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The map label for this gene is lipA [H]

Identifier: 126453169

GI number: 126453169

Start: 427455

End: 428444

Strand: Direct

Name: lipA [H]

Synonym: BURPS1106A_0464

Alternate gene names: 126453169

Gene position: 427455-428444 (Clockwise)

Preceding gene: 126452030

Following gene: 126453063

Centisome position: 10.72

GC content: 65.56

Gene sequence:

>990_bases
ATGACTGACCTTACCGCTACCCCCGCACCCGCCGAACCGGCCGCTTCCGCCTACGATCCGACCGCCAAGCAGAAGGCGCA
GGCGAAGACCGCGCGCATTCCGATCAAGATCGTCCCGATCGAGAAGCTGAAGAAGCCCGAATGGATCCGCGTGAAGGCGG
CCACCGGCAGCTCGCGCTTCAACGAGATCAAGACGATCCTGCGCGAGCACAACCTGCATACGGTATGCGAGGAAGCGAGC
TGCCCGAACATCGGCGAATGCTTCGGCAAGGGCACCGCGACGTTCATGATCATGGGCGACAAGTGCACGCGCCGCTGCCC
GTTCTGCGACGTCGGCCACGGCCGGCCCGATCCGCTCGACGCAGACGAGCCGAAGAACCTCGCGCGCACGATCGCGGCGC
TCAAGCTCAAGTACGTGGTGATCACGAGCGTCGACCGCGACGATCTGCGCGACGGCGGCGCCGGCCACTTCGTCGAATGC
ATCCGCGAAGTGCGCGAGCAGTCGCCCGCGACGCGCATCGAGATCCTGACACCGGACTTCCGTGGCCGCCTCGACCGTGC
GCTCGCGATCCTGAACGCGGCGCCGCCCGACGTGATGAACCACAATCTCGAAACGGTGCCGCGCCTGTACAAGGAGGCGC
GCCCCGGCTCGGACTATGCGCATTCGCTGAAGCTCCTGAAGGATTTCAAGGCGCTGCATCCGGATGTCGCGACGAAATCG
GGCCTGATGGTCGGCCTGGGCGAGACGACCGACGAGATCCTGCAGGTGATGCGCGACCTGCGCGCGCACGACGTCGACAT
GCTGACGATCGGCCAATACCTGCAGCCGTCCGAGCACCATCTGCCGGTGCGCGAGTACGTGCATCCGGACACGTTCAAGA
TGTACGAGGAAGAGGCGTACAAGATGGGCTTCACGCACGCGGCCGTCGGCGCGATGGTGCGCTCGAGCTATCACGCCGAT
CTGCAGGCGCACGGCGCCGGAGTGGTTTGA

Upstream 100 bases:

>100_bases
TGGCACGAAGTCGCGCAGACGCTCGTGCGCCGCCTGATCGCCCACCTCGACGGCGCAACCGCGGCCGCCGCATTGCCGCA
GCAGGCACTGGAACAATCGA

Downstream 100 bases:

>100_bases
GCGGGATGGCGGGCTGACCGGCGGCCGGCCGCCGAATGAGGAAACCGCAGCCTTCGCTGCGGTTTTTTTATGCGCGCGGC
GCGCGAGGCGATGTGCGCCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTDLTATPAPAEPAASAYDPTAKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFNEIKTILREHNLHTVCEEAS
CPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAGHFVEC
IREVREQSPATRIEILTPDFRGRLDRALAILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATKS
GLMVGLGETTDEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVREYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHAD
LQAHGAGVV

Sequences:

>Translated_329_residues
MTDLTATPAPAEPAASAYDPTAKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFNEIKTILREHNLHTVCEEAS
CPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAGHFVEC
IREVREQSPATRIEILTPDFRGRLDRALAILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATKS
GLMVGLGETTDEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVREYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHAD
LQAHGAGVV
>Mature_328_residues
TDLTATPAPAEPAASAYDPTAKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRFNEIKTILREHNLHTVCEEASC
PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAGHFVECI
REVREQSPATRIEILTPDFRGRLDRALAILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATKSG
LMVGLGETTDEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVREYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHADL
QAHGAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=48.2517482517483, Blast_Score=276, Evalue=1e-74,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=48.8, Blast_Score=241, Evalue=8e-64,
Organism=Escherichia coli, GI1786846, Length=309, Percent_Identity=64.0776699029126, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=46.4566929133858, Blast_Score=230, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=289, Percent_Identity=44.9826989619377, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=46.8531468531469, Blast_Score=261, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36429; Mature: 36298

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTATPAPAEPAASAYDPTAKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRF
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHCCCCEEEEEECCCCHHH
NEIKTILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD
HHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCC
ADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAGHFVECIREVREQSPATRIEILTPDF
CCCHHHHHHHHHHHHHEEEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCHH
RGRLDRALAILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATKS
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
GLMVGLGETTDEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVREYVHPDTFKMYEEEAY
CEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHH
KMGFTHAAVGAMVRSSYHADLQAHGAGVV
HHCCHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
TDLTATPAPAEPAASAYDPTAKQKAQAKTARIPIKIVPIEKLKKPEWIRVKAATGSSRF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHCCCCEEEEEECCCCHHH
NEIKTILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD
HHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCC
ADEPKNLARTIAALKLKYVVITSVDRDDLRDGGAGHFVECIREVREQSPATRIEILTPDF
CCCHHHHHHHHHHHHHEEEEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCHH
RGRLDRALAILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDVATKS
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
GLMVGLGETTDEILQVMRDLRAHDVDMLTIGQYLQPSEHHLPVREYVHPDTFKMYEEEAY
CEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHH
KMGFTHAAVGAMVRSSYHADLQAHGAGVV
HHCCHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA