Click here to switch to the map view.

The map label for this gene is ndhK

Identifier: 126695655

GI number: 126695655

Start: 292640

End: 293374

Strand: Reverse

Name: ndhK

Synonym: P9301_03171

Alternate gene names: 126695655

Gene position: 293374-292640 (Counterclockwise)

Preceding gene: 126695656

Following gene: 126695654

Centisome position: 17.87

GC content: 36.87

Gene sequence:

>735_bases
TTGAATCCACAATTATCCCCAAAAGCAATAAGAGAAATTCGAGAAGGGACTTGCAACCCTCTTGGTGCACCCCAAGTAAC
TACAGATTTGAGCGAAAATATTATATTGACAAGCTTAGACGATCTTCATAATTGGGCTAGACTAAGCAGTCTTTGGCCTC
TCTTATACGGAACAGCTTGTTGTTTTATAGAATTTGCCGCCTTAATCGGATCTAGATTTGATTTTGATAGATTTGGATTA
GTACCTAGAAGCTCTCCAAGGCAAGCAGATTTGCTAATAGTTGCTGGAACAGTAACTATGAAGATGGCGCCAGCTCTAGT
AAGACTTTATGAGCAAATGCCTGAACCAAAATATGTCATTGCGATGGGTGCATGCACAATCACAGGAGGAATGTTCAGCG
CAGACTCTACAACTGCTGTAAGAGGTGTTGATAAATTGATTCCTGTTGATTTATACCTTCCAGGATGCCCACCAAGACCA
GAAGCAATTTTTGATGCAGTAATCAAATTAAGAAAAAAAGTTGGTAACGAATCAATCTTAGAGCGCACAAAAACTGAACA
AACCCACAGATACATAACATCTGATCATGAAATGAATCTTGTTTTCTCAGAAAATACAGGTGAATATCTAAATAAAACTT
CAGCTAACTCCATTCCATCCTCCAAAAAAGAAAAAATAACTGAACTACCTGATAATAATGAAAAAGCAGAAATTATTGAT
ACTTTAGAAAATTAA

Upstream 100 bases:

>100_bases
GGCTTACTAGCATTTATTGAGGCTTTAATCTTCATTGCAATACTTGTTATTGCCCTGGCATACGCATGGAGAAAAGGTGC
TTTAGAATGGAGTTAAAAAA

Downstream 100 bases:

>100_bases
TGGAAAAAGACGGTTCAGCTAAATCCTCAGATAATTCATTAATAGAAAAAGAAGGCTTTATAAGCCAATCTTTGTCTAAA
GATGGAATTCCAAATCAATC

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANSIPSSKKEKITELPDNNEKAEIID
TLEN

Sequences:

>Translated_244_residues
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANSIPSSKKEKITELPDNNEKAEIID
TLEN
>Mature_244_residues
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTACCFIEFAALIGSRFDFDRFGL
VPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVIAMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRP
EAIFDAVIKLRKKVGNESILERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANSIPSSKKEKITELPDNNEKAEIID
TLEN

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=51.7006802721088, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1788624, Length=164, Percent_Identity=48.1707317073171, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=43.3962264150943, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788834, Length=106, Percent_Identity=42.4528301886792, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=49.6732026143791, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18859983, Length=146, Percent_Identity=53.4246575342466, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24642371, Length=146, Percent_Identity=53.4246575342466, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24651058, Length=143, Percent_Identity=53.1468531468531, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NDHK_PROM0 (A3PB15)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090541.1
- ProteinModelPortal:   A3PB15
- SMR:   A3PB15
- STRING:   A3PB15
- GeneID:   4912687
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_03171
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   ITMKMAP
- ProtClustDB:   PRK06411
- BioCyc:   PMAR167546:P9301ORF_0322-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: 1.6.5.3

Molecular weight: Translated: 27048; Mature: 27048

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
ERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANSIPSSKKEKITELPDNNEKAEIID
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHH
TLEN
HHCC
>Mature Secondary Structure
MNPQLSPKAIREIREGTCNPLGAPQVTTDLSENIILTSLDDLHNWARLSSLWPLLYGTAC
CCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
CFIEFAALIGSRFDFDRFGLVPRSSPRQADLLIVAGTVTMKMAPALVRLYEQMPEPKYVI
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEE
AMGACTITGGMFSADSTTAVRGVDKLIPVDLYLPGCPPRPEAIFDAVIKLRKKVGNESIL
EECCCEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHH
ERTKTEQTHRYITSDHEMNLVFSENTGEYLNKTSANSIPSSKKEKITELPDNNEKAEIID
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHH
TLEN
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA