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The map label for this gene is rpoS [H]

Identifier: 126452489

GI number: 126452489

Start: 2225350

End: 2226429

Strand: Reverse

Name: rpoS [H]

Synonym: BURPS1106A_2236

Alternate gene names: 126452489

Gene position: 2226429-2225350 (Counterclockwise)

Preceding gene: 126454413

Following gene: 126453166

Centisome position: 55.82

GC content: 64.54

Gene sequence:

>1080_bases
ATGCCGAAATCGAAGCGCCACGATCCGCAAGCCGAGTCTGAGAAGATCAGTCGAGCCAAGCAAGCATCGGTAGAGCGAAC
TGGTGCTTCAGCGGACGAGGACGAAGACGCCGCCGACAATGAACGCGACTACGAGTCGCGCGATGCGGATCCGGACGAAT
CGGGCGAGGGGCGCGGCGACGCGCAGCCCGATCTGGACGACTTCCGGGCGCTTCTGCAAGCCGAGCTCACCGCCGATACG
ATCCAGCACTACCTGAACCGCATCAGCGTGAAGCCGCTGTTGACGGTCGAGGAAGAGCAGCGCTACTCGCGGCTCGCGAA
GGCGGGCGAATTCGAGGCGCGGCAGGTGATGATCGAGCGCAACCTGCGGCTTGTCGTCAGCATCGCGAAAGGCTATCTGA
ATCGCGGCGTGCCGCTCCTCGATCTGATCGAAGAAGGCAATCTCGGGCTCATGCACGCGATCGAGAAATTCGATCCGACG
CGTGGCTTTCGCTTCTCGACGTACGCGACCTGGTGGATCCGCCAGAGCATCGAGCGCGCGATCATGAATCAGGCGCGGAC
GGTACGGCTGCCGGTGCACGTGATCCGCGAACTGAACCAGGTGCTGCGCGCGAAGCGCCATTTGGAGAAGAACTCGATGT
CCACGGGCGAGGCCGCCGAGCGCCGCGAGGCGAGCATCGACGACATCGCGTATCTGACCGGCAAGACGGCCGAGGAGGTC
ACGGACATCCTCGCGCTGAACGAGCATACGGCGTCGCTCGACGCGCCGCTCGATCTCGACCCGGCGAGCAGCCTGCTCGA
TCTGCTGCCCGACGATCAGAGCCAGTCGCCGGACGCGGAGGTTCAGCACCGCGAGCTGGAGACGCTCACGCGCGCCTGGT
TGTCGCGCCTGTCCGACAAGCACCGCCATGTGATCGAGCGGCGCTTCGGCCTGAACCATATCGAACCCGCGACGCTCGAG
GAGCTTGCCGACGAGATGGGGCTGACCCGCGAACGGGTTCGCCAGATCCAGCAGGAAGCGCTCGTGCGGCTCAAGCGGTT
TTTCGCATCCAACGGCGTGCGCAAGGACGCCGTTCTGTAA

Upstream 100 bases:

>100_bases
ATAGTGATTCCGACCGCGTGATGCTGCATTTCGAGGTTCGCCGGCAGGGTAAACCTGTCGATCCGCTGAAGTATTTGCCG
CCTCAATAAACGAGACGACC

Downstream 100 bases:

>100_bases
TTCATCAATGACACCGATTCTGGTTTTTGACATCGAGACGATTCCCGATGTCGACGGCATCCGCCGTCTCGAAGATCTGC
CCGCCGACCTCGCCGACGCC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPKSKRHDPQAESEKISRAKQASVERTGASADEDEDAADNERDYESRDADPDESGEGRGDAQPDLDDFRALLQAELTADT
IQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPT
RGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTAEEV
TDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPATLE
ELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Sequences:

>Translated_359_residues
MPKSKRHDPQAESEKISRAKQASVERTGASADEDEDAADNERDYESRDADPDESGEGRGDAQPDLDDFRALLQAELTADT
IQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPT
RGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTAEEV
TDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPATLE
ELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
>Mature_358_residues
PKSKRHDPQAESEKISRAKQASVERTGASADEDEDAADNERDYESRDADPDESGEGRGDAQPDLDDFRALLQAELTADTI
QHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTR
GFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTAEEVT
DILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPATLEE
LADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=50.1779359430605, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=37.8787878787879, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789871, Length=282, Percent_Identity=28.0141843971631, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 40832; Mature: 40701

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSKRHDPQAESEKISRAKQASVERTGASADEDEDAADNERDYESRDADPDESGEGRGD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQPDLDDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHHHHHH
NLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQSIERA
CHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHH
IMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTAEEV
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCHHHH
TDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDK
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
PKSKRHDPQAESEKISRAKQASVERTGASADEDEDAADNERDYESRDADPDESGEGRGD
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQPDLDDFRALLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHHHHHH
NLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQSIERA
CHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHH
IMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTAEEV
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCHHHH
TDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDK
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]