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The map label for this gene is dhbF [H]

Identifier: 126454659

GI number: 126454659

Start: 1971924

End: 1973801

Strand: Reverse

Name: dhbF [H]

Synonym: BURPS1106A_2010

Alternate gene names: 126454659

Gene position: 1973801-1971924 (Counterclockwise)

Preceding gene: 126453352

Following gene: 126453949

Centisome position: 49.49

GC content: 70.55

Gene sequence:

>1878_bases
ATGCATGTTTCGACCGATACGTCCGCCGAAGCCCATTCACCGGCGCCGAATTCACTCGATATGTCAGACAGTGACGCACT
GCGCCGGATTGCCGAAGCCGTCGACGGCGGTGCGGCGAACATCGAGCGAATCGTTCCGCTCGCCCGTGCGCGCGAGCGTA
TGCCGACGCGGCCTCGGCTCGAGCGGTGCGGCGGCGGGCGAGTGACGGCGGCGCACATCACGCTCGACTCGCGTGCGCGT
CTCGATGCGTTGCTGCACGCATTGCAACGCGCGATCGACCAGAACGCGGACCTGCGAACGTGCATTTTGGGGGCGTGCCT
GCGGCGGCCGATGCAAGTCACGCTTCGCGAGGTTCGCCTGCGAGTGCACGCCGCGACGCTCGACCCCGACCTCGATCCCG
CCGCGCAGTTGGCCGCGCTGAGCACCGGGCCCGGCATGCGCATCGACATGCAACGCCCGCCGTGGGTGCTCGCGTGCATC
GCGCGCATTCCGGGCAGCGGGCAATGGCTGCTGCGGCTCGTGGCAGCCCCGATCGCGGCCGGATTCGACGCGCTCGACGC
GCTGCTTCGCGAGACGGTGATTCACGGCGACCGGGAGCCCGGGCCGGCGCCGTTTCACTGGACTGTGGAAACGGCTGTTG
AATCGTGCGGAGGCGAACCTGCGTCGTTGCCGACCGCGGGCGCGGTTTGGCCGTCGAACGACGTATCACGCGCTTGCGAT
CCGGATGCCGCGTCGTGCGTCGAGGCGCGCATCGCCGCGATCGCGTCCGATCTGCCGGGCGTCGTGCATGGCGGACCACG
AGACGATTTGCGCGCGCTCGGACGAACGCCGTTGCAGGCGCTTCGACTCGCGCGCCGTATCCGCGACGCACTGGGCGTGA
CCGTACCGGTCGAGTCGATCCTCGCGAGTCCGACCATCGTCGAGCTTGCCGGGTACGTCGAGCAATTGCGCTCGCGGGAC
GTCCGCGACGGCGCTGCGCCCGTGTCGATCGGCGAAAAACCGGCGGACGCGGATGCTCGGGCACAGGCGCAGGCGGATAC
GGATACGGCGCACACCGATTGCCTGATCGTCATTCAAGCAGGCGGCGCCGAACAAGCGCCGGTGTTCTGCATCCCGGGCG
CGGGGGGCAGCGTCGCGTCGTTCGTTGCGCTTGCGAGCATGCTGCGCGCCGACATACCGGTATACGGCTTGCAGCCTCGC
GGGCTGGACGGCCTGGGGCCGCCGGACCGGTCCGTCGAAGCGGCTGCGCGCCGGTACGCGCGGGCCATTCTGGATGCCGC
CCCGCCCGGGCCGCCGCGCATCGTCGGCCACTCGTTCGGCGGCTGGATCGCGCTCGAGACAGCGCGGCTGCTGGACGGCA
TGGGAGCGCGCTGCGCCCCGCTCGTCCTGCTCGATTCGAATCCGCCGCCCGCGTCACAGGCCTGGCGCGCGCCTTCCGAG
GCAGACATGCTGCGCACGCTCGTCGGCCTGCTCGAGCAGGCCGCGGGCGGCGCCCCATCCGGGATCGGCGACGAAGAAAT
CGCCCGTTGCGCGGCAGCGGGCGAGGATGCGCGGGATGCGCTCGTCCACGCCTGCATGGTGAGGACCGCCCTGCTGCCGC
CGCGCGCGCCGGTCGAAGCGGTGCGGCACCTGCGGCGGGTATTCGAAGCCCATTCGAGCACCCGCTACGCGCCGGGCGGC
CGATACGCGGGCGACGCAACGGTGATCGTCGCCAACGGCGATCGCGACGCGGGCGAGATGGTGCCGGCGTTCGGATGGGC
CGCGCTGATCGAGCGAGTCGAGGTGGCCGTGACGCCGGGCAATCACATGAGCATGCTCGCGGCGCCGTATGTTCGTCACG
TCGCGCTGACGATGAAGACGGTATGGCGCATGATCTGA

Upstream 100 bases:

>100_bases
CGCGTTCGAAATACGGCGGCGGCGCTTGGCACCCGGCGCCGTCCGATCGCAACATGGAATGCGCGCTACGCGACATCGAA
CCCCGGGAGACCTGACCGCC

Downstream 100 bases:

>100_bases
ATCAACACTGCCGCCGCCGCGCTGCGATGGCCGTTCGTTGCGCCCCGTGCGCCGACCGATGAAGCCGGCCGGGCGCCGGC
ACGGCGGCGATCGGTCGTGG

Product: putative non-ribosomal peptide synthase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MHVSTDTSAEAHSPAPNSLDMSDSDALRRIAEAVDGGAANIERIVPLARARERMPTRPRLERCGGGRVTAAHITLDSRAR
LDALLHALQRAIDQNADLRTCILGACLRRPMQVTLREVRLRVHAATLDPDLDPAAQLAALSTGPGMRIDMQRPPWVLACI
ARIPGSGQWLLRLVAAPIAAGFDALDALLRETVIHGDREPGPAPFHWTVETAVESCGGEPASLPTAGAVWPSNDVSRACD
PDAASCVEARIAAIASDLPGVVHGGPRDDLRALGRTPLQALRLARRIRDALGVTVPVESILASPTIVELAGYVEQLRSRD
VRDGAAPVSIGEKPADADARAQAQADTDTAHTDCLIVIQAGGAEQAPVFCIPGAGGSVASFVALASMLRADIPVYGLQPR
GLDGLGPPDRSVEAAARRYARAILDAAPPGPPRIVGHSFGGWIALETARLLDGMGARCAPLVLLDSNPPPASQAWRAPSE
ADMLRTLVGLLEQAAGGAPSGIGDEEIARCAAAGEDARDALVHACMVRTALLPPRAPVEAVRHLRRVFEAHSSTRYAPGG
RYAGDATVIVANGDRDAGEMVPAFGWAALIERVEVAVTPGNHMSMLAAPYVRHVALTMKTVWRMI

Sequences:

>Translated_625_residues
MHVSTDTSAEAHSPAPNSLDMSDSDALRRIAEAVDGGAANIERIVPLARARERMPTRPRLERCGGGRVTAAHITLDSRAR
LDALLHALQRAIDQNADLRTCILGACLRRPMQVTLREVRLRVHAATLDPDLDPAAQLAALSTGPGMRIDMQRPPWVLACI
ARIPGSGQWLLRLVAAPIAAGFDALDALLRETVIHGDREPGPAPFHWTVETAVESCGGEPASLPTAGAVWPSNDVSRACD
PDAASCVEARIAAIASDLPGVVHGGPRDDLRALGRTPLQALRLARRIRDALGVTVPVESILASPTIVELAGYVEQLRSRD
VRDGAAPVSIGEKPADADARAQAQADTDTAHTDCLIVIQAGGAEQAPVFCIPGAGGSVASFVALASMLRADIPVYGLQPR
GLDGLGPPDRSVEAAARRYARAILDAAPPGPPRIVGHSFGGWIALETARLLDGMGARCAPLVLLDSNPPPASQAWRAPSE
ADMLRTLVGLLEQAAGGAPSGIGDEEIARCAAAGEDARDALVHACMVRTALLPPRAPVEAVRHLRRVFEAHSSTRYAPGG
RYAGDATVIVANGDRDAGEMVPAFGWAALIERVEVAVTPGNHMSMLAAPYVRHVALTMKTVWRMI
>Mature_625_residues
MHVSTDTSAEAHSPAPNSLDMSDSDALRRIAEAVDGGAANIERIVPLARARERMPTRPRLERCGGGRVTAAHITLDSRAR
LDALLHALQRAIDQNADLRTCILGACLRRPMQVTLREVRLRVHAATLDPDLDPAAQLAALSTGPGMRIDMQRPPWVLACI
ARIPGSGQWLLRLVAAPIAAGFDALDALLRETVIHGDREPGPAPFHWTVETAVESCGGEPASLPTAGAVWPSNDVSRACD
PDAASCVEARIAAIASDLPGVVHGGPRDDLRALGRTPLQALRLARRIRDALGVTVPVESILASPTIVELAGYVEQLRSRD
VRDGAAPVSIGEKPADADARAQAQADTDTAHTDCLIVIQAGGAEQAPVFCIPGAGGSVASFVALASMLRADIPVYGLQPR
GLDGLGPPDRSVEAAARRYARAILDAAPPGPPRIVGHSFGGWIALETARLLDGMGARCAPLVLLDSNPPPASQAWRAPSE
ADMLRTLVGLLEQAAGGAPSGIGDEEIARCAAAGEDARDALVHACMVRTALLPPRAPVEAVRHLRRVFEAHSSTRYAPGG
RYAGDATVIVANGDRDAGEMVPAFGWAALIERVEVAVTPGNHMSMLAAPYVRHVALTMKTVWRMI

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG3319

COG function: function code Q; Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 65793; Mature: 65793

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVSTDTSAEAHSPAPNSLDMSDSDALRRIAEAVDGGAANIERIVPLARARERMPTRPRL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
ERCGGGRVTAAHITLDSRARLDALLHALQRAIDQNADLRTCILGACLRRPMQVTLREVRL
HHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHH
RVHAATLDPDLDPAAQLAALSTGPGMRIDMQRPPWVLACIARIPGSGQWLLRLVAAPIAA
HHEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHC
GFDALDALLRETVIHGDREPGPAPFHWTVETAVESCGGEPASLPTAGAVWPSNDVSRACD
CHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PDAASCVEARIAAIASDLPGVVHGGPRDDLRALGRTPLQALRLARRIRDALGVTVPVESI
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
LASPTIVELAGYVEQLRSRDVRDGAAPVSIGEKPADADARAQAQADTDTAHTDCLIVIQA
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEC
GGAEQAPVFCIPGAGGSVASFVALASMLRADIPVYGLQPRGLDGLGPPDRSVEAAARRYA
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
RAILDAAPPGPPRIVGHSFGGWIALETARLLDGMGARCAPLVLLDSNPPPASQAWRAPSE
HHHHHCCCCCCCCEECCCCCCEEEHHHHHHHCCCCCCCEEEEEECCCCCCCHHHCCCCCH
ADMLRTLVGLLEQAAGGAPSGIGDEEIARCAAAGEDARDALVHACMVRTALLPPRAPVEA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHH
VRHLRRVFEAHSSTRYAPGGRYAGDATVIVANGDRDAGEMVPAFGWAALIERVEVAVTPG
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCC
NHMSMLAAPYVRHVALTMKTVWRMI
CCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVSTDTSAEAHSPAPNSLDMSDSDALRRIAEAVDGGAANIERIVPLARARERMPTRPRL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
ERCGGGRVTAAHITLDSRARLDALLHALQRAIDQNADLRTCILGACLRRPMQVTLREVRL
HHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHH
RVHAATLDPDLDPAAQLAALSTGPGMRIDMQRPPWVLACIARIPGSGQWLLRLVAAPIAA
HHEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHC
GFDALDALLRETVIHGDREPGPAPFHWTVETAVESCGGEPASLPTAGAVWPSNDVSRACD
CHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
PDAASCVEARIAAIASDLPGVVHGGPRDDLRALGRTPLQALRLARRIRDALGVTVPVESI
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
LASPTIVELAGYVEQLRSRDVRDGAAPVSIGEKPADADARAQAQADTDTAHTDCLIVIQA
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEC
GGAEQAPVFCIPGAGGSVASFVALASMLRADIPVYGLQPRGLDGLGPPDRSVEAAARRYA
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
RAILDAAPPGPPRIVGHSFGGWIALETARLLDGMGARCAPLVLLDSNPPPASQAWRAPSE
HHHHHCCCCCCCCEECCCCCCEEEHHHHHHHCCCCCCCEEEEEECCCCCCCHHHCCCCCH
ADMLRTLVGLLEQAAGGAPSGIGDEEIARCAAAGEDARDALVHACMVRTALLPPRAPVEA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHH
VRHLRRVFEAHSSTRYAPGGRYAGDATVIVANGDRDAGEMVPAFGWAALIERVEVAVTPG
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCC
NHMSMLAAPYVRHVALTMKTVWRMI
CCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]