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The map label for this gene is panB

Identifier: 126453190

GI number: 126453190

Start: 3224454

End: 3225269

Strand: Direct

Name: panB

Synonym: BURPS1106A_3307

Alternate gene names: 126453190

Gene position: 3224454-3225269 (Clockwise)

Preceding gene: 126455460

Following gene: 126453268

Centisome position: 80.84

GC content: 69.36

Gene sequence:

>816_bases
ATGACCTACCTTCAGGAATCCAGCCGACCGGCCGTGACGGTACCCAAGCTGCAGGCGATGCGGGAAGCCGGCGAGAAGAT
CGCGATGCTCACCAGCTACGACGCAAGCTTCGCCGCCCTCCTCGATCGCGCGAACGTCGACGTGCAACTGATCGGCGATT
CGCTCGGCAACGTGCTGCAGGGCCAGGCGACGACGCTGCCCGTCACGCTCGACGACATCGCGTATCACACCGCATGCGTC
GCGCGCGCGCAGCCGCGCGGGCTCGTCGTCGCGGACCTGCCGTTCGGCACCTACGGCACGCCCGCCGACGCATTCGCGAG
CGCGGTGAAGCTGATGCGCGCGGGCGCGCAGATGGTCAAGCTCGAAGGCGGCGAATGGCTCGCCGAAACGGTGCGCTTCC
TCGTCGAGCGGGCCGTGCCCGTGTGCGCGCACGTCGGCCTCACGCCGCAGTCGGTGCACGCGTTCGGCGGCTTCAAGGTG
CAAGGCAAGACGGAAGCAGGCGCCGCGCAATTGCTGCGCGATGCGCGCGCGGTCGAGGAAGCCGGCGCGCAGCTCATCGT
GCTCGAGGCGGTGCCCACGCTCGTCGCGGCCGAAGTCACGCGCGAGCTGTCGATCCCGACGATCGGCATCGGCGCGGGCG
CCGAATGCTCGGGCCAGGTACTCGTGCTGCACGACATGCTCGGCGTGTTCCCCGGCAAGCGGCCGCGCTTCGTCAAGGAT
TTCATGCAGGGGCAGCCGAGCATCTTCGCAGCGGTCGAGGCCTACGTGCGCGCGGTGAAGGACGGCAGCTTCCCCGGGCC
CGAGCATTCGTTCTGA

Upstream 100 bases:

>100_bases
AAGGAATTCTTCGTCAAGGGCGGCAGCGCGCGCTGACGAACGCGCGCGGCGTCGCCGATCGCGACGCCGCCCTTTTCCTC
ACCATCGAATCGGACCTCCC

Downstream 100 bases:

>100_bases
CGGCACGGCCGACTCGGCCCCCCGGGCGCGCGCGGCCGCCCGCGGCATGCGGCGGCCCCGCCGCACTTCGCGAAGCTCGA
CAAAAAAAGCGGCATCCCGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTYLQESSRPAVTVPKLQAMREAGEKIAMLTSYDASFAALLDRANVDVQLIGDSLGNVLQGQATTLPVTLDDIAYHTACV
ARAQPRGLVVADLPFGTYGTPADAFASAVKLMRAGAQMVKLEGGEWLAETVRFLVERAVPVCAHVGLTPQSVHAFGGFKV
QGKTEAGAAQLLRDARAVEEAGAQLIVLEAVPTLVAAEVTRELSIPTIGIGAGAECSGQVLVLHDMLGVFPGKRPRFVKD
FMQGQPSIFAAVEAYVRAVKDGSFPGPEHSF

Sequences:

>Translated_271_residues
MTYLQESSRPAVTVPKLQAMREAGEKIAMLTSYDASFAALLDRANVDVQLIGDSLGNVLQGQATTLPVTLDDIAYHTACV
ARAQPRGLVVADLPFGTYGTPADAFASAVKLMRAGAQMVKLEGGEWLAETVRFLVERAVPVCAHVGLTPQSVHAFGGFKV
QGKTEAGAAQLLRDARAVEEAGAQLIVLEAVPTLVAAEVTRELSIPTIGIGAGAECSGQVLVLHDMLGVFPGKRPRFVKD
FMQGQPSIFAAVEAYVRAVKDGSFPGPEHSF
>Mature_270_residues
TYLQESSRPAVTVPKLQAMREAGEKIAMLTSYDASFAALLDRANVDVQLIGDSLGNVLQGQATTLPVTLDDIAYHTACVA
RAQPRGLVVADLPFGTYGTPADAFASAVKLMRAGAQMVKLEGGEWLAETVRFLVERAVPVCAHVGLTPQSVHAFGGFKVQ
GKTEAGAAQLLRDARAVEEAGAQLIVLEAVPTLVAAEVTRELSIPTIGIGAGAECSGQVLVLHDMLGVFPGKRPRFVKDF
MQGQPSIFAAVEAYVRAVKDGSFPGPEHSF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=259, Percent_Identity=54.0540540540541, Blast_Score=284, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6319653, Length=278, Percent_Identity=35.9712230215827, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BURM7 (A3MN95)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001081731.1
- ProteinModelPortal:   A3MN95
- SMR:   A3MN95
- GeneID:   4893045
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_2202
- TIGR:   BMA10247_2202
- HOGENOM:   HBG299908
- OMA:   AKFVRNF
- ProtClustDB:   PRK00311
- BioCyc:   BMAL320389:BMA10247_2202-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28618; Mature: 28487

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 92-92 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLQESSRPAVTVPKLQAMREAGEKIAMLTSYDASFAALLDRANVDVQLIGDSLGNVLQ
CCCCCCCCCCCEECHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCEEEEECHHHHHHHC
GQATTLPVTLDDIAYHTACVARAQPRGLVVADLPFGTYGTPADAFASAVKLMRAGAQMVK
CCCEEECEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
LEGGEWLAETVRFLVERAVPVCAHVGLTPQSVHAFGGFKVQGKTEAGAAQLLRDARAVEE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHH
AGAQLIVLEAVPTLVAAEVTRELSIPTIGIGAGAECSGQVLVLHDMLGVFPGKRPRFVKD
CCCCEEEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEHHHHHHHCCCCCCHHHHH
FMQGQPSIFAAVEAYVRAVKDGSFPGPEHSF
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TYLQESSRPAVTVPKLQAMREAGEKIAMLTSYDASFAALLDRANVDVQLIGDSLGNVLQ
CCCCCCCCCCEECHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCEEEEECHHHHHHHC
GQATTLPVTLDDIAYHTACVARAQPRGLVVADLPFGTYGTPADAFASAVKLMRAGAQMVK
CCCEEECEEHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
LEGGEWLAETVRFLVERAVPVCAHVGLTPQSVHAFGGFKVQGKTEAGAAQLLRDARAVEE
ECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHH
AGAQLIVLEAVPTLVAAEVTRELSIPTIGIGAGAECSGQVLVLHDMLGVFPGKRPRFVKD
CCCCEEEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEHHHHHHHCCCCCCHHHHH
FMQGQPSIFAAVEAYVRAVKDGSFPGPEHSF
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA