Click here to switch to the map view.

The map label for this gene is mdlC [H]

Identifier: 126452093

GI number: 126452093

Start: 874834

End: 876453

Strand: Direct

Name: mdlC [H]

Synonym: BURPS1106A_0890

Alternate gene names: 126452093

Gene position: 874834-876453 (Clockwise)

Preceding gene: 126453044

Following gene: 126452957

Centisome position: 21.93

GC content: 73.33

Gene sequence:

>1620_bases
ATGACGCCCCCCGATCTTCGCGCCGACGCGCCCGCGCGCGCGCCGTACACGGTGCGCGACGCCGTGATCGATCTGCTGCG
CCGGTTCGGCATCCGCAAAGTATTCGGCAATCCGGGCTCGACCGAGCTGCCGATGTTCCGCAATTTCCCCGACGATTTCG
AATACGTGCTCGGCCTGCACGAGGCGGTGGCCGTCGGGATGGCGGACGGCTACGCGCAGGCGAGCGGCAACGCCGCCGTC
GTGAATCTGCACTCGGCGGCGGGCGTCGGCAACGCGATGGGCGTGCTGTTCACCGCGTACCGGAACAAGACGCCGCTCGT
CGTCACGGCCGGCCAGCAGGCGCGCTCGATCCTGCCGTTCGAGCCGTTTCTCGCGTCGGTGCAGGCGGCCGAATTGCCGA
AGCCGTACGTGAAATGGAGCATCGAGCCCGCGCGCGCCGAGGACGTGCCCGCCGCGATCGCGCGCGCGTATCACGTCGCG
ATGCAGGAGCCGCGCGGGCCCGTGTTCGTGTCGATTCCGGCCGACGACTGGGATCGCCCCGCCGAGCCCATGCCCGCGCG
CACGGTGAGCAGCGAAGTGCGGCCCGCGCCCGAGGCGCTCGCGCGGCTCGGCGACGCGCTCGACGCGAGCGAGCGCCCGG
CGTTCGTGGTCGGCGCGGCGGTGGCGCGCGCGGGCGCGTGGGACGACGCGGTGCGGCTCGCCGAGCGGCACCGCGCGCGC
GTGTACGCCGCGCCGATGTCGGGCCGTTGCAGCTTTCCCGAGGACCATCCGCTCTTCGCGGGCTTTCTGCCGCCGATCCG
CGAGCGCATCGTCGAACAGCTCGCCGGCCACGATTTCATCCTCGCGTTCGGCGCGCCCGTCTTCACGTATCACATCGAGG
GCTTCGGCCCGCACGTGCCCGCGGGCGCGACGCTGCATCAGCTCGTCGACGATCCGTCGGTGGCCGCGTGGACGCCCGTG
GGCGACGCGGTCGTCGGCAATCTGCGGCTCGCCGCGCGCGATCTGCTCGCGCGCGCCGCGCCGCGCGAGCGGCCGATGCC
CGCGCCGCGCGCGCCGCGCGGGCGCGTCGGCCCGGGCGCGCCCGGCGAGCGGATGCCGGTGGCGTTCGCGTTGCAGACGC
TCGCCGACGTGCGCGGCTCGCGCGACGTCGTCGTCGAGGAAGCGCCGAGCGCGCGGCCGGCCATGCACGAGCACCTGCCG
TTCTCGAGCGCCGACACGTTCTACACGATGGACAGCGGCGGCCTCGGTTACGGGATGCCGGCCGCTCTCGGCATCGCGCT
CGGCTCGCCGGGGCGGCGCGTGATCGCGCTGATCGGCGACGGCTCGAGCCTCTATTCGATTCAGGCGCTCTGGAGCGCCG
TGCATCTGAAGCTGCCCGTCACGTTCGTGATCCTGAACAACGCGCGCTACGCGGCGCTGCAGAATTTCGCGCCCGTGTTC
GGCTTTCGCGACGGCGAGCCGGTGCAGGGCACCGCGCTGCCCGGTCTCGATTTCGTGTCGCTCGCGCGCGGGTTCGGTTG
CGACGGCGTGCGCGTGAGCGACGCGGCGCGCTTGTGCGACGCGCTCGAGCGGGCGCTCGCGTCGCCCGTGCCGGTCGTCG
TCGACGTCGACGTCGCGTGA

Upstream 100 bases:

>100_bases
GCTGCCGTGTAATGGCTTCTTGCAAAAAATACAGTATCGAGGAATGTGCGCGCCGCCGATACTGTGGAAACCGATGATGA
CGATGAGGAGACACGCCCGC

Downstream 100 bases:

>100_bases
CGCGCGAAGCCGGGAGGACGCAGAGGCCCGAAGGGCCGAAATGCCCGAAGCCCGAAGCCCGAAGCCCGAAGCCCGTGCCA
CGGCATTCACGGGTATCACG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTPPDLRADAPARAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRNFPDDFEYVLGLHEAVAVGMADGYAQASGNAAV
VNLHSAAGVGNAMGVLFTAYRNKTPLVVTAGQQARSILPFEPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVA
MQEPRGPVFVSIPADDWDRPAEPMPARTVSSEVRPAPEALARLGDALDASERPAFVVGAAVARAGAWDDAVRLAERHRAR
VYAAPMSGRCSFPEDHPLFAGFLPPIRERIVEQLAGHDFILAFGAPVFTYHIEGFGPHVPAGATLHQLVDDPSVAAWTPV
GDAVVGNLRLAARDLLARAAPRERPMPAPRAPRGRVGPGAPGERMPVAFALQTLADVRGSRDVVVEEAPSARPAMHEHLP
FSSADTFYTMDSGGLGYGMPAALGIALGSPGRRVIALIGDGSSLYSIQALWSAVHLKLPVTFVILNNARYAALQNFAPVF
GFRDGEPVQGTALPGLDFVSLARGFGCDGVRVSDAARLCDALERALASPVPVVVDVDVA

Sequences:

>Translated_539_residues
MTPPDLRADAPARAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRNFPDDFEYVLGLHEAVAVGMADGYAQASGNAAV
VNLHSAAGVGNAMGVLFTAYRNKTPLVVTAGQQARSILPFEPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVA
MQEPRGPVFVSIPADDWDRPAEPMPARTVSSEVRPAPEALARLGDALDASERPAFVVGAAVARAGAWDDAVRLAERHRAR
VYAAPMSGRCSFPEDHPLFAGFLPPIRERIVEQLAGHDFILAFGAPVFTYHIEGFGPHVPAGATLHQLVDDPSVAAWTPV
GDAVVGNLRLAARDLLARAAPRERPMPAPRAPRGRVGPGAPGERMPVAFALQTLADVRGSRDVVVEEAPSARPAMHEHLP
FSSADTFYTMDSGGLGYGMPAALGIALGSPGRRVIALIGDGSSLYSIQALWSAVHLKLPVTFVILNNARYAALQNFAPVF
GFRDGEPVQGTALPGLDFVSLARGFGCDGVRVSDAARLCDALERALASPVPVVVDVDVA
>Mature_538_residues
TPPDLRADAPARAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRNFPDDFEYVLGLHEAVAVGMADGYAQASGNAAVV
NLHSAAGVGNAMGVLFTAYRNKTPLVVTAGQQARSILPFEPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVAM
QEPRGPVFVSIPADDWDRPAEPMPARTVSSEVRPAPEALARLGDALDASERPAFVVGAAVARAGAWDDAVRLAERHRARV
YAAPMSGRCSFPEDHPLFAGFLPPIRERIVEQLAGHDFILAFGAPVFTYHIEGFGPHVPAGATLHQLVDDPSVAAWTPVG
DAVVGNLRLAARDLLARAAPRERPMPAPRAPRGRVGPGAPGERMPVAFALQTLADVRGSRDVVVEEAPSARPAMHEHLPF
SSADTFYTMDSGGLGYGMPAALGIALGSPGRRVIALIGDGSSLYSIQALWSAVHLKLPVTFVILNNARYAALQNFAPVFG
FRDGEPVQGTALPGLDFVSLARGFGCDGVRVSDAARLCDALERALASPVPVVVDVDVA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=550, Percent_Identity=26.7272727272727, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=23.8010657193606, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=25.4416961130742, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=25.1760563380282, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=25.1346499102334, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 57247; Mature: 57116

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPDLRADAPARAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRNFPDDFEYVLGLH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHCCCCHHHHHHHHHH
EAVAVGMADGYAQASGNAAVVNLHSAAGVGNAMGVLFTAYRNKTPLVVTAGQQARSILPF
HHHHHHCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCCEEEECCHHHCCCCCC
EPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVAMQEPRGPVFVSIPADDWDRP
HHHHHHHHHHHCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
AEPMPARTVSSEVRPAPEALARLGDALDASERPAFVVGAAVARAGAWDDAVRLAERHRAR
CCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCE
VYAAPMSGRCSFPEDHPLFAGFLPPIRERIVEQLAGHDFILAFGAPVFTYHIEGFGPHVP
EEECCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCC
AGATLHQLVDDPSVAAWTPVGDAVVGNLRLAARDLLARAAPRERPMPAPRAPRGRVGPGA
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PGERMPVAFALQTLADVRGSRDVVVEEAPSARPAMHEHLPFSSADTFYTMDSGGLGYGMP
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCH
AALGIALGSPGRRVIALIGDGSSLYSIQALWSAVHLKLPVTFVILNNARYAALQNFAPVF
HHHHEEECCCCCEEEEEEECCCCHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHCCHHH
GFRDGEPVQGTALPGLDFVSLARGFGCDGVRVSDAARLCDALERALASPVPVVVDVDVA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
TPPDLRADAPARAPYTVRDAVIDLLRRFGIRKVFGNPGSTELPMFRNFPDDFEYVLGLH
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHCCCCHHHHHHHHHH
EAVAVGMADGYAQASGNAAVVNLHSAAGVGNAMGVLFTAYRNKTPLVVTAGQQARSILPF
HHHHHHCCCCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCCEEEECCHHHCCCCCC
EPFLASVQAAELPKPYVKWSIEPARAEDVPAAIARAYHVAMQEPRGPVFVSIPADDWDRP
HHHHHHHHHHHCCCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
AEPMPARTVSSEVRPAPEALARLGDALDASERPAFVVGAAVARAGAWDDAVRLAERHRAR
CCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCE
VYAAPMSGRCSFPEDHPLFAGFLPPIRERIVEQLAGHDFILAFGAPVFTYHIEGFGPHVP
EEECCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCC
AGATLHQLVDDPSVAAWTPVGDAVVGNLRLAARDLLARAAPRERPMPAPRAPRGRVGPGA
CCHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PGERMPVAFALQTLADVRGSRDVVVEEAPSARPAMHEHLPFSSADTFYTMDSGGLGYGMP
CCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCCCCCCEEEECCCCCCCCCH
AALGIALGSPGRRVIALIGDGSSLYSIQALWSAVHLKLPVTFVILNNARYAALQNFAPVF
HHHHEEECCCCCEEEEEEECCCCHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHCCHHH
GFRDGEPVQGTALPGLDFVSLARGFGCDGVRVSDAARLCDALERALASPVPVVVDVDVA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]