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The map label for this gene is folA [H]

Identifier: 126453089

GI number: 126453089

Start: 2849258

End: 2849761

Strand: Direct

Name: folA [H]

Synonym: BURPS1106A_2902

Alternate gene names: 126453089

Gene position: 2849258-2849761 (Clockwise)

Preceding gene: 126454405

Following gene: 126452938

Centisome position: 71.44

GC content: 69.84

Gene sequence:

>504_bases
ATGACGACGCTGACCTTGATCGTCGCGCGCGCCCGCAACGGCGTGATCGGCCGCGACAACCGACTTCCGTGGAAACTCCC
CGAGGACCTCGCGTTCTTCAAGCGCACGACGATGGGCGCGCCGATCGTGATGGGCCGCAAGACGCACGAATCGATCGGCC
GGCCGCTGCCGGGGCGCCGCAACATCGTCGTCACGCGCGACGCCGCGCGCCGCTTCGACGGCTGCGACACGGTCACCTCG
CTCGACGACGCGCTCGCGCTCGCCGCGCGCGACGGCGCGGCCGAAGCGTTCCTGATCGGCGGCGCGCAACTGTACGAGGA
AGGCCTGCGCCACGCGGACAAGCTGATCGTCACCGAAATCGATCAGGATTTCGAAGGTGACGCGTCGTTCCCCGCGCCCG
ATCCCGCGCAATGGGAAGCCGTGTCGCGCGACGCGCACCGCGCGGCGCAGCCCAACGATTTCACGTACGCGTTCGTCGTC
TATCGCCGCAGGCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
CCGCGCGCGACGCGCGGCAGCGAAACCGGCGCGGCGCGCCGTCGCATGCCGCGCGATCCGGTAGAATCGTGCCCTTCTTC
CGGCCTCTTTTCTTTCCGTC

Downstream 100 bases:

>100_bases
CGGGCGCGAAGCGCCGCGCGGCCGCCCGCGCGCGACGACGATGCAAAAAAGGCGCCGCTTGCCGCGGCGCCTCAGACTGC
TGACGAACCCCACGTTTTTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTTLTLIVARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDAARRFDGCDTVTS
LDDALALAARDGAAEAFLIGGAQLYEEGLRHADKLIVTEIDQDFEGDASFPAPDPAQWEAVSRDAHRAAQPNDFTYAFVV
YRRRRAG

Sequences:

>Translated_167_residues
MTTLTLIVARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDAARRFDGCDTVTS
LDDALALAARDGAAEAFLIGGAQLYEEGLRHADKLIVTEIDQDFEGDASFPAPDPAQWEAVSRDAHRAAQPNDFTYAFVV
YRRRRAG
>Mature_166_residues
TTLTLIVARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDAARRFDGCDTVTSL
DDALALAARDGAAEAFLIGGAQLYEEGLRHADKLIVTEIDQDFEGDASFPAPDPAQWEAVSRDAHRAAQPNDFTYAFVVY
RRRRAG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=182, Percent_Identity=32.4175824175824, Blast_Score=83, Evalue=9e-17,
Organism=Homo sapiens, GI28827793, Length=156, Percent_Identity=34.6153846153846, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1786233, Length=161, Percent_Identity=44.7204968944099, Blast_Score=139, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI116008026, Length=150, Percent_Identity=34, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24647458, Length=150, Percent_Identity=34, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18480; Mature: 18349

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTLIVARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
CCCEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCC
NIVVTRDAARRFDGCDTVTSLDDALALAARDGAAEAFLIGGAQLYEEGLRHADKLIVTEI
CEEEECHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHEEEEEC
DQDFEGDASFPAPDPAQWEAVSRDAHRAAQPNDFTYAFVVYRRRRAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCC
>Mature Secondary Structure 
TTLTLIVARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
CCEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCC
NIVVTRDAARRFDGCDTVTSLDDALALAARDGAAEAFLIGGAQLYEEGLRHADKLIVTEI
CEEEECHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHEEEEEC
DQDFEGDASFPAPDPAQWEAVSRDAHRAAQPNDFTYAFVVYRRRRAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]