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The map label for this gene is poxB [H]

Identifier: 126444020

GI number: 126444020

Start: 2243659

End: 2245377

Strand: Reverse

Name: poxB [H]

Synonym: BURPS668_A2302

Alternate gene names: 126444020

Gene position: 2245377-2243659 (Counterclockwise)

Preceding gene: 126445028

Following gene: 126442336

Centisome position: 71.8

GC content: 68.35

Gene sequence:

>1719_bases
ATGGCGAGCACGATTGCCGACTACCTCGCGCGGACGCTGGCCGCCGCCGGCGTCACGAAGATCTGGGGCGTGACGGGCGA
CAGCCTGAACGGATTGTCCGACAGCCTGCGCCGCGACGGCGGCATTCGCTGGATGCATACCCGGCATGAAGAGGTGGCGG
CGTTCGCCGCGGGCGCCGAAGCGGCGCAGACCGGCCGGCTGGCCGTATGCGCGGGCAGTTGCGGGCCGGGCAATCTGCAT
CTGATCAACGGCTTGTTCGATTGCCATCGCAATCATGTGCCGGTGCTCGCGATCGCCGCGCAGATTTCGTCGACCGAAAT
CGGGCTCGGCTATTTTCAGGAAACGCATCCGCAGGAACTGTTTCGCGAATGCAGCCATTACGTCGAGCTCGTGACGAACG
CGTCGCAATTTCCGCGCGTGCTCGAGCGCGCGATGCGCGCCGCGATCGGCGAGCACGGCGTCGCGGTGATCGTGCTCCCG
GGCGATGTCGCGCTGAGCGCGGCGCCCGCGCGAACACCGCCGTGGTTCGATTTCACCTCGCCGGTGACGACGCCCGCCGA
CGCCGATCTGGAGCGGCTCGCGGCGTTGCTCGACGGCTCCGATGCAGTCACGCTGCTGTGCGGCAGCGGTTGCGCGGGCG
CGCACGACGAAGTCGTCGCGTTGGCCGATGCGCTCGCGGCACCCGTCGTGCATGCGCTGCGCGGCAAGCAGCACATTGAA
TACGACAATCCGAACAGCGTCGGCATGACGGGGCTGATCGGCTTCAGCTCCGGCTATCACGCGATGCAATCGTGCGACAC
GCTGCTGATGCTCGGCACCGATTTCCCCTATCGCAATTTCTATCCGACGCGCGCCCGGATCGCGCAGATCGACCGCCGCG
CGTCGGCGCTCGGCCGTCGCGCGCCGCTCGACGTCGGCCTCGTCGGCGACGTGCGCGCCACGCTGTCCGCGCTGTTGCCG
CGCATCCGGCGCAAGACGCGGCGCGGTTTCGTCGAGCGTGCGCTTGCGCATTATGCCGCCGCGCGCAAGGGGCTCGACGA
ACTGGCGAGACCGTCGCCGGCCGGGCGGCCGATTCATCCGCAGTACTTGACGCGCGTCGTCGACGAGCTCGCCGCCGACG
ATGCGAACTTCGCCGCGGACGTCGGCACGCCGACGCTGTGGGCGGCGCGCTATCTGACGATGAACGGCCGCAGGCGGCTG
CACGGATCGTTCAATCACGGCTCGATGGCGAACGCGTTGCCGCAGGCGCTGGGCGCGCAGGCCGCGCATCCCGGCCGGCA
AGTCGTATCGCTGTCGGGCGACGGCGGGCTGTCGATGCTCCTCGGCGATCTGCTGAGCGCGCGGCAACTCGATTTGCCGA
TCAAGGTGATCGTCTACAACAACAGCTCGCTGGGCTTCGTCGCGATGGAGATGAAGGCGGGCGGTTATCTGGATACGGGC
ACCGATCTGCACGAGACCGATTTCGCGGCGATCGCGAAGGGGGCGGGCCTCTACAGCCTGCGCGTGACCGAATCGGAAGC
GTTGCCCGATGCGCTCGGCAGGCTGCTCGCGCACCCGGGGCCCGCGCTGCTCGACGTCGTCACCGACAAGCACGAGCTCG
CGATGCCGCCGAAGATCGAATGGGCGCAGGCGAAGGGATTCAGCCTCTACATGTTGCGCGCGGTGCTGAGCGGGCGCGGC
GACGAAGTGCTCGAGCTCGCGCAGACGAATCTGCGGTGA

Upstream 100 bases:

>100_bases
CGAACGGGCGCGCGCGCTGATCCGGCTCGCGCACCCCGATTTTCGCGACGCGCTGAGCGTGCACGCACGCCGGCTACACT
TGATCTGAATGGAGGATCGC

Downstream 100 bases:

>100_bases
AGGCACGGGGGTGGCCGCGCGATGCGCCGCCCCATTGAACGCGGCATCGCGAATGGCTACCGGCAGCGCGCCGCGCCTCG
TCGACCCAGCCCGCCCGCGT

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAEAAQTGRLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAQISSTEIGLGYFQETHPQELFRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLP
GDVALSAAPARTPPWFDFTSPVTTPADADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRRAPLDVGLVGDVRATLSALLP
RIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHPQYLTRVVDELAADDANFAADVGTPTLWAARYLTMNGRRRL
HGSFNHGSMANALPQALGAQAAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGRLLAHPGPALLDVVTDKHELAMPPKIEWAQAKGFSLYMLRAVLSGRG
DEVLELAQTNLR

Sequences:

>Translated_572_residues
MASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAEAAQTGRLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAQISSTEIGLGYFQETHPQELFRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLP
GDVALSAAPARTPPWFDFTSPVTTPADADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRRAPLDVGLVGDVRATLSALLP
RIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHPQYLTRVVDELAADDANFAADVGTPTLWAARYLTMNGRRRL
HGSFNHGSMANALPQALGAQAAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGRLLAHPGPALLDVVTDKHELAMPPKIEWAQAKGFSLYMLRAVLSGRG
DEVLELAQTNLR
>Mature_571_residues
ASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAEAAQTGRLAVCAGSCGPGNLHL
INGLFDCHRNHVPVLAIAAQISSTEIGLGYFQETHPQELFRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLPG
DVALSAAPARTPPWFDFTSPVTTPADADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIEY
DNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRRAPLDVGLVGDVRATLSALLPR
IRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHPQYLTRVVDELAADDANFAADVGTPTLWAARYLTMNGRRRLH
GSFNHGSMANALPQALGAQAAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTGT
DLHETDFAAIAKGAGLYSLRVTESEALPDALGRLLAHPGPALLDVVTDKHELAMPPKIEWAQAKGFSLYMLRAVLSGRGD
EVLELAQTNLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=488, Percent_Identity=23.5655737704918, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=64.4483362521891, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=28.1818181818182, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=26.3063063063063, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1786717, Length=550, Percent_Identity=25.0909090909091, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788716, Length=542, Percent_Identity=20.2952029520295, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17531299, Length=483, Percent_Identity=25.2587991718427, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=483, Percent_Identity=25.2587991718427, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=24.0213523131673, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=21.8623481781377, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=494, Percent_Identity=22.6720647773279, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=24.7401247401247, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 60942; Mature: 60811

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHCCH
AAQTGRLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAQISSTEIGLGYFQETHPQEL
HHCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCEEEEEEHHCCCCCCCCHHHCCCHHHH
FRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLPGDVALSAAPARTPPWFDFTS
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCCC
PVTTPADADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
CCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRR
CCCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
APLDVGLVGDVRATLSALLPRIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCH
QYLTRVVDELAADDANFAADVGTPTLWAARYLTMNGRRRLHGSFNHGSMANALPQALGAQ
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCC
AAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCC
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGRLLAHPGPALLDVVTDKHELAMPPKIE
CCCCHHHHHHHHCCCCEEEEEECCHHCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCC
WAQAKGFSLYMLRAVLSGRGDEVLELAQTNLR
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ASTIADYLARTLAAAGVTKIWGVTGDSLNGLSDSLRRDGGIRWMHTRHEEVAAFAAGAE
CHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHCCH
AAQTGRLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAQISSTEIGLGYFQETHPQEL
HHCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCEEEEEEHHCCCCCCCCHHHCCCHHHH
FRECSHYVELVTNASQFPRVLERAMRAAIGEHGVAVIVLPGDVALSAAPARTPPWFDFTS
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCCC
PVTTPADADLERLAALLDGSDAVTLLCGSGCAGAHDEVVALADALAAPVVHALRGKQHIE
CCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
YDNPNSVGMTGLIGFSSGYHAMQSCDTLLMLGTDFPYRNFYPTRARIAQIDRRASALGRR
CCCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
APLDVGLVGDVRATLSALLPRIRRKTRRGFVERALAHYAAARKGLDELARPSPAGRPIHP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCH
QYLTRVVDELAADDANFAADVGTPTLWAARYLTMNGRRRLHGSFNHGSMANALPQALGAQ
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHCCC
AAHPGRQVVSLSGDGGLSMLLGDLLSARQLDLPIKVIVYNNSSLGFVAMEMKAGGYLDTG
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCCCCCC
TDLHETDFAAIAKGAGLYSLRVTESEALPDALGRLLAHPGPALLDVVTDKHELAMPPKIE
CCCCHHHHHHHHCCCCEEEEEECCHHCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCC
WAQAKGFSLYMLRAVLSGRGDEVLELAQTNLR
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]