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The map label for this gene is mdlB [H]

Identifier: 126447480

GI number: 126447480

Start: 665815

End: 667158

Strand: Reverse

Name: mdlB [H]

Synonym: BMA10247_A0717

Alternate gene names: 126447480

Gene position: 667158-665815 (Counterclockwise)

Preceding gene: 126447960

Following gene: 126447872

Centisome position: 28.36

GC content: 69.05

Gene sequence:

>1344_bases
TTGAGCGTCGGAGTAGCCATGATTATTCGCTTTTATCGAACGATTTCGTTTGAAGATTATCGCTTTTTATCGATGTGGCA
GCTGCATAGAATGGCTGCGTCGCGTCAGGAAAGCCCAACAATGAGCACTGTCACCTCTTCTCCCATCGTCGAAGCGAGCC
GGCCCGCGGCCAGCGCCGCCGCCACGCCGCGCGCGCTGCGGGGTGTCCTGAGCCTGCACGACTTCGAAGCGAGGGCGCGC
CGCGCGCTGCCGCGCCCGATCTTCGGCTACGTCGGCGGCGCGGCCGAAGACAACCGCACCCGCGACGACAACCGAGCCGC
CTTCGACGAATACGGCTTCGTCACGCGCGTGCTGCGCGACGTCTCGCAGCGGCAGCAGGGTGTCGAGCTGTTCGGCCGGC
GCTACGCGTCGCCGTTCGGCATCGCGCCGATGGGCATCAACGCGCTGTCGACGTATCGCGGCGACATCGTGCTCGCGCGC
GCCGCGCAGCACGCGGGGATCGCGTCGATCATGAGCGGCTCGTCGCTGATTCCGCTCGAAGACGTGGCCGCCGCCGCGCC
CGACACGTGGTTCCAGGCCTACCTGCCGGGCGATGCCGGGCGCATCCGCGCGCTCGTCGAGCGCGTCGCGCGCGCCGGCT
ACCGGACGCTCGTCGTCACCGTCGACATTCCGGTGTCGGCGAACCGCGAAAACAACGTGCGCAGCGGCTTCTCCACGCCG
CTGCGCCCGAGCCCGCGCCTGTTCTGGGACGGGCTGACGCGGCCGCGCTGGCTGTTGGGCACGTTCGCGCGCACGTTGCT
CGCGCACGGCATGCCGCATTTCGAGAATTCGTTCGCGACGCGCGGCGCGCCGATTCTTTCCGCCAACGTGCTGCGCGACT
TTTCCGCGCGCGACCATCTGAGCTGGGCGCACGTGCGGCAAATCCGCGAGCAATGGGCGGGCGAGCTCGTGATCAAGGGC
GTGCTCAGCGTCGAGGACGCGCGGATCGCGCGCGAGGCGGGCGCCGACGGCATCATCCTGTCCAACCACGGCGGCCGCCA
GCTGGACGGCGCCGTATCGCCGATGCGGATCCTGCGCGACGTGGTGCAGGCTGTCGGCAACGGGTATCCGGTGATGATCG
ACAGCGGTTTTCGCCGGGGCTCGGACGTGCTGAAGGCCGTGGCGCTCGGCGCGCGGATGGTGTTCGTCGGACGGCCGTTC
AACTATGCGGCCGCGGTGGCGGGCGAGGCCGGCGTCGCGCATGCGATCCGCCTGCTGCGCGAAGAGGTGGACCGGAATCT
GGCGATGCTGGGCGCGAACGGCTGCGACGCGCTGACGCCCGACATGCTGATTCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
GTTGATTCGCTTCGATAAGGACGAGATCGACAGATGAAGCCGCTGCGCGGCGGTCGAGAAATTCGCGCAGGTCGCCGCCC
AATAAAAGGCATCGAGTTGC

Downstream 100 bases:

>100_bases
CGGCGCCGGGCGGTTCGATCGCGAAGGGCCGCCGCGGCGGCGGTCATGCGCGCCCGACGACGCCTCGCCGCCGAGGGTCG
AGGCGTCGTCGGGCGCTTGC

Product: FMN-dependent family dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSVGVAMIIRFYRTISFEDYRFLSMWQLHRMAASRQESPTMSTVTSSPIVEASRPAASAAATPRALRGVLSLHDFEARAR
RALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLAR
AAQHAGIASIMSGSSLIPLEDVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTP
LRPSPRLFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVRQIREQWAGELVIKG
VLSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPF
NYAAAVAGEAGVAHAIRLLREEVDRNLAMLGANGCDALTPDMLIRKR

Sequences:

>Translated_447_residues
MSVGVAMIIRFYRTISFEDYRFLSMWQLHRMAASRQESPTMSTVTSSPIVEASRPAASAAATPRALRGVLSLHDFEARAR
RALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLAR
AAQHAGIASIMSGSSLIPLEDVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTP
LRPSPRLFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVRQIREQWAGELVIKG
VLSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPF
NYAAAVAGEAGVAHAIRLLREEVDRNLAMLGANGCDALTPDMLIRKR
>Mature_446_residues
SVGVAMIIRFYRTISFEDYRFLSMWQLHRMAASRQESPTMSTVTSSPIVEASRPAASAAATPRALRGVLSLHDFEARARR
ALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLARA
AQHAGIASIMSGSSLIPLEDVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTPL
RPSPRLFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVRQIREQWAGELVIKGV
LSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFN
YAAAVAGEAGVAHAIRLLREEVDRNLAMLGANGCDALTPDMLIRKR

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI54234014, Length=366, Percent_Identity=34.4262295081967, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI7705393, Length=366, Percent_Identity=34.4262295081967, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI11068137, Length=380, Percent_Identity=31.5789473684211, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1790033, Length=380, Percent_Identity=36.8421052631579, Blast_Score=220, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI193208036, Length=386, Percent_Identity=32.9015544041451, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI32566217, Length=308, Percent_Identity=29.5454545454545, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323587, Length=390, Percent_Identity=29.2307692307692, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI281363140, Length=380, Percent_Identity=33.6842105263158, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI78707190, Length=380, Percent_Identity=33.6842105263158, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI78707188, Length=380, Percent_Identity=33.6842105263158, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 48850; Mature: 48718

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGVAMIIRFYRTISFEDYRFLSMWQLHRMAASRQESPTMSTVTSSPIVEASRPAASAA
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHH
ATPRALRGVLSLHDFEARARRALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRD
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAGIASIMSGSSLIPLE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEHHHHCCHHHHHCCCCCCCHH
DVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTP
HHHHCCCCHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCC
LRPSPRLFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHL
CCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCHHCCHHCCCCCHHHHHHHHHCCHHCCC
SWAHVRQIREQWAGELVIKGVLSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHH
VVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAVAGEAGVAHAIRLLR
HHHHHCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHHHHHHHHH
EEVDRNLAMLGANGCDALTPDMLIRKR
HHHCCCEEEECCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SVGVAMIIRFYRTISFEDYRFLSMWQLHRMAASRQESPTMSTVTSSPIVEASRPAASAA
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHH
ATPRALRGVLSLHDFEARARRALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRD
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAGIASIMSGSSLIPLE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEHHHHCCHHHHHCCCCCCCHH
DVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTP
HHHHCCCCHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCC
LRPSPRLFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHL
CCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCHHCCHHCCCCCHHHHHHHHHCCHHCCC
SWAHVRQIREQWAGELVIKGVLSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHH
VVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAVAGEAGVAHAIRLLR
HHHHHCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHHHHHHHHH
EEVDRNLAMLGANGCDALTPDMLIRKR
HHHCCCEEEECCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]