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The map label for this gene is rph

Identifier: 126454192

GI number: 126454192

Start: 2952954

End: 2953685

Strand: Direct

Name: rph

Synonym: BURPS1106A_3017

Alternate gene names: 126454192

Gene position: 2952954-2953685 (Clockwise)

Preceding gene: 126454031

Following gene: 126455253

Centisome position: 74.04

GC content: 70.08

Gene sequence:

>732_bases
ATGACGAATTCCTCCCTACGCCCGAGCGGGCGCCGCGCCGACCAGTTGCGCGACGTGCGCATCACGCGCCACTACACGAA
GCATGCGGAAGGCGCGGTGCTCGTCGAGTTCGGCGACACGAAAGTGATCTGCACGGCGAGCGTCGCCGAGCGCGTGCCCG
AATTCCTGCGCGAGCGCGGCCAAGGCTGGCTCACCGCCGAATACGGGATGCTGCCGCGTGCGACGCACACGCGCAGCGAC
CGCGAAGCGGCGCGCGGCAAGCAAACCGGCCGAACGCAGGAAATTCAGCGCCTGATCGGCCGCGCGCTGCGCGCGGTATT
CGATCTGAACGCGCTCGGCCCACGCACGCTGCACCTCGATTGCGACGTGATCCAGGCCGACGGCGGCACGCGCACGGCGA
GCATCACGGGCGCGTTCGTCGCCGCGCACGACGCGGTGACGAAACTCGTCGCGGCCGGCAGGATCGCGCGCTCGCCGATC
ACCGATTACGTCGCGGCAATCTCGGTCGGCGTGTTCGGCGGCACGCCGGTGCTCGACCTCGACTACGACGAAGACTCCGC
GTGCGACACCGACATGAACGTCGTGATGACGGGCGCGGGCGGCTTCGTCGAGGTGCAGGGCACCGCGGAAGGCGCGCCGT
TCTCGCGCACCGAGATGAACGCGCTTCTCGATCTCGCGCAGGCCGGCATCGGCGAGCTCGTGCGGCTGCAGCGCGCGGCG
CTGGAGGCATGA

Upstream 100 bases:

>100_bases
GCGCGAAACATCCATTATCCCATTTTTGCGGGCCGGACCCGCCGGGCCGATGCGCCGCGCGCGCGGTAAAATTCGGGTTT
CTCCTCAGCCTATTGTCCCG

Downstream 100 bases:

>100_bases
CGATGTCGCCCGCATCGCCCGACGCCGCGCGCTCGCGCATCGTCCTCGCCTCGAACAATCCCGGCAAGCTGCGCGAGTTC
GCCGCGCTCTTCTCGACGGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPI
TDYVAAISVGVFGGTPVLDLDYDEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
LEA

Sequences:

>Translated_243_residues
MTNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPI
TDYVAAISVGVFGGTPVLDLDYDEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
LEA
>Mature_242_residues
TNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERGQGWLTAEYGMLPRATHTRSDR
EAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPIT
DYVAAISVGVFGGTPVLDLDYDEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAAL
EA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=67.1428571428571, Blast_Score=290, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI71981632, Length=191, Percent_Identity=26.1780104712042, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_BURM7 (A3MML4)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001081500.1
- ProteinModelPortal:   A3MML4
- SMR:   A3MML4
- GeneID:   4894920
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_1966
- TIGR:   BMA10247_1966
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   BMAL320389:BMA10247_1966-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26053; Mature: 25922

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLD
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPITDYVAAISVGVFGGTPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEE
DYDEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
CCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEA
HCC
>Mature Secondary Structure 
TNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLD
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPITDYVAAISVGVFGGTPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEE
DYDEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
CCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA