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The map label for this gene is xylX [H]

Identifier: 126447088

GI number: 126447088

Start: 199549

End: 200826

Strand: Direct

Name: xylX [H]

Synonym: BMA10247_A0234

Alternate gene names: 126447088

Gene position: 199549-200826 (Clockwise)

Preceding gene: 126446195

Following gene: 126446189

Centisome position: 8.48

GC content: 64.48

Gene sequence:

>1278_bases
ATGGAAATGACCGAATCGCCGGTGGCGCTGATCGATCGCGCCGATTCGTCCGACGTGCGCTTTCCGCGCGACGACGGCTC
GCGCGTGCCGTACAAGGTGTTCAGCTCGCAGGCGGTGTACGAGCGCGAGCAGGAGCGGATCTTCCGCGGGCCGACGTGGA
ATTTCGTCGCGCTCGAAGCGGAAATTCCGAAGCCGGGCGATTTCAAGAGCACGTTCGTCGGCGACACGCCCGTCGTCGTC
ACGCGCGGCGAGGACGGCGCGCTCGCCGCGTGGGTGAACCGCTGCGCGCATCGCGGCGCGCAGGTGTGCCGCAAGGCGCG
CGGCAACGCGAGCTCGCACACGTGCGTGTATCACCAGTGGAGCTTCGACAGCCGGGGCGACCTGCTCGGCGTGCCGTTCC
GGCGCGGCCAGAAGGGCATGGCCGGGATGCCGGCCGATTTCGATCCGAAGAGCCACGGGCTGCGCAAGCTGCGCGTCGAC
AGCTACAAGGGCCTCGTGTTCGCGACGTTCAGCGACGAGGTCGCGCCGCTGCCCGATTACCTCGGCGCGCAGATGCGGCC
GTGGATCGACCGGATCTTCCACAAGCCGATCGAGTATCTCGGCTGCACGCGGCAGTTCTCGAAATCGAACTGGAAGCTGT
ACTTCGAGAACGTGAAGGACCCGTATCACGCGAGCATGCTGCATCTGTTCCATACGACGTTCAATATTTTCCGCGTCGGC
ATGAAGGCGCGCTCGATTCCCGATGCGACGCACGGGCTGCACAGCATCATCACGATGACGAAGACGCGCGACGACGCGGA
CACCGCGAGCGCGTACAAGCAGCAGAACATCCGTTCGTTCGACGAAGGTTTCTCGCTCGAGGACGATTCGATCCTCGGCC
TCGTGTCCGAGTACGACGAGGACACGACGAACCACATCCAGCCGATCTTCCCGCAGCTCGTGATCCAGCAGATCCACAAC
ACGCTCGTCGCACGCCAGTTGCTGCCGAAGGGGCCGAAGAACTTCGAATTGATCTTCCACTTCTTCGGCTATGCGGACGA
CACGCCCGAGCTGCGCGATTTGCGGATCAAGCAGGCGAACCTCGTCGGGCCCGCGGGCTACATCTCGATGGAGGACACCG
AGGCGACCGAGCTCGTGCAGCGCGGCACCGTGCGCGACGCAAACGCGGCGTCGGTGATCGAGATGTCGCGCGGCAATCCG
GACCAGCAGGACACGGCGATCACCGAGAGCCTGATCCGCAGGTTCTGGGTCGGCTACCAGCAGTTGATGGGCTATTGA

Upstream 100 bases:

>100_bases
TTCAAAAATCGGTCTCGTCAGGCGGATTGTCCAGCGCCTCAACCGCAGCAACTTGCATGGGATCGGAGCGGGCGCCCGCT
CCGACCGACAGGAGACAATG

Downstream 100 bases:

>100_bases
AGCGGGAGACGAACATGACCGACATCACCGACGGCCTCACCGGCGACATTCGCACGTGGTTCGAGCTGCACATGCTGCAG
AACCGCTACATCGGGCATCT

Product: aromatic-ring-hydroxylating dioxygenase, alpha subunit

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MEMTESPVALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGDFKSTFVGDTPVVV
TRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGDLLGVPFRRGQKGMAGMPADFDPKSHGLRKLRVD
SYKGLVFATFSDEVAPLPDYLGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVG
MKARSIPDATHGLHSIITMTKTRDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQPIFPQLVIQQIHN
TLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNP
DQQDTAITESLIRRFWVGYQQLMGY

Sequences:

>Translated_425_residues
MEMTESPVALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGDFKSTFVGDTPVVV
TRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGDLLGVPFRRGQKGMAGMPADFDPKSHGLRKLRVD
SYKGLVFATFSDEVAPLPDYLGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVG
MKARSIPDATHGLHSIITMTKTRDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQPIFPQLVIQQIHN
TLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNP
DQQDTAITESLIRRFWVGYQQLMGY
>Mature_425_residues
MEMTESPVALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEAEIPKPGDFKSTFVGDTPVVV
TRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQWSFDSRGDLLGVPFRRGQKGMAGMPADFDPKSHGLRKLRVD
SYKGLVFATFSDEVAPLPDYLGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVG
MKARSIPDATHGLHSIITMTKTRDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDEDTTNHIQPIFPQLVIQQIHN
TLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQANLVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNP
DQQDTAITESLIRRFWVGYQQLMGY

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=412, Percent_Identity=27.9126213592233, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788103, Length=203, Percent_Identity=26.6009852216749, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 48203; Mature: 48203

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMTESPVALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEA
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEEE
EIPKPGDFKSTFVGDTPVVVTRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQW
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
SFDSRGDLLGVPFRRGQKGMAGMPADFDPKSHGLRKLRVDSYKGLVFATFSDEVAPLPDY
CCCCCCCEECCCHHCCCCCCCCCCCCCCCHHCCCEEEEECCCCCEEEEECCCCCCCCHHH
LGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVG
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
MKARSIPDATHGLHSIITMTKTRDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDE
HHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
DTTNHIQPIFPQLVIQQIHNTLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHEEECCCCCCCCHHHHHEEHHHC
LVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAITESLIRRFWVGYQ
CCCCCCEEECCCCHHHHHHHHCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHH
QLMGY
HHHCC
>Mature Secondary Structure
MEMTESPVALIDRADSSDVRFPRDDGSRVPYKVFSSQAVYEREQERIFRGPTWNFVALEA
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEEE
EIPKPGDFKSTFVGDTPVVVTRGEDGALAAWVNRCAHRGAQVCRKARGNASSHTCVYHQW
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
SFDSRGDLLGVPFRRGQKGMAGMPADFDPKSHGLRKLRVDSYKGLVFATFSDEVAPLPDY
CCCCCCCEECCCHHCCCCCCCCCCCCCCCHHCCCEEEEECCCCCEEEEECCCCCCCCHHH
LGAQMRPWIDRIFHKPIEYLGCTRQFSKSNWKLYFENVKDPYHASMLHLFHTTFNIFRVG
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
MKARSIPDATHGLHSIITMTKTRDDADTASAYKQQNIRSFDEGFSLEDDSILGLVSEYDE
HHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
DTTNHIQPIFPQLVIQQIHNTLVARQLLPKGPKNFELIFHFFGYADDTPELRDLRIKQAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHEEECCCCCCCCHHHHHEEHHHC
LVGPAGYISMEDTEATELVQRGTVRDANAASVIEMSRGNPDQQDTAITESLIRRFWVGYQ
CCCCCCEEECCCCHHHHHHHHCCCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHH
QLMGY
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]