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The map label for this gene is 126453061

Identifier: 126453061

GI number: 126453061

Start: 137082

End: 137957

Strand: Direct

Name: 126453061

Synonym: BURPS1106A_0142

Alternate gene names: NA

Gene position: 137082-137957 (Clockwise)

Preceding gene: 126452138

Following gene: 126451629

Centisome position: 3.44

GC content: 67.47

Gene sequence:

>876_bases
ATGAGAAAGACGACCGTTGCCGTTACGCAGATGGCTTGCGACTGGAACGTCGACGCGAACCTGTCGCGCGCCGAGCGACT
CGTGCGCGACGCGGCCGCGCGCGGCGCGCAGATCGTGCTGCTGCAGGAATTGTTCGAGACGCCGTATTTCTGCATCGACC
AGCACCCCGCGCATCTCGCGCTCGCGCAGCCGTATGAAGGCCATCCGTGGCTTATGCGCTTCGCGTCGCTCGCGCGCGAG
CTCGGCGTCGTGCTGCCGGTGAGCTTCTTCGAGCGCGCCGGCCAGACGCAGTTCAACTCGGTCGCGATCTTCGACGCGGA
CGGCCGCGCGCTCGGCATCTATCGAAAGACGCACATTCCGGACGGCCCGGGCTACACGGAGAAATACTATTTCACGCCGG
GCGACACGGGCTTTCGCGTATGGGATACCGCATACGGGCGGATCGGCGTCGGGATCTGCTGGGATCAGTGGTTTCCGGAG
TGCGCGCGGGCAATGGCGCTCGCGGGCGCGGAGCTGTTGCTGTACCCGAGCGCGATCGGCAGCGAGCCGCACGATGCGTC
GATCGATTCGCGCGCGCATTGGCGCAACGCGCAGCGCGGGCATGCGGCCGCGAACCTGATGCCGGTTGCCGCGAGTAATC
GGGTCGGCGTCGAGCGCGGCGCATCGGGCGAGATCGTGTTTTACGGCAGCTCGTTCATCGCGGGCGCGGACGGCGAGATG
ATCGTCGAATGCGATCGGCACGACGAGGCGATCGCGACGGCCGAATTCGATCTCGATGCGCTCGCGTATCGGCGGCGCGG
CTGGGGCGTGTTCCGGGATCGGCGGCCGGAGTGTTATCGGGCGTTGAGCGAGGGCGCGGCGGATCGCGTGCGATGA

Upstream 100 bases:

>100_bases
CAGTATCCGCTCGGCGAAGGCGAGCGCGCCGCGGTGAAGGCCGCCCGCGCCGCGCTGGCGCCGTATTGGCCCACGCTGCA
TCGATCCTGAGGCGCGCACA

Downstream 100 bases:

>100_bases
CGCGGCGAGCGGGCGGCGGGAGCGCGCTCGCGCGTTGCCGATTGCGTCGGCGAAAAAAGCCGCGCATGTCCGCGCGGCGA
ATCCGTCTGCGCTCGAGGGC

Product: carbon-nitrogen family hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRKTTVAVTQMACDWNVDANLSRAERLVRDAAARGAQIVLLQELFETPYFCIDQHPAHLALAQPYEGHPWLMRFASLARE
LGVVLPVSFFERAGQTQFNSVAIFDADGRALGIYRKTHIPDGPGYTEKYYFTPGDTGFRVWDTAYGRIGVGICWDQWFPE
CARAMALAGAELLLYPSAIGSEPHDASIDSRAHWRNAQRGHAAANLMPVAASNRVGVERGASGEIVFYGSSFIAGADGEM
IVECDRHDEAIATAEFDLDALAYRRRGWGVFRDRRPECYRALSEGAADRVR

Sequences:

>Translated_291_residues
MRKTTVAVTQMACDWNVDANLSRAERLVRDAAARGAQIVLLQELFETPYFCIDQHPAHLALAQPYEGHPWLMRFASLARE
LGVVLPVSFFERAGQTQFNSVAIFDADGRALGIYRKTHIPDGPGYTEKYYFTPGDTGFRVWDTAYGRIGVGICWDQWFPE
CARAMALAGAELLLYPSAIGSEPHDASIDSRAHWRNAQRGHAAANLMPVAASNRVGVERGASGEIVFYGSSFIAGADGEM
IVECDRHDEAIATAEFDLDALAYRRRGWGVFRDRRPECYRALSEGAADRVR
>Mature_291_residues
MRKTTVAVTQMACDWNVDANLSRAERLVRDAAARGAQIVLLQELFETPYFCIDQHPAHLALAQPYEGHPWLMRFASLARE
LGVVLPVSFFERAGQTQFNSVAIFDADGRALGIYRKTHIPDGPGYTEKYYFTPGDTGFRVWDTAYGRIGVGICWDQWFPE
CARAMALAGAELLLYPSAIGSEPHDASIDSRAHWRNAQRGHAAANLMPVAASNRVGVERGASGEIVFYGSSFIAGADGEM
IVECDRHDEAIATAEFDLDALAYRRRGWGVFRDRRPECYRALSEGAADRVR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=285, Percent_Identity=31.9298245614035, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=33.9041095890411, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=33.9041095890411, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=33.9041095890411, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI297632346, Length=203, Percent_Identity=35.9605911330049, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI7706509, Length=287, Percent_Identity=28.9198606271777, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533173, Length=281, Percent_Identity=29.8932384341637, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556280, Length=284, Percent_Identity=26.056338028169, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=289, Percent_Identity=27.3356401384083, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322335, Length=230, Percent_Identity=26.9565217391304, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358471, Length=298, Percent_Identity=29.1946308724832, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17933642, Length=280, Percent_Identity=27.8571428571429, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355835, Length=271, Percent_Identity=24.3542435424354, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32295; Mature: 32295

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTTVAVTQMACDWNVDANLSRAERLVRDAAARGAQIVLLQELFETPYFCIDQHPAHLA
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHCCCCEEECCCCCEEE
LAQPYEGHPWLMRFASLARELGVVLPVSFFERAGQTQFNSVAIFDADGRALGIYRKTHIP
ECCCCCCCHHHHHHHHHHHHCCEECCHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCC
DGPGYTEKYYFTPGDTGFRVWDTAYGRIGVGICWDQWFPECARAMALAGAELLLYPSAIG
CCCCCCCEEEECCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECHHHC
SEPHDASIDSRAHWRNAQRGHAAANLMPVAASNRVGVERGASGEIVFYGSSFIAGADGEM
CCCCCCCCCCHHHHCCCCCCCCHHCEEEECCCCCCCCCCCCCCCEEEECCCEEECCCCCE
IVECDRHDEAIATAEFDLDALAYRRRGWGVFRDRRPECYRALSEGAADRVR
EEEECCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MRKTTVAVTQMACDWNVDANLSRAERLVRDAAARGAQIVLLQELFETPYFCIDQHPAHLA
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHCCCCEEECCCCCEEE
LAQPYEGHPWLMRFASLARELGVVLPVSFFERAGQTQFNSVAIFDADGRALGIYRKTHIP
ECCCCCCCHHHHHHHHHHHHCCEECCHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCC
DGPGYTEKYYFTPGDTGFRVWDTAYGRIGVGICWDQWFPECARAMALAGAELLLYPSAIG
CCCCCCCEEEECCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECHHHC
SEPHDASIDSRAHWRNAQRGHAAANLMPVAASNRVGVERGASGEIVFYGSSFIAGADGEM
CCCCCCCCCCHHHHCCCCCCCCHHCEEEECCCCCCCCCCCCCCCEEEECCCEEECCCCCE
IVECDRHDEAIATAEFDLDALAYRRRGWGVFRDRRPECYRALSEGAADRVR
EEEECCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]