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The map label for this gene is citH [H]

Identifier: 126443821

GI number: 126443821

Start: 260994

End: 262307

Strand: Reverse

Name: citH [H]

Synonym: BURPS668_A0294

Alternate gene names: 126443821

Gene position: 262307-260994 (Counterclockwise)

Preceding gene: 126442979

Following gene: 126443052

Centisome position: 8.39

GC content: 71.31

Gene sequence:

>1314_bases
ATGGAAACGTCCGCTCTCAATTTCGCCGGCGCGCCGGCCGACGCGCGCGCCGCCGCAAAGAAACGCCGCCTGATCGCCGC
GGCCGCCGTCGGCAACGCGCTCGAGTTCTACGATTTCACCGTCTACAGCTTCTTCGCGATCCTGATCGGCAAGCTGTTCT
TCCCGGTTCACTCGCCGTTCGGGCAGCTGATGCTCGCGGTCGCGAGCTTCGGCGTGGGGTTCGTCACGCGCCCGCTCGGC
GGGCTCGTGATCGGCGTCTACGCGGATCGCGCCGGGCGCAAGCGCGCGATGATCGCAACGCTGCTCATCATGGCGCTCGG
CACCGCGACGATCGCCGTCGCGCCGACTTACGCGCAGATCGGCATCGCGGCGCCGCTGCTGCTCGTGGCCGCGCGCTTGC
TGCAGGGGTTCGCGTCCGGCGGCGAGGTCGGCGCGTCCACCACGCTGCTGATCGAGCAGGCGCCGCAGGCGCGGCGCGGC
TTCTATGCGTCGTTCCAGTTCTCGAGCCAGGGGCTCGCCGCGCTCGCCGGCGCGCTCACCGGCGTGCTGCTCACGTCGAC
GCTCAGCGCCGCGCAACTCGAGAGCTGGGGGTGGCGCGTGCCGTTCGTCATCGGCACGCTGTTCGCGCCGCTCGGCTACT
GGCTGCGGCGCACGGTCGACGAGGCGCCGGCGCCGGCGTCCGCGCCGCGCGGCGACGCCGCGCCGATGCCGCTCGCCGAC
GTGCTGCGCCATCACGGCAAGGCCGTGTTCGCCGGCCTCGGCATCACGATCGGCGGCACGTCGATCCACTACATCATCGT
GTTCTACATGGCGATCTACGGCGTGCAGGTGCTGCATCTGCCGAGCTGGCTGTCGATGTCGGCGGGCTGCATCGCCGGCG
CGATCCTCGCGGTGGTCACGCCGATCGGCGGCCATCTGTCGGACCGCTACGGGCGCAACCGGATCGTCTGGTGGACGCGC
GTCGCGCTGATGCTCGCGATCTATCCGGCGTTCGTCGCGCTCAACCGCTGGCCCGGCGCGGCTTCGCTGCTGTCGATCAT
CGCGGCGCTCGCGAGCGTGCATGCGATCAACATCGGCGCGACGGGCGCGATGCTCGGCGAGCTGTTCCCGCGCCCGGTGC
GCGCGACGGGCGGCGCGCTCGTCTACAGCGTCGGCGTCGCGATCTTCGGCGGATTCGCGCAATTCTTCGTCACGTGGCTG
ATCGCCGCGACGGGCAATGCGAACGCGCCCGCGTGGTACGCGATCGGCTGCGGCGCGCTGACGCTCGCCGCGCTCGGCTC
GATGGACGACAAGGCGGGCAAGCCGCTCGACTGA

Upstream 100 bases:

>100_bases
ATCCTCGCGATCGGCGCGAGCTATTGGGTGCGGCTTGCCGAAGCATGGCTCGCCGCCTGATGCGGCGCCGCCGCGCCGTC
TCGCGCTCCTCGGGGGTCAC

Downstream 100 bases:

>100_bases
CGCCGCGCGCCGCGGCGCGCGGCCGCGCATCGGCATGGCGGGCGGCTTCGCGAACGATCGCCTCTACGGCGCGGCGCTGC
AATACCAGCGCGGCGGCCTG

Product: major facilitator superfamily permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG
GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRG
FYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAPASAPRGDAAPMPLAD
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNRIVWWTR
VALMLAIYPAFVALNRWPGAASLLSIIAALASVHAINIGATGAMLGELFPRPVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPLD

Sequences:

>Translated_437_residues
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG
GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRG
FYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAPASAPRGDAAPMPLAD
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNRIVWWTR
VALMLAIYPAFVALNRWPGAASLLSIIAALASVHAINIGATGAMLGELFPRPVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPLD
>Mature_437_residues
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPFGQLMLAVASFGVGFVTRPLG
GLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQIGIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRG
FYASFQFSSQGLAALAGALTGVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAPASAPRGDAAPMPLAD
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVTPIGGHLSDRYGRNRIVWWTR
VALMLAIYPAFVALNRWPGAASLLSIIAALASVHAINIGATGAMLGELFPRPVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNANAPAWYAIGCGALTLAALGSMDDKAGKPLD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=437, Percent_Identity=30.8924485125858, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1788942, Length=408, Percent_Identity=32.843137254902, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=30.9692671394799, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789941, Length=420, Percent_Identity=31.9047619047619, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87082231, Length=236, Percent_Identity=29.2372881355932, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082404, Length=323, Percent_Identity=23.5294117647059, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45438; Mature: 45438

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GQLMLAVASFGVGFVTRPLGGLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQI
HHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEECCHHHHH
GIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRGFYASFQFSSQGLAALAGALT
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCEEEEEECCCCHHHHHHHHH
GVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAPASAPRGDAAPMPLAD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVT
HHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PIGGHLSDRYGRNRIVWWTRVALMLAIYPAFVALNRWPGAASLLSIIAALASVHAINIGA
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCC
TGAMLGELFPRPVRATGGALVYSVGVAIFGGFAQFFVTWLIAATGNANAPAWYAIGCGAL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
TLAALGSMDDKAGKPLD
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
METSALNFAGAPADARAAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSPF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
GQLMLAVASFGVGFVTRPLGGLVIGVYADRAGRKRAMIATLLIMALGTATIAVAPTYAQI
HHHHHHHHHHCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEECCHHHHH
GIAAPLLLVAARLLQGFASGGEVGASTTLLIEQAPQARRGFYASFQFSSQGLAALAGALT
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCEEEEEECCCCHHHHHHHHH
GVLLTSTLSAAQLESWGWRVPFVIGTLFAPLGYWLRRTVDEAPAPASAPRGDAAPMPLAD
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
VLRHHGKAVFAGLGITIGGTSIHYIIVFYMAIYGVQVLHLPSWLSMSAGCIAGAILAVVT
HHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PIGGHLSDRYGRNRIVWWTRVALMLAIYPAFVALNRWPGAASLLSIIAALASVHAINIGA
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCC
TGAMLGELFPRPVRATGGALVYSVGVAIFGGFAQFFVTWLIAATGNANAPAWYAIGCGAL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
TLAALGSMDDKAGKPLD
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]