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The map label for this gene is nadE

Identifier: 126446643

GI number: 126446643

Start: 1591934

End: 1592788

Strand: Direct

Name: nadE

Synonym: BMA10247_A1653

Alternate gene names: 126446643

Gene position: 1591934-1592788 (Clockwise)

Preceding gene: 126446586

Following gene: 126447086

Centisome position: 67.66

GC content: 70.06

Gene sequence:

>855_bases
ATGTCCCGTCCCGATCAGGCCGCCCGCCGGCGTGCGATCGCCGCCGAACTGCACGTGTCGCCCACGTTCGATGCGCGCGA
CGAAGCCGAGCGCCGCATCGGGTTCGTCGCCGATTATCTGCGCACGGCCGGATTGCGGGCCTGCGTGCTGGGCATCAGCG
GCGGCATCGATTCGTCGACGGCGGGCCGGCTCGCGCAACTGGCCGTCGAGCGGCTGCGCGCATCGGGCTACGACGCGCGC
TTCGTCGCGATGCGGCTGCCGTACGGCGCGCAGCACGACGAAGCGGACGCGCGGCGCGCGCTCGCCTTCGTGCGCGCGGA
CGAGACGCTCACGGTCGACGTGAAGCCGGCCGCCGATGCGATGCTCGCCGCGCTCGCGGCGGGCGGCCTGGCGTATCTCG
ACCACGCACAGCAGGATTTCGTGCTCGGCAACATCAAGGCGCGCGAGCGGATGATCGCGCAATACGCGGTGGCGGGCGCG
CGGAACGGCGTCGTGATCGGCACCGATCATGCGGCGGAATCGGTGATGGGCTTCTTCACGAAGTTCGGCGACGGCGGCGC
GGACGTGCTGCCGCTCGCGGGCCTCACGAAACGCCGCGTGCGCGCACTCGCGCGCATGCTGGGCGCCGACGAGCCGCTTG
TGCTGAAAACGCCGACCGCCGATCTCGAAACGTTGCGTCCGCAACGGCCCGACGAGCACGCGTACGGCATCACGTACGAG
CAGATCGACGATTTTCTCGAAGGCAAGCCGATGGACGACGCGGTCGCCGAGACGGTGCTGCGCTTCTATGACGCGACACA
CCACAAGCGCGCGCTGCCCTATACGATGTTCGACTGGCCGGGCCACCCGGCATGA

Upstream 100 bases:

>100_bases
CGACACCCGACGACCGGATCGACAGCCGAATCCGCCGCCGGCAAGCCCGCGCGAATCCGCCTATGATCGGTTCTTCGATT
CCGCCGCGAAGGAGCACGCG

Downstream 100 bases:

>100_bases
GCGAGGAGGAAGACGAAGCCCGCGGCGGCTCGGCGCCGCCGGTTCGGCGGTCGTTTCCGCCGCCGCGCCGCTGCGGTCCA
CGATCGCGCACGCGCGCCGC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSSTAGRLAQLAVERLRASGYDAR
FVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADAMLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGA
RNGVVIGTDHAAESVMGFFTKFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
QIDDFLEGKPMDDAVAETVLRFYDATHHKRALPYTMFDWPGHPA

Sequences:

>Translated_284_residues
MSRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSSTAGRLAQLAVERLRASGYDAR
FVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADAMLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGA
RNGVVIGTDHAAESVMGFFTKFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
QIDDFLEGKPMDDAVAETVLRFYDATHHKRALPYTMFDWPGHPA
>Mature_283_residues
SRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSSTAGRLAQLAVERLRASGYDARF
VAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADAMLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGAR
NGVVIGTDHAAESVMGFFTKFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYEQ
IDDFLEGKPMDDAVAETVLRFYDATHHKRALPYTMFDWPGHPA

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=272, Percent_Identity=48.1617647058824, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_BURM7 (A3MF00)

Other databases:

- EMBL:   CP000547
- RefSeq:   YP_001078836.1
- ProteinModelPortal:   A3MF00
- SMR:   A3MF00
- GeneID:   4890081
- GenomeReviews:   CP000547_GR
- KEGG:   bmn:BMA10247_A1653
- TIGR:   BMA10247_A1653
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   BMAL320389:BMA10247_A1653-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30863; Mature: 30732

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSST
CCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHH
AGRLAQLAVERLRASGYDARFVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEECCCEEEEECCCHHHH
MLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGARNGVVIGTDHAAESVMGFFT
HHHHHHHCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
KFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
HHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHH
QIDDFLEGKPMDDAVAETVLRFYDATHHKRALPYTMFDWPGHPA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSST
CCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHH
AGRLAQLAVERLRASGYDARFVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEECCCEEEEECCCHHHH
MLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGARNGVVIGTDHAAESVMGFFT
HHHHHHHCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
KFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
HHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHH
QIDDFLEGKPMDDAVAETVLRFYDATHHKRALPYTMFDWPGHPA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA