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The map label for this gene is purR [H]

Identifier: 126455315

GI number: 126455315

Start: 1393356

End: 1394372

Strand: Reverse

Name: purR [H]

Synonym: BURPS1106A_1420

Alternate gene names: 126455315

Gene position: 1394372-1393356 (Counterclockwise)

Preceding gene: 126451599

Following gene: 126454396

Centisome position: 34.96

GC content: 71.98

Gene sequence:

>1017_bases
ATGACGCCCACCATCAAAGACGTCGCCGCGCTCGCCGGCTTTTCGATCGCCACCGTGTCGCGCGCGATCAACGCGCCGCA
CACCGTCCATCCGGCGACGCTCGAAAAGATCCGCGCGGCGATCGGCGCGCTGCGCTTTCGCCCGAATCCGCTCGGCCGGC
AATTGCGCAGCGACCGCACGCAATTGATCGGCGTCGTGCTGCCGACGCTCGCGAATCCCGTGTTCGCCGAATGCCTGCAG
GGCGTCGACGAACTCGCGACGCAGGCCGGCTTCAAGCTGATCGTGATGTCGACCGAATACGATGCGGCGCGCGAGCGCCA
TGCGATCGAGACACTGCGCGCGCAGCGCGTGGAAGGGCTGATGCTCACCGTCGCCGACGCCGACGCGCACCCGCTGCTCG
ACGAGCTCGACCGCGACGGCCCGCTCTACGTGCTGATGCACAACGACACGCCGCATCGCCCGTCGGTGGCGGTCGACAAT
CGCCGCGCCGCGTACGACGGCGTGCGGATGCTGATCGAGCGCGGCCATCGGCGCGTGCTGATGCTCGCGGGCTCGCTCGA
CGCATCCGATCGCGCGCGGCTGCGCGTGCACGGCTATGCGCAGGCACTCGACGAGCGCGGGCTCGAACCGCTGCCCGCGC
TCGAGCTCGACTTCAATGCACCCGCGCTGCCGCACGCGATGCTCGCGCATCTGAGCGCGCGCGCGACGCGCCCCACCGCG
CTCTTCTGCAGCAACGACTGGCTCGCGATGGTCGTGATTCGCGGGCTGCGCGACGCGCACCTCGCGGTGCCCGACGACAT
GTCGGTGCTCGGCTTCGACGGCCTCGCGGTCGGCGAGCTGCTCGCGCCGCCGCTCGCGAGCGTCGCGACGCCGAATCGCG
AGATCGGCCGCGCCGCGTGGCGGCGCCTTGCCGAGCGCATCGCCGGCAAGCGCCATGCGCAACCCGCGCTGACGCTGCCG
CACGCGGTGCGCGACGGCGCGACCGTCGCGCCGCCGCGCGATGCGCGCATCGCCTGA

Upstream 100 bases:

>100_bases
CAGCCGCGCCCGCTCGCCTTCGCCGCCGTCATCAGTTCTTCATCGACGCCGCCGATAATCCCGTCGACGAAAACGTTTAC
ACGCCTCCGCTTCCCGCTTC

Downstream 100 bases:

>100_bases
ACCGTGCTTTCGCTTTCCGTCGCTTTCCATTCGCTTCGCCCTCGAGGAGACCTGCCGTGACCGTCCGCCGTTCGCTTCTT
CCGCGCTCGCCGCTCGCCGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQ
GVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGLMLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDN
RRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGKRHAQPALTLP
HAVRDGATVAPPRDARIA

Sequences:

>Translated_338_residues
MTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQ
GVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGLMLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDN
RRAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGKRHAQPALTLP
HAVRDGATVAPPRDARIA
>Mature_337_residues
TPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRTQLIGVVLPTLANPVFAECLQG
VDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGLMLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDNR
RAAYDGVRMLIERGHRRVLMLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTAL
FCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAWRRLAERIAGKRHAQPALTLPH
AVRDGATVAPPRDARIA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=30.8681672025724, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790194, Length=321, Percent_Identity=33.6448598130841, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1790715, Length=344, Percent_Identity=27.3255813953488, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=30.3951367781155, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787948, Length=346, Percent_Identity=30.9248554913295, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788474, Length=296, Percent_Identity=32.0945945945946, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=31.864406779661, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=29.5522388059701, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=27.9503105590062, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=26.8072289156627, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786268, Length=328, Percent_Identity=24.6951219512195, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790689, Length=313, Percent_Identity=22.3642172523962, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36660; Mature: 36529

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRT
CCCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QLIGVVLPTLANPVFAECLQGVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCE
MLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDNRRAAYDGVRMLIERGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEE
MLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
EEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEE
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAW
EEECCCHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHCCCHHHHCCCCHHHHHHHH
RRLAERIAGKRHAQPALTLPHAVRDGATVAPPRDARIA
HHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPTIKDVAALAGFSIATVSRAINAPHTVHPATLEKIRAAIGALRFRPNPLGRQLRSDRT
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QLIGVVLPTLANPVFAECLQGVDELATQAGFKLIVMSTEYDAARERHAIETLRAQRVEGL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCE
MLTVADADAHPLLDELDRDGPLYVLMHNDTPHRPSVAVDNRRAAYDGVRMLIERGHRRVL
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEE
MLAGSLDASDRARLRVHGYAQALDERGLEPLPALELDFNAPALPHAMLAHLSARATRPTA
EEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEE
LFCSNDWLAMVVIRGLRDAHLAVPDDMSVLGFDGLAVGELLAPPLASVATPNREIGRAAW
EEECCCHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHCCCHHHHCCCCHHHHHHHH
RRLAERIAGKRHAQPALTLPHAVRDGATVAPPRDARIA
HHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA