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The map label for this gene is fadD26 [H]

Identifier: 126444233

GI number: 126444233

Start: 1607775

End: 1609592

Strand: Reverse

Name: fadD26 [H]

Synonym: BURPS668_A1670

Alternate gene names: 126444233

Gene position: 1609592-1607775 (Counterclockwise)

Preceding gene: 126443369

Following gene: 126445348

Centisome position: 51.47

GC content: 75.58

Gene sequence:

>1818_bases
ATGACGGCGATCGTTCCCGCCGCCGCGGCGGCAGCGACGATGGTCGACATCGTGCGCGCGCGCGCCGACCGGCAGCCCGA
TCGGCCCGCGTACACGTATCTGCCGGAGCAGGAGGGCGCGCCGGCCGAGCTGCTCGACTACGCGAGCCTCGTGCAGCGCG
CGCACGCGATCGCGGCCGCGCTCGACAGGCGCTTGGCCGGCGCGCCCGACGCGCCGCGCATGGCGATGCTGCTGTTTGCG
CCGGGGCCGGATTTTCTCGCGGCGTTCTGGGGCTGCCTGTCGGCGCGCGTGATCGCGATTCCGGCGCAGTTGCCGCGCCC
CGGGCGCTGCGCCGCGACGCTCGAGGCGATCGTGCGCAACGCGGGCGTGCGGCTGGTACTCGCTAGCCGGCCGCAGGCCG
ACGCGATCGGCCGGACGCTCGCGCGCTCCCCGGCGCTCGCGGGGCTCGACACGCTGTTCGTCGACGACGCGCTCGCCGAC
GGCGCCGGGCGCCCCGCGCCGGCGCAGCCCGCGGCGGACGACATCGCGTTCCTGCAATACACGTCGGGCTCGACGAGCCG
GCCGAAGGGCGTCGTCGTCCGTCACCGGAACCTTGTCGCCAACGAACGGATGATCGCGCAGGCGATGTCGCTCGATCACG
CGTCGACCTCCGTGATGTGGATGCCGCACTACCACGACATGGGCCTGATCGGCGGCATGCTGCAGCCGCTCTACAGCGGC
GCGCACTGCGTCGCGATGGCGCCGACGACGTTCCTGAAGCGGCCGCTGCGCTGGCTGCGGGCGATCGCGCAATGGCGCGC
GGTGTCGTCCGGCGGGCCGGATTTCGCGTACCGGCTGTGCGTCGAGCGGATCGCGCCCGAGCAGGCGGCCGGGCTCGACC
TGTCGAGCTGGCGCGTCGCGTTCAACGGCGCGGAGCCCGTGCGCGCGGCGACGATCGCCGCGTTCGCCGAGCGCTTCGGG
CCCGCCGGCTTTCGCGCGCACGCGAGCTATCCGTGCTACGGCATGGCGGAAGCGACGCTGCTCGCCGCGGGCGGGCGCGC
CGGCGACGGCGCGCACGTGCGGCGGATCTCGGCCGCGGCGCTGGCGGCCGGCCGCGCCGAGCCGCCGGCGCCGGGCGACG
CGGGCACGGCCGCCGTGTGCTGCGGCGCGCCGCCCGCGGGCGCGCGACTCGCCGTCGTCGATCCGGCGACGCGGCAAAGG
CGCGCCGACGGGCTCGTCGGCGAGCTCTGGATCGCCGGCCCGCACGTCGCCGACGGCTATCATCGCGCGCCCGAGGCGAG
CGCCGCGGCGTTCGGCGCCGCGATCGCCGGCCCCGAGCCGGACGCGGGCCCGTGGCTGCGCACGGGCGATCTCGGCTTCG
TGCTCGACGGCGGCGTGTACGTCACCGGCCGGCTGAAGGAGATGATGATCGTCAACGGGCGCAATCTGTATCCGCACGAT
GTCGAGGACACGCTGCGCGAGGGGCTCGCCGAGATTCGCGACGCCGCGGTGTTCGCGCTGATCGAACCGGGCGGTCGCGA
GCGCACGGTCGCGATCCTCGAGCTGGCCGCCGCGCAGCGCCGGCTGATCCTGCAACCGGACGCGCATGCCGACGCGGGGC
TCCAGGCGATCGCCATGGACGCACGCGCGCTCGCCGCGCGCGCGTGCGAGCTGCCGCTCGACCACGTGCGGCTGAGCCTG
CCGGGGGCGATCGTCAAGACGACGAGCGGCAAGACGCGCTATGGCGAGCTGCGCGCGCGCATGCTCGCGCTGCCGGAATC
GCTGCGGCAGGCGCCGGTATCGATCGAGGCGGGCCGCGCCCGCCGGGAGGAAGCATGA

Upstream 100 bases:

>100_bases
AAGTCATCCTGCTGCATTTGTGGCACGACCCCGCGCGCCGTGTCGACGCGATCGAGGCGATGGCGTCGCTGATTCATCCG
TTAGCCGAACCGGTGCGCGC

Downstream 100 bases:

>100_bases
ACACGACGAACACGGCCTGCGCCCCCACGGCGGAGGCGATCGAGCGTTGGCTGATCGGGCGGATCGCGGCGGCGACGGGC
GGCGAGGCGGCCGCGATCGA

Product: AMP-binding domain-containing protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MTAIVPAAAAAATMVDIVRARADRQPDRPAYTYLPEQEGAPAELLDYASLVQRAHAIAAALDRRLAGAPDAPRMAMLLFA
PGPDFLAAFWGCLSARVIAIPAQLPRPGRCAATLEAIVRNAGVRLVLASRPQADAIGRTLARSPALAGLDTLFVDDALAD
GAGRPAPAQPAADDIAFLQYTSGSTSRPKGVVVRHRNLVANERMIAQAMSLDHASTSVMWMPHYHDMGLIGGMLQPLYSG
AHCVAMAPTTFLKRPLRWLRAIAQWRAVSSGGPDFAYRLCVERIAPEQAAGLDLSSWRVAFNGAEPVRAATIAAFAERFG
PAGFRAHASYPCYGMAEATLLAAGGRAGDGAHVRRISAAALAAGRAEPPAPGDAGTAAVCCGAPPAGARLAVVDPATRQR
RADGLVGELWIAGPHVADGYHRAPEASAAAFGAAIAGPEPDAGPWLRTGDLGFVLDGGVYVTGRLKEMMIVNGRNLYPHD
VEDTLREGLAEIRDAAVFALIEPGGRERTVAILELAAAQRRLILQPDAHADAGLQAIAMDARALAARACELPLDHVRLSL
PGAIVKTTSGKTRYGELRARMLALPESLRQAPVSIEAGRARREEA

Sequences:

>Translated_605_residues
MTAIVPAAAAAATMVDIVRARADRQPDRPAYTYLPEQEGAPAELLDYASLVQRAHAIAAALDRRLAGAPDAPRMAMLLFA
PGPDFLAAFWGCLSARVIAIPAQLPRPGRCAATLEAIVRNAGVRLVLASRPQADAIGRTLARSPALAGLDTLFVDDALAD
GAGRPAPAQPAADDIAFLQYTSGSTSRPKGVVVRHRNLVANERMIAQAMSLDHASTSVMWMPHYHDMGLIGGMLQPLYSG
AHCVAMAPTTFLKRPLRWLRAIAQWRAVSSGGPDFAYRLCVERIAPEQAAGLDLSSWRVAFNGAEPVRAATIAAFAERFG
PAGFRAHASYPCYGMAEATLLAAGGRAGDGAHVRRISAAALAAGRAEPPAPGDAGTAAVCCGAPPAGARLAVVDPATRQR
RADGLVGELWIAGPHVADGYHRAPEASAAAFGAAIAGPEPDAGPWLRTGDLGFVLDGGVYVTGRLKEMMIVNGRNLYPHD
VEDTLREGLAEIRDAAVFALIEPGGRERTVAILELAAAQRRLILQPDAHADAGLQAIAMDARALAARACELPLDHVRLSL
PGAIVKTTSGKTRYGELRARMLALPESLRQAPVSIEAGRARREEA
>Mature_604_residues
TAIVPAAAAAATMVDIVRARADRQPDRPAYTYLPEQEGAPAELLDYASLVQRAHAIAAALDRRLAGAPDAPRMAMLLFAP
GPDFLAAFWGCLSARVIAIPAQLPRPGRCAATLEAIVRNAGVRLVLASRPQADAIGRTLARSPALAGLDTLFVDDALADG
AGRPAPAQPAADDIAFLQYTSGSTSRPKGVVVRHRNLVANERMIAQAMSLDHASTSVMWMPHYHDMGLIGGMLQPLYSGA
HCVAMAPTTFLKRPLRWLRAIAQWRAVSSGGPDFAYRLCVERIAPEQAAGLDLSSWRVAFNGAEPVRAATIAAFAERFGP
AGFRAHASYPCYGMAEATLLAAGGRAGDGAHVRRISAAALAAGRAEPPAPGDAGTAAVCCGAPPAGARLAVVDPATRQRR
ADGLVGELWIAGPHVADGYHRAPEASAAAFGAAIAGPEPDAGPWLRTGDLGFVLDGGVYVTGRLKEMMIVNGRNLYPHDV
EDTLREGLAEIRDAAVFALIEPGGRERTVAILELAAAQRRLILQPDAHADAGLQAIAMDARALAARACELPLDHVRLSLP
GAIVKTTSGKTRYGELRARMLALPESLRQAPVSIEAGRARREEA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=518, Percent_Identity=25.4826254826255, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI45827692, Length=546, Percent_Identity=24.5421245421245, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI225735629, Length=546, Percent_Identity=24.5421245421245, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI55749758, Length=536, Percent_Identity=23.3208955223881, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI225735625, Length=503, Percent_Identity=25.6461232604374, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45827694, Length=500, Percent_Identity=25.6, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI225735627, Length=500, Percent_Identity=25.6, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI45827696, Length=500, Percent_Identity=25.6, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI45827698, Length=342, Percent_Identity=26.0233918128655, Blast_Score=80, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193202679, Length=532, Percent_Identity=24.0601503759398, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=347, Percent_Identity=25.0720461095101, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654656, Length=470, Percent_Identity=25.3191489361702, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859661, Length=341, Percent_Identity=27.5659824046921, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63580; Mature: 63448

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVPAAAAAATMVDIVRARADRQPDRPAYTYLPEQEGAPAELLDYASLVQRAHAIAAA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LDRRLAGAPDAPRMAMLLFAPGPDFLAAFWGCLSARVIAIPAQLPRPGRCAATLEAIVRN
HHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHC
AGVRLVLASRPQADAIGRTLARSPALAGLDTLFVDDALADGAGRPAPAQPAADDIAFLQY
CCEEEEEECCCCHHHHHHHHHCCCCHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEE
TSGSTSRPKGVVVRHRNLVANERMIAQAMSLDHASTSVMWMPHYHDMGLIGGMLQPLYSG
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCC
AHCVAMAPTTFLKRPLRWLRAIAQWRAVSSGGPDFAYRLCVERIAPEQAAGLDLSSWRVA
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCCCHHCEEE
FNGAEPVRAATIAAFAERFGPAGFRAHASYPCYGMAEATLLAAGGRAGDGAHVRRISAAA
ECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHH
LAAGRAEPPAPGDAGTAAVCCGAPPAGARLAVVDPATRQRRADGLVGELWIAGPHVADGY
HHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCC
HRAPEASAAAFGAAIAGPEPDAGPWLRTGDLGFVLDGGVYVTGRLKEMMIVNGRNLYPHD
CCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCEEEECCEEEEECCEEEEEECCCCCCCCC
VEDTLREGLAEIRDAAVFALIEPGGRERTVAILELAAAQRRLILQPDAHADAGLQAIAMD
HHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHH
ARALAARACELPLDHVRLSLPGAIVKTTSGKTRYGELRARMLALPESLRQAPVSIEAGRA
HHHHHHHHHCCCHHHEEEECCCEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCEEECCCC
RREEA
CCCCC
>Mature Secondary Structure 
TAIVPAAAAAATMVDIVRARADRQPDRPAYTYLPEQEGAPAELLDYASLVQRAHAIAAA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LDRRLAGAPDAPRMAMLLFAPGPDFLAAFWGCLSARVIAIPAQLPRPGRCAATLEAIVRN
HHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHC
AGVRLVLASRPQADAIGRTLARSPALAGLDTLFVDDALADGAGRPAPAQPAADDIAFLQY
CCEEEEEECCCCHHHHHHHHHCCCCHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEE
TSGSTSRPKGVVVRHRNLVANERMIAQAMSLDHASTSVMWMPHYHDMGLIGGMLQPLYSG
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCC
AHCVAMAPTTFLKRPLRWLRAIAQWRAVSSGGPDFAYRLCVERIAPEQAAGLDLSSWRVA
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCCCHHCEEE
FNGAEPVRAATIAAFAERFGPAGFRAHASYPCYGMAEATLLAAGGRAGDGAHVRRISAAA
ECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHH
LAAGRAEPPAPGDAGTAAVCCGAPPAGARLAVVDPATRQRRADGLVGELWIAGPHVADGY
HHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCC
HRAPEASAAAFGAAIAGPEPDAGPWLRTGDLGFVLDGGVYVTGRLKEMMIVNGRNLYPHD
CCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCEEEECCEEEEECCEEEEEECCCCCCCCC
VEDTLREGLAEIRDAAVFALIEPGGRERTVAILELAAAQRRLILQPDAHADAGLQAIAMD
HHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHH
ARALAARACELPLDHVRLSLPGAIVKTTSGKTRYGELRARMLALPESLRQAPVSIEAGRA
HHHHHHHHHCCCHHHEEEECCCEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCEEECCCC
RREEA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]