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The map label for this gene is ynfL [H]

Identifier: 126451650

GI number: 126451650

Start: 516941

End: 517864

Strand: Direct

Name: ynfL [H]

Synonym: BURPS1106A_0550

Alternate gene names: 126451650

Gene position: 516941-517864 (Clockwise)

Preceding gene: 126453778

Following gene: 126454767

Centisome position: 12.96

GC content: 69.59

Gene sequence:

>924_bases
ATGATCGATCTACGACGCCTGCGCTATTTCATCGTCGTCGCCGAGGAGCTTCATTTCGGCAAGGCGGCCCAGCGTCTGCA
CCTCGCGCAGCCGCCGCTCACGCGGCACATCTCCGCGCTCGAAGGCGAGCTCGGCTTGCGGCTGTTCGATCGCTCGACGC
GCTCGGTGAAGCTCACGGCCGAGGGCGAGCTGTTCCTGCCGCACGCGCGCGACGTGCTCGACGCGGTGCATCGCGCGGAG
ATCGCGTCGCAGCGCGCGGCGCTCGGCAAGGAGGGCAAGGTCGCGCTCGGCTATACGAGCTCGGTGCCGCTTTGCGATGC
ATTCGGCACGCTGATCCGCAATTTCGCGCGCTCGTTTCCGGACATCGAGCTGTCGCTCGTCGAGGCGTCGTCCGCGCAGC
AGGGCCGCAGCATCAAGGAGGGGCTGCTCGACATCGGCATCGGCTGGAAGAATTCCTTCGAGGACTACGAGGGATGCCGG
GTGAAGACGATCGCGGCCGAGCCGCTCGTCGTCGCGGTGTCGCAGGAGCGCGCGCTCGCGAGCGAGGCGTCGCTCGCCGT
CGAGCAACTCGCCGACGAGACGTTCGTGCTGTTTCCGCCGGGCTACGGCTCCACGCTCGACCGCAAGGCGTTCGACCTGT
GCGCGACGGCGGGCTTCACGCCGCGGCGCGGGCCGAGCGCCTCGCAGATGACGACGATGATCGCGCTCGTCGCGGCGGGG
CGCGGCGTCGCGATCGTGCCCGAGGCCGTCTCGACGCTGCGCAAGCCGGGCGTCGCGTACGTGCCGCTCACCGACGAATC
CGCGCTGATCGAGCTCGTGCTGATGTGGCGCGAGGCGGGGCTGTCGCTTGCGGCGCGTTCGTTCGTCGAATGGCACAAGG
CGCATGTCGCGCAGCGCGTCGACGGCATGGTCGGCGTGGGCTGA

Upstream 100 bases:

>100_bases
TCGGAAATGTCGATAACTCGCGACGAGGCGTAGTCTCGCCCATTAAGGCCGATTAATGCCAATACCGAATTGCGACAGAT
TGATACTTGGAACGAAAAAG

Downstream 100 bases:

>100_bases
AGCACGCGCGCTCGTGCGTCGAGACGGACGTCGGGCATGCGGCATCGGACATCGATCGGATGCCGAATGCCGGGCCCGGA
CCTCACTGGTACTGACCGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIDLRRLRYFIVVAEELHFGKAAQRLHLAQPPLTRHISALEGELGLRLFDRSTRSVKLTAEGELFLPHARDVLDAVHRAE
IASQRAALGKEGKVALGYTSSVPLCDAFGTLIRNFARSFPDIELSLVEASSAQQGRSIKEGLLDIGIGWKNSFEDYEGCR
VKTIAAEPLVVAVSQERALASEASLAVEQLADETFVLFPPGYGSTLDRKAFDLCATAGFTPRRGPSASQMTTMIALVAAG
RGVAIVPEAVSTLRKPGVAYVPLTDESALIELVLMWREAGLSLAARSFVEWHKAHVAQRVDGMVGVG

Sequences:

>Translated_307_residues
MIDLRRLRYFIVVAEELHFGKAAQRLHLAQPPLTRHISALEGELGLRLFDRSTRSVKLTAEGELFLPHARDVLDAVHRAE
IASQRAALGKEGKVALGYTSSVPLCDAFGTLIRNFARSFPDIELSLVEASSAQQGRSIKEGLLDIGIGWKNSFEDYEGCR
VKTIAAEPLVVAVSQERALASEASLAVEQLADETFVLFPPGYGSTLDRKAFDLCATAGFTPRRGPSASQMTTMIALVAAG
RGVAIVPEAVSTLRKPGVAYVPLTDESALIELVLMWREAGLSLAARSFVEWHKAHVAQRVDGMVGVG
>Mature_307_residues
MIDLRRLRYFIVVAEELHFGKAAQRLHLAQPPLTRHISALEGELGLRLFDRSTRSVKLTAEGELFLPHARDVLDAVHRAE
IASQRAALGKEGKVALGYTSSVPLCDAFGTLIRNFARSFPDIELSLVEASSAQQGRSIKEGLLDIGIGWKNSFEDYEGCR
VKTIAAEPLVVAVSQERALASEASLAVEQLADETFVLFPPGYGSTLDRKAFDLCATAGFTPRRGPSASQMTTMIALVAAG
RGVAIVPEAVSTLRKPGVAYVPLTDESALIELVLMWREAGLSLAARSFVEWHKAHVAQRVDGMVGVG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=36.8055555555556, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=33.0798479087452, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=28.8659793814433, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790399, Length=305, Percent_Identity=29.1803278688525, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI157672245, Length=252, Percent_Identity=29.3650793650794, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081904, Length=200, Percent_Identity=30, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=253, Percent_Identity=24.901185770751, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=25.2788104089219, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=27.6, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=269, Percent_Identity=23.4200743494424, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=264, Percent_Identity=23.8636363636364, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=255, Percent_Identity=26.2745098039216, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33222; Mature: 33222

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRRLRYFIVVAEELHFGKAAQRLHLAQPPLTRHISALEGELGLRLFDRSTRSVKLTA
CCCHHHEEEEEEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCEEEECCCCCEEEEEE
EGELFLPHARDVLDAVHRAEIASQRAALGKEGKVALGYTSSVPLCDAFGTLIRNFARSFP
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
DIELSLVEASSAQQGRSIKEGLLDIGIGWKNSFEDYEGCRVKTIAAEPLVVAVSQERALA
CCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHH
SEASLAVEQLADETFVLFPPGYGSTLDRKAFDLCATAGFTPRRGPSASQMTTMIALVAAG
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
RGVAIVPEAVSTLRKPGVAYVPLTDESALIELVLMWREAGLSLAARSFVEWHKAHVAQRV
CCEEECHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DGMVGVG
CCCCCCC
>Mature Secondary Structure
MIDLRRLRYFIVVAEELHFGKAAQRLHLAQPPLTRHISALEGELGLRLFDRSTRSVKLTA
CCCHHHEEEEEEEEHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCEEEECCCCCEEEEEE
EGELFLPHARDVLDAVHRAEIASQRAALGKEGKVALGYTSSVPLCDAFGTLIRNFARSFP
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
DIELSLVEASSAQQGRSIKEGLLDIGIGWKNSFEDYEGCRVKTIAAEPLVVAVSQERALA
CCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHH
SEASLAVEQLADETFVLFPPGYGSTLDRKAFDLCATAGFTPRRGPSASQMTTMIALVAAG
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
RGVAIVPEAVSTLRKPGVAYVPLTDESALIELVLMWREAGLSLAARSFVEWHKAHVAQRV
CCEEECHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DGMVGVG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]