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The map label for this gene is dhbE [H]

Identifier: 126444853

GI number: 126444853

Start: 848201

End: 849844

Strand: Reverse

Name: dhbE [H]

Synonym: BURPS668_A0874

Alternate gene names: 126444853

Gene position: 849844-848201 (Counterclockwise)

Preceding gene: 126444420

Following gene: 126443306

Centisome position: 27.17

GC content: 72.26

Gene sequence:

>1644_bases
TTGCCCGCCGCCGCGCGCAACGCCGCGTCGAGCGACGCGCCCGACTGGCCCGACGACTTCGCGCGCCGCTACCGCGACGC
CGGCTACTGGCGAGACGAGACCTTCTACGGCGCGCTCGCCGAGCGCGCGCGGCGCAGCCCCACCGCGACCGCGGTCGTCG
ACGGCGAGCGCCGCGTCAGCTACCGCGCGCTGCTCGAGCGCATCCGGCGTCTCGCCGGCGGCTTGCGCCGCCTGGGCCTC
GCGCGCGGCGACACGGCCGTCGTGCATCTGCCGAACGGCGTGCGCTTCATCGAAGCGTGCTTCGCGCTGTTCCAGCTCGG
CGTGCGCCCGGTGCTCGCGCTGCCCGCGCATCGGCAACACGAGATCGGCGCGTTCTGCCGCTTCACGAATGCCCGCGCAT
ATCTCGGCGCGGCCCGGCTCGGCGAGTTCGACTGCCGCCCGCTCGCTCACGCGCTGCGGGCGAGCTGCCCGGCGCTCGCG
CACATCGTCGTCGCGGGCGACGATCATGCCTTCTTGCATTTCGATTCGCTGTACGACACGCCGCCCGTCGCCGACTGCGC
GGCGCGCGCGGACGACATCGCGTGCTTCCAGCTCTCGGGCGGCACGACGGGCACGCCGAAACTGATTCCGCGCCGCCATC
GCGAGTACCTGTACAACGTGCGCGCGTGCTCGGACGCGAGCGGCTTCGGCGCCGACACCGTCTATCTGGCCGCGCTGCCG
ATGGCGCACAACTTCACGCTGTGCTGCCCCGGCGTGATCGGCGCGCTGCTCGCGGGCGGCCGCGTCGTCGCGACCGAGCA
CCCCGAGCCGGAGTGCGGCTTTGCGCTGATCGCGCGCGAGCGGGTCACGCATACGGCGCTCGTCCCGCCGCTCGCGCTAC
TGTGGCTGGACGCGCAGCGCGAGCGCCGAGCGGACCTGTCGAGCCTGCGCGTGCTGCAGGTCGGCGGCGCGCGGCTGATG
GACCACGCGGCCGAACGCGTGACGCCCGTCCTCGGCTGCCGGCTGCAACAGGTATTCGGAATGGCCGAAGGCCTCATCTG
CTGCACGCGGCTCGACGATCCGCCCGCGCGCATCGCGCGTACGCAGGGCCGGCCGGTGTCGCCCGCCGACGAAGTGCGCA
TCGTCGACGAGGCGGGCCGCGCCGTCGCGCCTGGCGAGATCGGCGAATTGCAGGTGCGCGGGCCCTATACGATCCGCGGC
TACTACCGGCTCGCCGAGCATCATGCGGCCGCGTTCACGGCCGACGGCTTCTATCGCACCGGCGACCGCGTGCGCCGCAC
CGAAGAAGGCGACCTCGTCGTCGAGGGCCGCGACAAGGACCAGATCAATCGCGGCGGCGAAAAAGTATCCGCCGAGGAAG
TCGAGAACCTGCTGCTCGCGCATTCGCAAATCCGCGACGCGGCGCTCGTCGCGATGTCCGATCCGCTGCTCGGCGAGCGC
ACGTGCGCGTTCGTCGTCGCGCGCCCGCCCGCGCCGACCTCGCTCGCGCTGAAACGGCATTTGCGCGACCACGGGCTCGC
CGCGTTCAAGATTCCCGATCGCATCGAATTCGTGCCGAGCTTTCCGGAAACCGGCATCGGCAAGACCAGCAAGAAATCGC
TGCGCGACCTGCTGCGCCGCCGGCTCGAAGCGGCGCGCGCATGA

Upstream 100 bases:

>100_bases
CGATTCGCCGGCCGCGTCTCCCACGGATTCGCCCGCCTGCGCCCCCAACGTTTCTCCGAACGTTTCCCCAAACGTTTCTC
CCGCCGGCTCGCCGTCGCCG

Downstream 100 bases:

>100_bases
AGCCGCCGCCTCCGCACGCCGGCTGGGTTCGCGAACTCAGGCTGTCGCCCTGCCCGCGCGCGCAGCTCGTCTGCATGCCG
CACGCGGGCGGCGCCGCAAG

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MPAAARNAASSDAPDWPDDFARRYRDAGYWRDETFYGALAERARRSPTATAVVDGERRVSYRALLERIRRLAGGLRRLGL
ARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDCRPLAHALRASCPALA
HIVVAGDDHAFLHFDSLYDTPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLM
DHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRG
YYRLAEHHAAAFTADGFYRTGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMSDPLLGER
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA

Sequences:

>Translated_547_residues
MPAAARNAASSDAPDWPDDFARRYRDAGYWRDETFYGALAERARRSPTATAVVDGERRVSYRALLERIRRLAGGLRRLGL
ARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDCRPLAHALRASCPALA
HIVVAGDDHAFLHFDSLYDTPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLM
DHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRG
YYRLAEHHAAAFTADGFYRTGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMSDPLLGER
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA
>Mature_546_residues
PAAARNAASSDAPDWPDDFARRYRDAGYWRDETFYGALAERARRSPTATAVVDGERRVSYRALLERIRRLAGGLRRLGLA
RGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDCRPLAHALRASCPALAH
IVVAGDDHAFLHFDSLYDTPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALPM
AHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLMD
HAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRGY
YRLAEHHAAAFTADGFYRTGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMSDPLLGERT
CAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=25.4716981132075, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI156151445, Length=563, Percent_Identity=23.0905861456483, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI115511026, Length=570, Percent_Identity=24.7368421052632, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI187761345, Length=537, Percent_Identity=27.3743016759777, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761343, Length=537, Percent_Identity=27.3743016759777, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=25.3411306042885, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI28416953, Length=561, Percent_Identity=24.5989304812834, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI157311624, Length=539, Percent_Identity=23.3766233766234, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI157311622, Length=539, Percent_Identity=23.3766233766234, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=22.735674676525, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI42544132, Length=547, Percent_Identity=22.1206581352834, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI8923896, Length=546, Percent_Identity=22.1611721611722, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=45.3183520599251, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI145693145, Length=531, Percent_Identity=30.6967984934087, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=24.8188405797101, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=27.3267326732673, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789201, Length=369, Percent_Identity=26.0162601626016, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786529, Length=576, Percent_Identity=23.6111111111111, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788595, Length=496, Percent_Identity=24.7983870967742, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=22.4761904761905, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786801, Length=463, Percent_Identity=23.110151187905, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=555, Percent_Identity=27.2072072072072, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=371, Percent_Identity=29.3800539083558, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=542, Percent_Identity=25.6457564575646, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=25.8126195028681, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=377, Percent_Identity=28.1167108753316, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=377, Percent_Identity=28.1167108753316, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=377, Percent_Identity=28.1167108753316, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=367, Percent_Identity=26.4305177111717, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=506, Percent_Identity=22.5296442687747, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=350, Percent_Identity=25.7142857142857, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507435, Length=530, Percent_Identity=24.7169811320755, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=311, Percent_Identity=26.6881028938907, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=26.536312849162, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983001, Length=543, Percent_Identity=23.7569060773481, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=543, Percent_Identity=23.7569060773481, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556356, Length=356, Percent_Identity=24.438202247191, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=524, Percent_Identity=23.2824427480916, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=25.9398496240602, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21355181, Length=508, Percent_Identity=23.8188976377953, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=24.6464646464646, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=24, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24656500, Length=500, Percent_Identity=24.6, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24667955, Length=548, Percent_Identity=22.8102189781022, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62472339, Length=548, Percent_Identity=22.8102189781022, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648260, Length=360, Percent_Identity=23.0555555555556, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356947, Length=351, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653035, Length=360, Percent_Identity=22.5, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667959, Length=432, Percent_Identity=23.3796296296296, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 59787; Mature: 59656

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAARNAASSDAPDWPDDFARRYRDAGYWRDETFYGALAERARRSPTATAVVDGERRVS
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHH
YRALLERIRRLAGGLRRLGLARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
EIGAFCRFTNARAYLGAARLGEFDCRPLAHALRASCPALAHIVVAGDDHAFLHFDSLYDT
CHHHHEEECCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCC
PPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
CCHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQR
CCCCCEEECHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERRADLSSLRVLQVGGARLMDHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIAR
HHHCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCCCHHHHHH
TQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRGYYRLAEHHAAAFTADGFYRT
CCCCCCCCHHHEEEHHHCCCEECCCCCCEEEECCCEEEHHHHHHHHHHHHEEECCCCCCC
GDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMSDPLLGER
CHHHHCCCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRR
EEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
RLEAARA
HHHHHCC
>Mature Secondary Structure 
PAAARNAASSDAPDWPDDFARRYRDAGYWRDETFYGALAERARRSPTATAVVDGERRVS
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHH
YRALLERIRRLAGGLRRLGLARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
EIGAFCRFTNARAYLGAARLGEFDCRPLAHALRASCPALAHIVVAGDDHAFLHFDSLYDT
CHHHHEEECCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCC
PPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
CCHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQR
CCCCCEEECHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERRADLSSLRVLQVGGARLMDHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIAR
HHHCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCCCHHHHHH
TQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRGYYRLAEHHAAAFTADGFYRT
CCCCCCCCHHHEEEHHHCCCEECCCCCCEEEECCCEEEHHHHHHHHHHHHEEECCCCCCC
GDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMSDPLLGER
CHHHHCCCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRR
EEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
RLEAARA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]