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The map label for this gene is pcnB [H]

Identifier: 126452950

GI number: 126452950

Start: 3221598

End: 3223145

Strand: Direct

Name: pcnB [H]

Synonym: BURPS1106A_3304

Alternate gene names: 126452950

Gene position: 3221598-3223145 (Clockwise)

Preceding gene: 126452832

Following gene: 126454085

Centisome position: 80.77

GC content: 70.03

Gene sequence:

>1548_bases
GTGATCAAAAAACTCATCCGCAAGCTGCTCGGGCAGGACGCCCGTACGGCGCAAGACGCGCCGCAGGACCCGGCCGAGCC
GCGCTCGGCCGCGCCCGCCGCCCCGGCGGGCCGCGCGAAACCCGCGCGCGGCGCCGCGAAGAAACCCCGCAACGGCGCGC
CGACCATCGTGCACGCCGACGTGCACGGGATCGACCCCGCGCTCATCTCGAAAAACGCGATCCGCGTGACCGACACGCTG
CAGCAAGCCGGCTTTCGCGCATTCATCGTCGGCGGCGCGGTGCGCGACCTGCTGCTCGGCATCGCGCCGAAGGATTTCGA
CGTCGCGACCGACGCGACGCCAACCGAAGTCCAGCGCCTGTTCCGCCGCGCGCGGCTGATCGGCCGGCGCTTCCAGATCG
TCCACGTGCAGTTCGGCCAGGAGCTGATCGAGGTGTCGACGTTCCGCGCGCTCGTCGACGCGCCGCAGGAGCCGCCCGCC
GACGCCGCGCCCGCGAAGCGCCTGAAGCGCGACGAGCTCGACCGCCGCACGCATGCGGTGGACGCAAGCGGGCGCGTGCT
GCGCGACAACGTGTGGGGCGAGCAGCACGAGGACGCGGCGCGCCGCGATTTCACGATCAACGCGATGTACTACGATCCCG
CATCGCAGACCGTGCTCGATTACCACGACGGGATGGCCGACATGCGCGCGCGCCTGCTGCGCATGATCGGCGATCCGGCG
ACGCGCTACCGCGAAGATCCGGTGCGGATGCTGCGCGTGGTGCGCTTCGCGGCGAAGCTCGGCTTCGACATCGAGGAGAA
GACACGCGAGCCGATCAAGGAGCTCGCCGATCTGATCAACAACGTGCCGGCCGCGCGCCTGTTCGACGAGATGCTCAAGC
TCCTGCTGTCGGGCCACGCGCTCGCGTGCCTGAAGCAGTTGCGCAAGCAGGGGCTGCACCACGGGTTGCTGCCGCTTCTC
GACGTCGTGCTCGAGCAGCCGCAGGGCGAGAAGTTCGTCACGCTCGCGCTGAACAACACCGACGCGCGCGTGCGCGCGGG
CAAGCCGGTGTCGCCCGGCTTCCTGTTCGCGACGCTGCTGTGGCACGACATGCGCCAGCGCTTCGAGCAGTATTCGGCCG
ACGGCGAGTTCCCGGTGCCCGCGCTGCATCGCGCGATGGACGACGTGATCGACATGCAGACCGAGAAGCTCGCGATCCAC
AAGCGCTATTCGGCCGACATGCGCGAGATCTGGGGGTTGCAGTTGCGCCTCGAGAAACGCAGCGGCCGCAGCGCGCTGCG
GCTGCTGGAACACCAAAGATTCAGAGCGGGGTATGATTTCCTCCTGTTACGCTGCGAATCCGGCGAGCTCGATCCGGCCG
TCGGACAGTGGTGGACGGATTTCATCGACGGCGACGCCGCGACGCGCGAGGCGCTCCTCACGCAGGGCGGCAGTCGGGAC
AAGTCGCCCCGCAAGCGGCGCCGCCGCGGCGGACGCAGCCGCCGGACGGCCGACAGCGCCGAGGGCGCGGAAGGCGCCGC
GCGCGGCGAGTCCGACGCGAACGACTGA

Upstream 100 bases:

>100_bases
TCGAAAAAGTCACCGACCCGATCGCGACCAATCCCGACGACACGCTGCGCGCATACGCACGCGAGCACGGCTGGCGCATC
CTCGACCTCTTCCAACCGTC

Downstream 100 bases:

>100_bases
CGCTTGGCTCCGTCGAACGACGTAGGAAGGAAAGCCATGACGGTTGCTTATCTCGGGTTGGGGGCCAATCTCGGCGACGC
GCGCCAGGCCTTGAAGGACG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MIKKLIRKLLGQDARTAQDAPQDPAEPRSAAPAAPAGRAKPARGAAKKPRNGAPTIVHADVHGIDPALISKNAIRVTDTL
QQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPQEPPA
DAAPAKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPA
TRYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHALACLKQLRKQGLHHGLLPLL
DVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYSADGEFPVPALHRAMDDVIDMQTEKLAIH
KRYSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIDGDAATREALLTQGGSRD
KSPRKRRRRGGRSRRTADSAEGAEGAARGESDAND

Sequences:

>Translated_515_residues
MIKKLIRKLLGQDARTAQDAPQDPAEPRSAAPAAPAGRAKPARGAAKKPRNGAPTIVHADVHGIDPALISKNAIRVTDTL
QQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPQEPPA
DAAPAKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPA
TRYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHALACLKQLRKQGLHHGLLPLL
DVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYSADGEFPVPALHRAMDDVIDMQTEKLAIH
KRYSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIDGDAATREALLTQGGSRD
KSPRKRRRRGGRSRRTADSAEGAEGAARGESDAND
>Mature_515_residues
MIKKLIRKLLGQDARTAQDAPQDPAEPRSAAPAAPAGRAKPARGAAKKPRNGAPTIVHADVHGIDPALISKNAIRVTDTL
QQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPQEPPA
DAAPAKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPA
TRYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHALACLKQLRKQGLHHGLLPLL
DVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYSADGEFPVPALHRAMDDVIDMQTEKLAIH
KRYSADMREIWGLQLRLEKRSGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIDGDAATREALLTQGGSRD
KSPRKRRRRGGRSRRTADSAEGAEGAARGESDAND

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=242, Percent_Identity=28.5123966942149, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI87081691, Length=414, Percent_Identity=44.4444444444444, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1789436, Length=255, Percent_Identity=30.1960784313725, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=298, Percent_Identity=26.8456375838926, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57595; Mature: 57595

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLIRKLLGQDARTAQDAPQDPAEPRSAAPAAPAGRAKPARGAAKKPRNGAPTIVHAD
CHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
VHGIDPALISKNAIRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRL
CCCCCHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPQEPPADAAPAKRLKRDELDRRTHAV
HHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHC
DASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPA
CCCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCHH
TRYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHA
HHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
LACLKQLRKQGLHHGLLPLLDVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHH
WHDMRQRFEQYSADGEFPVPALHRAMDDVIDMQTEKLAIHKRYSADMREIWGLQLRLEKR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECC
SGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIDGDAATREALLTQGGSRD
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCC
KSPRKRRRRGGRSRRTADSAEGAEGAARGESDAND
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKKLIRKLLGQDARTAQDAPQDPAEPRSAAPAAPAGRAKPARGAAKKPRNGAPTIVHAD
CHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
VHGIDPALISKNAIRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRL
CCCCCHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPQEPPADAAPAKRLKRDELDRRTHAV
HHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHC
DASGRVLRDNVWGEQHEDAARRDFTINAMYYDPASQTVLDYHDGMADMRARLLRMIGDPA
CCCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCHH
TRYREDPVRMLRVVRFAAKLGFDIEEKTREPIKELADLINNVPAARLFDEMLKLLLSGHA
HHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
LACLKQLRKQGLHHGLLPLLDVVLEQPQGEKFVTLALNNTDARVRAGKPVSPGFLFATLL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHH
WHDMRQRFEQYSADGEFPVPALHRAMDDVIDMQTEKLAIHKRYSADMREIWGLQLRLEKR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECC
SGRSALRLLEHQRFRAGYDFLLLRCESGELDPAVGQWWTDFIDGDAATREALLTQGGSRD
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCC
KSPRKRRRRGGRSRRTADSAEGAEGAARGESDAND
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]