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The map label for this gene is lcfA [H]

Identifier: 126453478

GI number: 126453478

Start: 1962231

End: 1964207

Strand: Reverse

Name: lcfA [H]

Synonym: BURPS1106A_1999

Alternate gene names: 126453478

Gene position: 1964207-1962231 (Counterclockwise)

Preceding gene: 126452247

Following gene: 126454631

Centisome position: 49.25

GC content: 72.94

Gene sequence:

>1977_bases
ATGGCGGTTCGCGACATCCACGACATCGAGCAGATCGAGCGCGTGCCGCTGCACGCGCGCGCGCTGCCCGCCAACACGCT
GCAGGTTTTCGACGAGCGCGCGGCGAAGACGCCCGACGCGCCCGCCCTCACCTTCTTTCTCGACGCCGGCCGGCTCGACC
GCTCGCATACGTGGACCTTCGCCGAGCTGCGCGCCGACATCGTCAGGACGGCGAACGTGCTCGCGAGCGTCGGCATCGGC
GCGGGCGACGTCGCCGCGTTCGTGCTGCCGAACCTGCCCGACACGCATTTCGCGATCTGGGGCGGCGAGGCGGCCGGCAT
CGCGATGGCGATCAACCCGCTGCTCGACGGCGCGCAGATCGCGGAGCTCGTCGACGCGGCGCGCGCCAAGGTGCTGATTT
GCGTCGCGCCGACGCCGGGCGTCGACATCTGGCCGAAGCTCGCGCCGCACCTGGACGCGATGCCGACTGTCGAGACCGTG
ATCTGGGTCGATCTGCGGCCCTACGTGTCATTGCCCAAGCGCGCGGCGCTCGCGTGGATCGAGCGGCGCGAGAAGGCGCG
CCTGCGCGGCGCGCGGGTGCGCATCGTCAATCTGCACGCCGAGATGCGCCGGCAGCCGGGCGACAGGCTGATCCGGCCGC
GCGCGATCGGGCCCGACGAGCCGTCGTCGTACTTCTGCACGGGCGGCACGACGGGCCGCCCGAAGATCGCGGTGCGCACG
CACGGCTCCGAGGTGTTCGACGTGTGGGCGGCGAGCGAGACGCAGGCGCGCGACGACGAGGGCGCGCGCACGGTGTTCTG
CGGGCTGCCGCTCTTTCACGTGAACGGCCAGCTCGTCACCGGCCTGATGGCGTGGCTGCGCGGCCATCATGTCGTGCTCG
GCACGCCGCAGGGCTACCGCGGCAAGAACGTGATCGCGCGCTTCTGGGCGATCGTCGAGGCTTATCGGATCAATGCGTTT
TCCGGCGTGCCGACGCTTTTCGCCGCGCTGTTGCAGCAGCCTGTCGGCCGGCACGACATCGGCTCGCTCGAATACGCGGC
GTGCGGCGCGGCGCCGATGCCCGTCGAGCTCGCGCGCAGCTTCGAGCGCACGACCGGCGTGAAGATCGTCGAGGGCTACG
GGCTGACCGAGAGCGCGTGCATCGCGTCGCTCAACCCGCTCGACGGCGAGCGGCGCATCGGCTCGATCGGCCTGCGCCTG
CCCTACCAGCGCATGCGCGCGGTGATCGTCGACGACACCGGGCGCTACGTGCGCGACGCGCTCGTCGACGAAGTCGGGCT
GATCGCGCTCGCGGGGCCGAACGTGTTTCGCGGCTACCTCGATCCGGCGCACGAGCGCGGCTTGTGGATCGACATCGCGG
GCGAGCGGTGGCTGAACACGGGCGACCTCGGCCGGCGCGACGCGCACGGCTATTTCTGGCTCGTCGGCCGCAAGAAGGAG
CTGATCATCCGCGGCGGGCACAACATCGATCCGCGGATCATCGAGGACGCGCTCGCCGCCCATCCGGCCGTCGCGCTCGC
CGCGGCGATCGGCCGCCCGGACGCGCACGCGGGCGAGCTGCCCGTCGCGTACGTCCAGTTGAAGGCGGGCGCGAGCGCCG
ACGAGGCCGCGCTGCTCGCGTTCGCCGCGGACGCGATTGCCGAGCGCGCGGCGGTGCCCAAGCACGTGCGGATTCTCGAG
GCGGTGCCGACCACCGCGGTGGGCAAGATCTTCAAGCCGCAGTTGCAGCGGCTCGAGATCGCCGACGTCGTCGCCGCGTG
CGCGCGCGACGCGCAGGTCGCGCTCGAGCGGGTGGACGTCGTGCAGGACGCGCGGCGCGGGCTCGTCGCGCAGGTCGCCG
TGCGCGGCGCACGCGAGGCGCTCGCCGAGCGGCTCGCCCGTTACGCGTTCCCCGTCGACTGGAGCGGCGACGGCGCGCAC
GCGCGGGCCTCCGAGGCGCAGCGCGACGCGGCCGCCGCGCGCGAGCGCGCGTCGTGA

Upstream 100 bases:

>100_bases
GCGCCCCGGCACCGCCCGAGCGGGCCGCCGCCGCGCTGTGCCCGCCCGGCCGAGGCCCGCATCCGATAGACGACTTTCGC
AGTCCGACAGGAGTTCATGC

Downstream 100 bases:

>100_bases
ACGCCGCGCCGCGCTGCCCGCCGCCGCCCCGCCGGGTTCGGCGGGGCGGCGCCATGCGGGGCGAACCCTGATGCGCGCCG
GCGACTTTCGCGTGACCGCG

Product: AMP-binding domain protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MAVRDIHDIEQIERVPLHARALPANTLQVFDERAAKTPDAPALTFFLDAGRLDRSHTWTFAELRADIVRTANVLASVGIG
AGDVAAFVLPNLPDTHFAIWGGEAAGIAMAINPLLDGAQIAELVDAARAKVLICVAPTPGVDIWPKLAPHLDAMPTVETV
IWVDLRPYVSLPKRAALAWIERREKARLRGARVRIVNLHAEMRRQPGDRLIRPRAIGPDEPSSYFCTGGTTGRPKIAVRT
HGSEVFDVWAASETQARDDEGARTVFCGLPLFHVNGQLVTGLMAWLRGHHVVLGTPQGYRGKNVIARFWAIVEAYRINAF
SGVPTLFAALLQQPVGRHDIGSLEYAACGAAPMPVELARSFERTTGVKIVEGYGLTESACIASLNPLDGERRIGSIGLRL
PYQRMRAVIVDDTGRYVRDALVDEVGLIALAGPNVFRGYLDPAHERGLWIDIAGERWLNTGDLGRRDAHGYFWLVGRKKE
LIIRGGHNIDPRIIEDALAAHPAVALAAAIGRPDAHAGELPVAYVQLKAGASADEAALLAFAADAIAERAAVPKHVRILE
AVPTTAVGKIFKPQLQRLEIADVVAACARDAQVALERVDVVQDARRGLVAQVAVRGAREALAERLARYAFPVDWSGDGAH
ARASEAQRDAAAARERAS

Sequences:

>Translated_658_residues
MAVRDIHDIEQIERVPLHARALPANTLQVFDERAAKTPDAPALTFFLDAGRLDRSHTWTFAELRADIVRTANVLASVGIG
AGDVAAFVLPNLPDTHFAIWGGEAAGIAMAINPLLDGAQIAELVDAARAKVLICVAPTPGVDIWPKLAPHLDAMPTVETV
IWVDLRPYVSLPKRAALAWIERREKARLRGARVRIVNLHAEMRRQPGDRLIRPRAIGPDEPSSYFCTGGTTGRPKIAVRT
HGSEVFDVWAASETQARDDEGARTVFCGLPLFHVNGQLVTGLMAWLRGHHVVLGTPQGYRGKNVIARFWAIVEAYRINAF
SGVPTLFAALLQQPVGRHDIGSLEYAACGAAPMPVELARSFERTTGVKIVEGYGLTESACIASLNPLDGERRIGSIGLRL
PYQRMRAVIVDDTGRYVRDALVDEVGLIALAGPNVFRGYLDPAHERGLWIDIAGERWLNTGDLGRRDAHGYFWLVGRKKE
LIIRGGHNIDPRIIEDALAAHPAVALAAAIGRPDAHAGELPVAYVQLKAGASADEAALLAFAADAIAERAAVPKHVRILE
AVPTTAVGKIFKPQLQRLEIADVVAACARDAQVALERVDVVQDARRGLVAQVAVRGAREALAERLARYAFPVDWSGDGAH
ARASEAQRDAAAARERAS
>Mature_657_residues
AVRDIHDIEQIERVPLHARALPANTLQVFDERAAKTPDAPALTFFLDAGRLDRSHTWTFAELRADIVRTANVLASVGIGA
GDVAAFVLPNLPDTHFAIWGGEAAGIAMAINPLLDGAQIAELVDAARAKVLICVAPTPGVDIWPKLAPHLDAMPTVETVI
WVDLRPYVSLPKRAALAWIERREKARLRGARVRIVNLHAEMRRQPGDRLIRPRAIGPDEPSSYFCTGGTTGRPKIAVRTH
GSEVFDVWAASETQARDDEGARTVFCGLPLFHVNGQLVTGLMAWLRGHHVVLGTPQGYRGKNVIARFWAIVEAYRINAFS
GVPTLFAALLQQPVGRHDIGSLEYAACGAAPMPVELARSFERTTGVKIVEGYGLTESACIASLNPLDGERRIGSIGLRLP
YQRMRAVIVDDTGRYVRDALVDEVGLIALAGPNVFRGYLDPAHERGLWIDIAGERWLNTGDLGRRDAHGYFWLVGRKKEL
IIRGGHNIDPRIIEDALAAHPAVALAAAIGRPDAHAGELPVAYVQLKAGASADEAALLAFAADAIAERAAVPKHVRILEA
VPTTAVGKIFKPQLQRLEIADVVAACARDAQVALERVDVVQDARRGLVAQVAVRGAREALAERLARYAFPVDWSGDGAHA
RASEAQRDAAAARERAS

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=572, Percent_Identity=24.8251748251748, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI122937307, Length=592, Percent_Identity=25.6756756756757, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI38505220, Length=560, Percent_Identity=26.6071428571429, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI42544132, Length=570, Percent_Identity=23.3333333333333, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI187761345, Length=387, Percent_Identity=29.1989664082687, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761343, Length=387, Percent_Identity=29.1989664082687, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI115511026, Length=573, Percent_Identity=23.2111692844677, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI28416953, Length=618, Percent_Identity=24.2718446601942, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI58082049, Length=569, Percent_Identity=24.2530755711775, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI157311624, Length=563, Percent_Identity=23.9786856127886, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI157311622, Length=563, Percent_Identity=23.9786856127886, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI27477105, Length=557, Percent_Identity=25.3141831238779, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=567, Percent_Identity=21.5167548500882, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=560, Percent_Identity=28.3928571428571, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI221142682, Length=557, Percent_Identity=25.1346499102334, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI145693145, Length=371, Percent_Identity=31.8059299191375, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1786529, Length=586, Percent_Identity=24.5733788395904, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786810, Length=370, Percent_Identity=28.1081081081081, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789201, Length=363, Percent_Identity=27.5482093663912, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=23.1329690346084, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786801, Length=559, Percent_Identity=23.7924865831843, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=533, Percent_Identity=28.1425891181989, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17558820, Length=361, Percent_Identity=30.4709141274238, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=387, Percent_Identity=28.9405684754522, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=367, Percent_Identity=28.6103542234332, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=376, Percent_Identity=27.1276595744681, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=376, Percent_Identity=27.1276595744681, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=376, Percent_Identity=27.1276595744681, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=581, Percent_Identity=27.0223752151463, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=26.0162601626016, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=210, Percent_Identity=30, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=389, Percent_Identity=24.6786632390745, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=394, Percent_Identity=28.1725888324873, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=584, Percent_Identity=23.8013698630137, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=584, Percent_Identity=23.8013698630137, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=371, Percent_Identity=23.989218328841, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17510401, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=366, Percent_Identity=29.7814207650273, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=601, Percent_Identity=23.6272878535774, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=592, Percent_Identity=22.4662162162162, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=28.2485875706215, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355181, Length=532, Percent_Identity=26.1278195488722, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=25.4752851711027, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648260, Length=362, Percent_Identity=24.8618784530387, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653035, Length=374, Percent_Identity=24.8663101604278, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 71173; Mature: 71042

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRDIHDIEQIERVPLHARALPANTLQVFDERAAKTPDAPALTFFLDAGRLDRSHTWTF
CCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEH
AELRADIVRTANVLASVGIGAGDVAAFVLPNLPDTHFAIWGGEAAGIAMAINPLLDGAQI
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEEEHHHCCHHHH
AELVDAARAKVLICVAPTPGVDIWPKLAPHLDAMPTVETVIWVDLRPYVSLPKRAALAWI
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHCCCHHHHHHHH
ERREKARLRGARVRIVNLHAEMRRQPGDRLIRPRAIGPDEPSSYFCTGGTTGRPKIAVRT
HHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEE
HGSEVFDVWAASETQARDDEGARTVFCGLPLFHVNGQLVTGLMAWLRGHHVVLGTPQGYR
CCCEEEEEEECCCCCCCCCCCCEEEEECCEEEEECCHHHHHHHHHHCCCEEEEECCCCCC
GKNVIARFWAIVEAYRINAFSGVPTLFAALLQQPVGRHDIGSLEYAACGAAPMPVELARS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHHHHH
FERTTGVKIVEGYGLTESACIASLNPLDGERRIGSIGLRLPYQRMRAVIVDDTGRYVRDA
HHHHCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCHHHHHH
LVDEVGLIALAGPNVFRGYLDPAHERGLWIDIAGERWLNTGDLGRRDAHGYFWLVGRKKE
HHHHCCEEEEECCHHHHHCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCEE
LIIRGGHNIDPRIIEDALAAHPAVALAAAIGRPDAHAGELPVAYVQLKAGASADEAALLA
EEEECCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHH
FAADAIAERAAVPKHVRILEAVPTTAVGKIFKPQLQRLEIADVVAACARDAQVALERVDV
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQDARRGLVAQVAVRGAREALAERLARYAFPVDWSGDGAHARASEAQRDAAAARERAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVRDIHDIEQIERVPLHARALPANTLQVFDERAAKTPDAPALTFFLDAGRLDRSHTWTF
CCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEH
AELRADIVRTANVLASVGIGAGDVAAFVLPNLPDTHFAIWGGEAAGIAMAINPLLDGAQI
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEEEHHHCCHHHH
AELVDAARAKVLICVAPTPGVDIWPKLAPHLDAMPTVETVIWVDLRPYVSLPKRAALAWI
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHCCCHHHHHHHH
ERREKARLRGARVRIVNLHAEMRRQPGDRLIRPRAIGPDEPSSYFCTGGTTGRPKIAVRT
HHHHHHHHCCCEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEE
HGSEVFDVWAASETQARDDEGARTVFCGLPLFHVNGQLVTGLMAWLRGHHVVLGTPQGYR
CCCEEEEEEECCCCCCCCCCCCEEEEECCEEEEECCHHHHHHHHHHCCCEEEEECCCCCC
GKNVIARFWAIVEAYRINAFSGVPTLFAALLQQPVGRHDIGSLEYAACGAAPMPVELARS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHHHHH
FERTTGVKIVEGYGLTESACIASLNPLDGERRIGSIGLRLPYQRMRAVIVDDTGRYVRDA
HHHHCCCEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCHHHHHH
LVDEVGLIALAGPNVFRGYLDPAHERGLWIDIAGERWLNTGDLGRRDAHGYFWLVGRKKE
HHHHCCEEEEECCHHHHHCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCEE
LIIRGGHNIDPRIIEDALAAHPAVALAAAIGRPDAHAGELPVAYVQLKAGASADEAALLA
EEEECCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHH
FAADAIAERAAVPKHVRILEAVPTTAVGKIFKPQLQRLEIADVVAACARDAQVALERVDV
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQDARRGLVAQVAVRGAREALAERLARYAFPVDWSGDGAHARASEAQRDAAAARERAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]