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The map label for this gene is potF [H]

Identifier: 126445558

GI number: 126445558

Start: 691859

End: 693088

Strand: Reverse

Name: potF [H]

Synonym: BURPS668_A0726

Alternate gene names: 126445558

Gene position: 693088-691859 (Counterclockwise)

Preceding gene: 126444673

Following gene: 126443131

Centisome position: 22.16

GC content: 64.39

Gene sequence:

>1230_bases
GTGCGATCGATGCGACGGCGCAGCAACTGGGTCTTTCCTAACGGCATTGCCAATCGGGCAGGCGGCGCGCGGGCGCCGCG
CGCCCACCTTCATCATCAGGGGAACAACATGAGTGTGAGCCATCTTCGTCATGCGGTCGCGCGCGCAGCGCTCGTCGCGC
TCGCCGGCGCGTCGGCGCTCGCCCTGCCGGCCGCGCACGCGGCCGGCGCCGAGCTGAACGTGTACAACTGGTCGGACTAC
ATCGCGAAAGACACGATCCCGAACTTCGAGAAGCAATCCGGCATCCACGTCAAGTACGACAACTACGACAGCGACGACAC
GCTGCAGGCGAAGCTGCTCGCGGGCAGCTCCGGCTACGACATCGTCGTGCCGACGTCGAACTACATGGCCAAGCAGATTC
AGGCGGGCGTCTACCAGAAGCTCGACAAATCGAAGCTGCCGAACCTGTCGAACCTCGATCCGACGCTGATGAAGATGATC
GCCGACGCCGATCCGGGCAACCAGTACGGCGTGCCGTGGGCGTTCGGCACCGACGGCCTCGGCTACAACGTGCAGGCGGT
GAAGAAGGCGCTCGGCGAGAACGCGCCCGTCGACAGCTGGGCGCTCGTGCTCGATCCGGCCAACGTATCCAAGCTCAAGG
GCTGCGGCGTATCGTTCCTCGATCAGGCGGTCGACGTGTTCGCCGCGACGCTGCAATACATGGGCAGGAATCCGAACAGC
ACAAACCCGGCCGATTACCAGGCGGCGTTCGAGGTGCTGAAGAAAGTCCGCCCGTACATCACGCAATTCAACTCGTCCGG
CTACATCAACGATCTCGCGAACAACGACGTATGCGTCGCGCTCGGCTGGTCGGGCGACGTCGGCATCGCGCATCGCCGCT
CGGCCGAGGCGAAGCGCCCGTACGACATCAAGTTCTCGAATCCGAAGGAAGGCGGCCTGCTGTGGTTCGACGTGATGGTG
ATCCCGAAGGATGCGCCGCACGCGGAAGCCGCGCTGAAGTGGATCAACTACATCGAGGACCCGAAGGTCAACGCGGCGAT
CACCAACGAAGTGTTCTACCCGAGCGCGAACCGCGCGGCGCGACAGTTCGTCGTGCCCGCCGTCGCGCAGGATCCGACCG
TCTACCCGCCCGAGGACGTGCTCAAGAAGATGACGCTGATGCGGCCGATGCCCGCCGACATCCTGCGGCTCGAGAACCGT
CTGTGGGCGCAATTGAAGACGGGACACTGA

Upstream 100 bases:

>100_bases
GGCAACCTGATCATGCGCGCGACGGGCGACCGGATGCTGCTGTCGCCGCCGCTCGTGATCTCGAAACCGCAGATCGACGA
GATCGTCGAGAAGGCGAAGC

Downstream 100 bases:

>100_bases
TCACGGGCGGTTCCGGCGCGCGGCGATCGCCCGATTGACCGCCGCGCGCCCCGAGCAATTCGGCGCCGGCGCGTTCGACC
TGTCACGCGCGGCGCCTCGA

Product: putrescine ABC transporter periplasmic putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRSMRRRSNWVFPNGIANRAGGARAPRAHLHHQGNNMSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDY
IAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMI
ADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNS
TNPADYQAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMV
IPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENR
LWAQLKTGH

Sequences:

>Translated_409_residues
MRSMRRRSNWVFPNGIANRAGGARAPRAHLHHQGNNMSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDY
IAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMI
ADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNS
TNPADYQAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMV
IPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENR
LWAQLKTGH
>Mature_409_residues
MRSMRRRSNWVFPNGIANRAGGARAPRAHLHHQGNNMSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDY
IAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMI
ADADPGNQYGVPWAFGTDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNS
TNPADYQAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWFDVMV
IPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENR
LWAQLKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=47.9564032697548, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=36.5325077399381, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787711, Length=333, Percent_Identity=25.2252252252252, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44759; Mature: 44759

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00579 RIBOSOMAL_L29

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMRRRSNWVFPNGIANRAGGARAPRAHLHHQGNNMSVSHLRHAVARAALVALAGASAL
CCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
ALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGSSGYD
HCCHHHHCCCEEEEECCHHHHHHCCCCCCHHCCCCEEEECCCCCCCCEEEEEEECCCCCE
IVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFGTDGL
EEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCC
GYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNS
CCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TNPADYQAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCCCCCCCCCCCCCCC
YDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAA
EEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCEECCCCCHHH
RQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENRLWAQLKTGH
HHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSMRRRSNWVFPNGIANRAGGARAPRAHLHHQGNNMSVSHLRHAVARAALVALAGASAL
CCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
ALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDDTLQAKLLAGSSGYD
HCCHHHHCCCEEEEECCHHHHHHCCCCCCHHCCCCEEEECCCCCCCCEEEEEEECCCCCE
IVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFGTDGL
EEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCC
GYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNS
CCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TNPADYQAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCCCCCCCCCCCCCCC
YDIKFSNPKEGGLLWFDVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAA
EEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCEECCCCCHHH
RQFVVPAVAQDPTVYPPEDVLKKMTLMRPMPADILRLENRLWAQLKTGH
HHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]