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The map label for this gene is trmH [H]

Identifier: 126695780

GI number: 126695780

Start: 386592

End: 387263

Strand: Reverse

Name: trmH [H]

Synonym: P9301_04421

Alternate gene names: 126695780

Gene position: 387263-386592 (Counterclockwise)

Preceding gene: 126695789

Following gene: 126695776

Centisome position: 23.59

GC content: 31.1

Gene sequence:

>672_bases
ATGTCAATTTTGCCAAGAAGATTTGAACGAATCAAAAGTGTTTTAGATTGCAGAATGAAAAACTTGACTGTTTTAGTTGA
GGATGTCAATAAACCACATAATTTATCTGCGATATTAAGGACATGTGATGCAGCAGGAGTTTTCGAAGCAAATTTTATTA
GCAAAACGAATGCCGTTAAGACTTTTAATAGTACCGCTCAAGGAAGTCAAAAATGGGTAAAACTGAATAATCATGAAAAC
ACTATCACGGCAATATCTGATTTAAAGAATAAGGGTTTTAAATTATATGGAACAACTCTTAATAGTGAATCAGTAGATTA
TAGAAATTTTGATTATTCTCAAAATACATGTTTTGTTTTAGGAGCAGAAAAATGGGGACTAAGTAATGAACTTAAATCAA
TGGTTGATCAATCAATTTTTATACCAATGAGAGGTATGGTTCAATCTCTGAATGTTTCAGTTGCTGCTTCAATATTATTA
TTTGAAGCTGTTCGCCAAAGAAAAAATAAAGGTATATTGCCAGCTAATGGAGAAGGATTAAACATGGATGAATATCAAAA
AACACTTTTTGAATGGTGTTACCCAGAATTAGCTGCAGTGTATAAAAAATCAGCTAAAGAATATCCAAAGTTGAATGATA
ACGGAGAACTTGATCCTATTACAGATAACTAA

Upstream 100 bases:

>100_bases
CCATTGAGTTAATTTAAACTACCAAAATATTATAAACCAAGAGATTTAATAGGTAATCAATATTTTTTGAATATAATTTT
AAAGTGTGAAAAAATTATAA

Downstream 100 bases:

>100_bases
ATTTATTCAGAATTTTGACTATCGAAAATTTCTTCAAGGTCCTCTCCTATAAAAGAAAGACCTAAAACTAAAAAAAACAT
TGCCAAACCTGGGAACAAAG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHEN
TITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSNELKSMVDQSIFIPMRGMVQSLNVSVAASILL
FEAVRQRKNKGILPANGEGLNMDEYQKTLFEWCYPELAAVYKKSAKEYPKLNDNGELDPITDN

Sequences:

>Translated_223_residues
MSILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHEN
TITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSNELKSMVDQSIFIPMRGMVQSLNVSVAASILL
FEAVRQRKNKGILPANGEGLNMDEYQKTLFEWCYPELAAVYKKSAKEYPKLNDNGELDPITDN
>Mature_222_residues
SILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHENT
ITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSNELKSMVDQSIFIPMRGMVQSLNVSVAASILLF
EAVRQRKNKGILPANGEGLNMDEYQKTLFEWCYPELAAVYKKSAKEYPKLNDNGELDPITDN

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=155, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1790083, Length=218, Percent_Identity=43.1192660550459, Blast_Score=196, Evalue=7e-52,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=36.4864864864865, Blast_Score=81, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355819, Length=151, Percent_Identity=28.476821192053, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25161; Mature: 25030

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVK
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHH
TFNSTAQGSQKWVKLNNHENTITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVL
HCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEE
GAEKWGLSNELKSMVDQSIFIPMRGMVQSLNVSVAASILLFEAVRQRKNKGILPANGEGL
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NMDEYQKTLFEWCYPELAAVYKKSAKEYPKLNDNGELDPITDN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVK
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHH
TFNSTAQGSQKWVKLNNHENTITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVL
HCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEE
GAEKWGLSNELKSMVDQSIFIPMRGMVQSLNVSVAASILLFEAVRQRKNKGILPANGEGL
ECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NMDEYQKTLFEWCYPELAAVYKKSAKEYPKLNDNGELDPITDN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]