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The map label for this gene is pitA [H]

Identifier: 126446156

GI number: 126446156

Start: 657272

End: 658858

Strand: Reverse

Name: pitA [H]

Synonym: BMA10247_A0709

Alternate gene names: 126446156

Gene position: 658858-657272 (Counterclockwise)

Preceding gene: 126447872

Following gene: 126446716

Centisome position: 28.0

GC content: 69.44

Gene sequence:

>1587_bases
ATGAATCCCCTTCTGTCCGCCGCGGCGAGCGAAGAGCTCGGTCGCACCCGGCATCTGGGCGTCGCGCTGCTCGTCGCCCT
CCTCGCCGCCGGCGTCGCGTACGTGTTCGTTCACCTGTTCGCGGATCTCGCGCCCGTCGGCCCACGCTCGGCGTTTCCGT
ATCTGCTGCTCGGCGTCGCGCTCGCGATCGCGCTCGGCTTCGAGTTCGTCAACGGCTTCCACGACACCGCGAACGCCGTG
GCGACCGTGATCTACACGCATTCGCTGACGCCGAACGTCGCGGTCATCTGGTCGGGCCTCTGGAATTTTCTCGGCGTGAT
GGCGTCGACGGGCGCGGTCGCGTTCGGCATCCTGCAACTGCTGCCCGTCGAGCTGATCCTGCAAGTCGGCCACGGCGCGG
GCTTCGCGATGGTGTTCGCGCTGCTGATCGCGGCGATCGCGTGGAATCTCGCGACCTGGTACTTCGGCCTGCCTTCGTCG
AGCTCGCACACGCTGATCGGCTCGATCATCGGCGTCGGGCTCGCGAATCAGCTGATGCACGGGCCGTCCGGCACGAGCGG
CGTCGATTGGGCGCAGGCGGCGGGCGTCGGCCAATCGCTGCTGCTCTCGCCGCTCGTCGGCTTCCTGTGCGCGGCCCTGC
TGCTCGCCGCGCTGAAGGCGCTCGTGCGCGTGCCCGCGCTCTACCGCGAGCCGCAGACGAACGAGCCGCCGCCGTTCTGG
GTCCGCTGCCTGCTGATCCTGACCTGCACCGGCGTGTCGTTCGCGCACGGCTCGAACGACGGGCAGAAAGGCATGGGGCT
CATCATGCTGATCCTGATCGGCACCGTGCCGACCGCATACGCGCTCAACAAGGCGGTCACGCCCGGCGAGACGCAAACGT
TCATCGCCGTCGCACACCGCGCGTCCGAAACGCTCGCCCGCTACGCGGGCGCCGCGCAGCCGTCGCCGAACCCGCGCGCC
GATGTCGAGGCTTACGTGCAGTCGCGCCGCCTGAGCGACGCGACGCTGCCCGCGGTTCGCCGGTTGTCGGACGCGCTCGC
GGCCGAAGTCGGCGCGACGGGCTCGATCGCGAAAGTGCCCGAGGGGCAGGCGGACAACGTGCGCAACACGATGTATCTGG
TGTCCGAAGCCCTGCGCCTGATGGAGAAAACCGGCCGGCCGGCGTTCTCGCCGGCGGATCGCCTCGCGCTCGACGACTAC
CGCAGGCAGCTCGACCACGCGACCAAATTCATTCCGACCTGGGTGAAGGTCGCGGTGGCGATCGCGCTCGGCCTCGGCAC
GATGGTCGGCTGGCGGCGCATCGTCGTGACCGTCGGCGAGAAGATCGGCAAGCAGCATCTGACCTATGGGCAAGGCGCGT
CGGCGGAAGTCGTCGCGATGCTCACGATCGGCGCGGCCGATGCGTACGGGCTGCCGGTGTCGACGACGCACGTGCTGTCT
TCCGGCGTCGCGGGCACGATGGCCGCGAACGGCTCCGGGCTGCAATGGAGCACGGTGCGCAGCCTCGTGCTCGCGTGGGT
GTTGACGCTGCCCGCGTCGATCGTGCTCGCGGGCGCGCTCTATTGGGCTTTCCACGCGCTGACCTGA

Upstream 100 bases:

>100_bases
GAGTCGCACACCCGAATCGCCCCCCCCCTTTGCGCGCTGTCATCAAACTGCCTGATCGCGAGGGCTAGGATCGAACGATC
CGTTCCAAACAAGGGCCGTC

Downstream 100 bases:

>100_bases
CGCGCATGGCGGCGTGACGGCCCGCGCGCGAGACATCGCGCGAAACATAATGCGCGGCCGAATTGCAGCGCAAGATCCGG
TGTGCATCGCTTCGCCCGCC

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MNPLLSAAASEELGRTRHLGVALLVALLAAGVAYVFVHLFADLAPVGPRSAFPYLLLGVALAIALGFEFVNGFHDTANAV
ATVIYTHSLTPNVAVIWSGLWNFLGVMASTGAVAFGILQLLPVELILQVGHGAGFAMVFALLIAAIAWNLATWYFGLPSS
SSHTLIGSIIGVGLANQLMHGPSGTSGVDWAQAAGVGQSLLLSPLVGFLCAALLLAALKALVRVPALYREPQTNEPPPFW
VRCLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNKAVTPGETQTFIAVAHRASETLARYAGAAQPSPNPRA
DVEAYVQSRRLSDATLPAVRRLSDALAAEVGATGSIAKVPEGQADNVRNTMYLVSEALRLMEKTGRPAFSPADRLALDDY
RRQLDHATKFIPTWVKVAVAIALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMLTIGAADAYGLPVSTTHVLS
SGVAGTMAANGSGLQWSTVRSLVLAWVLTLPASIVLAGALYWAFHALT

Sequences:

>Translated_528_residues
MNPLLSAAASEELGRTRHLGVALLVALLAAGVAYVFVHLFADLAPVGPRSAFPYLLLGVALAIALGFEFVNGFHDTANAV
ATVIYTHSLTPNVAVIWSGLWNFLGVMASTGAVAFGILQLLPVELILQVGHGAGFAMVFALLIAAIAWNLATWYFGLPSS
SSHTLIGSIIGVGLANQLMHGPSGTSGVDWAQAAGVGQSLLLSPLVGFLCAALLLAALKALVRVPALYREPQTNEPPPFW
VRCLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNKAVTPGETQTFIAVAHRASETLARYAGAAQPSPNPRA
DVEAYVQSRRLSDATLPAVRRLSDALAAEVGATGSIAKVPEGQADNVRNTMYLVSEALRLMEKTGRPAFSPADRLALDDY
RRQLDHATKFIPTWVKVAVAIALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMLTIGAADAYGLPVSTTHVLS
SGVAGTMAANGSGLQWSTVRSLVLAWVLTLPASIVLAGALYWAFHALT
>Mature_528_residues
MNPLLSAAASEELGRTRHLGVALLVALLAAGVAYVFVHLFADLAPVGPRSAFPYLLLGVALAIALGFEFVNGFHDTANAV
ATVIYTHSLTPNVAVIWSGLWNFLGVMASTGAVAFGILQLLPVELILQVGHGAGFAMVFALLIAAIAWNLATWYFGLPSS
SSHTLIGSIIGVGLANQLMHGPSGTSGVDWAQAAGVGQSLLLSPLVGFLCAALLLAALKALVRVPALYREPQTNEPPPFW
VRCLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNKAVTPGETQTFIAVAHRASETLARYAGAAQPSPNPRA
DVEAYVQSRRLSDATLPAVRRLSDALAAEVGATGSIAKVPEGQADNVRNTMYLVSEALRLMEKTGRPAFSPADRLALDDY
RRQLDHATKFIPTWVKVAVAIALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMLTIGAADAYGLPVSTTHVLS
SGVAGTMAANGSGLQWSTVRSLVLAWVLTLPASIVLAGALYWAFHALT

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=473, Percent_Identity=43.7632135306554, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789360, Length=473, Percent_Identity=41.2262156448203, Blast_Score=355, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 55238; Mature: 55238

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLSAAASEELGRTRHLGVALLVALLAAGVAYVFVHLFADLAPVGPRSAFPYLLLGVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAIALGFEFVNGFHDTANAVATVIYTHSLTPNVAVIWSGLWNFLGVMASTGAVAFGILQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVELILQVGHGAGFAMVFALLIAAIAWNLATWYFGLPSSSSHTLIGSIIGVGLANQLMH
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
GPSGTSGVDWAQAAGVGQSLLLSPLVGFLCAALLLAALKALVRVPALYREPQTNEPPPFW
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VRCLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNKAVTPGETQTFIAVAHR
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHH
ASETLARYAGAAQPSPNPRADVEAYVQSRRLSDATLPAVRRLSDALAAEVGATGSIAKVP
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGQADNVRNTMYLVSEALRLMEKTGRPAFSPADRLALDDYRRQLDHATKFIPTWVKVAVA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMLTIGAADAYGLPVSTTHVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
SGVAGTMAANGSGLQWSTVRSLVLAWVLTLPASIVLAGALYWAFHALT
CCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPLLSAAASEELGRTRHLGVALLVALLAAGVAYVFVHLFADLAPVGPRSAFPYLLLGVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAIALGFEFVNGFHDTANAVATVIYTHSLTPNVAVIWSGLWNFLGVMASTGAVAFGILQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVELILQVGHGAGFAMVFALLIAAIAWNLATWYFGLPSSSSHTLIGSIIGVGLANQLMH
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
GPSGTSGVDWAQAAGVGQSLLLSPLVGFLCAALLLAALKALVRVPALYREPQTNEPPPFW
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VRCLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAYALNKAVTPGETQTFIAVAHR
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHH
ASETLARYAGAAQPSPNPRADVEAYVQSRRLSDATLPAVRRLSDALAAEVGATGSIAKVP
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGQADNVRNTMYLVSEALRLMEKTGRPAFSPADRLALDDYRRQLDHATKFIPTWVKVAVA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMLTIGAADAYGLPVSTTHVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
SGVAGTMAANGSGLQWSTVRSLVLAWVLTLPASIVLAGALYWAFHALT
CCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]