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The map label for this gene is ndhJ [H]

Identifier: 126695654

GI number: 126695654

Start: 292107

End: 292640

Strand: Reverse

Name: ndhJ [H]

Synonym: P9301_03161

Alternate gene names: 126695654

Gene position: 292640-292107 (Counterclockwise)

Preceding gene: 126695655

Following gene: 126695652

Centisome position: 17.82

GC content: 31.46

Gene sequence:

>534_bases
ATGGAAAAAGACGGTTCAGCTAAATCCTCAGATAATTCATTAATAGAAAAAGAAGGCTTTATAAGCCAATCTTTGTCTAA
AGATGGAATTCCAAATCAATCTTTACCTGATGATCACATAGGAATTGAAAACATTTCTGTGGAACCTAACAAATTATATG
AAGCAGTATCTTTCTTGAGAAATTACGGTTTCAACTATCTCCAATGTCAAGGAGGATATGATGAGGGACCAGGCAAAAAT
CTTGTTAGTTTCTACCATTTTATAACTGTTGATGATTTACAAAAAATTGAAAAAATTAAAGAAGTCAGATTAAAAGTTTT
CTTAAAAAGAGATTCTGATTTATCAATCCCTAGTTTGTATAAAATTTTCAAAGGAAGTGATTGGCAAGAAAGAGAAACTT
TTGATATGTATGGAATAAATTTTATTGATCATCCCAATCCCAAAAGGCTATTAATGCCTGAAGATTGGAGAGGATGGCCA
TTACGAAAAGATTATATCCAGCCGGATTTCTATGAACTTCAAGATGCTTATTAG

Upstream 100 bases:

>100_bases
AAACTTCAGCTAACTCCATTCCATCCTCCAAAAAAGAAAAAATAACTGAACTACCTGATAATAATGAAAAAGCAGAAATT
ATTGATACTTTAGAAAATTA

Downstream 100 bases:

>100_bases
TTTAATTTTTTTAATTTGCGATAAATAAACATAACTGCTCTTGCGAGTCTCCTTGGATCATGTCTAAGAGTTGGAGTTAT
TCTTTTTGAATATAATGGTG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MEKDGSAKSSDNSLIEKEGFISQSLSKDGIPNQSLPDDHIGIENISVEPNKLYEAVSFLRNYGFNYLQCQGGYDEGPGKN
LVSFYHFITVDDLQKIEKIKEVRLKVFLKRDSDLSIPSLYKIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWP
LRKDYIQPDFYELQDAY

Sequences:

>Translated_177_residues
MEKDGSAKSSDNSLIEKEGFISQSLSKDGIPNQSLPDDHIGIENISVEPNKLYEAVSFLRNYGFNYLQCQGGYDEGPGKN
LVSFYHFITVDDLQKIEKIKEVRLKVFLKRDSDLSIPSLYKIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWP
LRKDYIQPDFYELQDAY
>Mature_177_residues
MEKDGSAKSSDNSLIEKEGFISQSLSKDGIPNQSLPDDHIGIENISVEPNKLYEAVSFLRNYGFNYLQCQGGYDEGPGKN
LVSFYHFITVDDLQKIEKIKEVRLKVFLKRDSDLSIPSLYKIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWP
LRKDYIQPDFYELQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI145693162, Length=89, Percent_Identity=42.6966292134831, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010218
- InterPro:   IPR001268
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa [H]

EC number: 1.6.5.3

Molecular weight: Translated: 20658; Mature: 20658

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDGSAKSSDNSLIEKEGFISQSLSKDGIPNQSLPDDHIGIENISVEPNKLYEAVSFLR
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NYGFNYLQCQGGYDEGPGKNLVSFYHFITVDDLQKIEKIKEVRLKVFLKRDSDLSIPSLY
HCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHH
KIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPLRKDYIQPDFYELQDAY
HHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MEKDGSAKSSDNSLIEKEGFISQSLSKDGIPNQSLPDDHIGIENISVEPNKLYEAVSFLR
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NYGFNYLQCQGGYDEGPGKNLVSFYHFITVDDLQKIEKIKEVRLKVFLKRDSDLSIPSLY
HCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHH
KIFKGSDWQERETFDMYGINFIDHPNPKRLLMPEDWRGWPLRKDYIQPDFYELQDAY
HHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA