDKLLINFILQETDKKIGSKNDYKFLRIKSSINKDWQEK
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHH
GQKISRYAGPKELEFGLLSIESNTGLIRTMITSKNPAINEYNRVITSVRPLASTFKIIPY
HHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAALIEGIKLSDKFEDLPTCWESYCPKNFSEDYRGSISLIESFKSSSNIVPISITKKIGL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEHHHHHH
KNIINLANSFGLGYEQEFEEFPALAIGAYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
HHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FKKQPIWENKSISKKILDLKINKKLNKLLQNSVKEGTSKAASIKGKKIYGKTGTSDGNKD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEECCCCCCCCCCC
LWFIGSIDNLTTGIWIGYDDNKESELSSGNAAYLWKKFISEIYKIPIKK
EEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQKIKFKSFVLIIPILIFSGISFYFFSLIFSTLKFDVSKNKKSRETKYSYVISSSDNKIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHH
SKLSRKFEIDNNHHKIPFFIKHSFISSEDKRFYKHNGIDLKSISRALFQNIRSGYVKEGG
HHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
STITQQVARLLFLNNDLSFQRKIKEIFISLILEFRYNKNQILKLYLNNIYLGSGAYGVDE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHEEECCCCCCCHH
AAQVYFGKFIEELTLSEIALIAGLAPAPSIYSPYQNLELAIENRNKVLESMYVDGYISHT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCEEECC
NKNKAIKEKIKLNYQTADNFLDDKLLINFILQETDKKIGSKNDYKFLRIKSSINKDWQEK
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHH
GQKISRYAGPKELEFGLLSIESNTGLIRTMITSKNPAINEYNRVITSVRPLASTFKIIPY
HHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAALIEGIKLSDKFEDLPTCWESYCPKNFSEDYRGSISLIESFKSSSNIVPISITKKIGL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEHHHHHH
KNIINLANSFGLGYEQEFEEFPALAIGAYGDNLLNITNAYSAINNNGKIQSPEIIEKIES
HHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
FKKQPIWENKSISKKILDLKINKKLNKLLQNSVKEGTSKAASIKGKKIYGKTGTSDGNKD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEECCCCCCCCCCC
LWFIGSIDNLTTGIWIGYDDNKESELSSGNAAYLWKKFISEIYKIPIKK
EEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377 [H]