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The map label for this gene is estB [H]

Identifier: 126443556

GI number: 126443556

Start: 1815453

End: 1816652

Strand: Reverse

Name: estB [H]

Synonym: BURPS668_A1860

Alternate gene names: 126443556

Gene position: 1816652-1815453 (Counterclockwise)

Preceding gene: 126443716

Following gene: 126442790

Centisome position: 58.09

GC content: 74.5

Gene sequence:

>1200_bases
ATGAATTCGGCATTCGATGCCCCTGCATCCGCGGCGGCCGCGGCCTTTCCCCCGGCGGACGACGCCGCGCTCGCCGCACG
GCTCGACCGCGTGCTCGGCGCGGCGCTCGGCGAGCGGCGCATCGTCGGCGCGGTCGCGCTCGTCGCGCGGCACGGCCGGC
TCGTCTACCGGCGCGCGCACGGTCTCGCGGAGCGCGAGACGCAGCGGCCGATGCGCGAGGACACGCTGTTTCGGCTGTCG
TCGATCACGAAGCCGATCGTCACCGTCGCGGTGCTGCGCCTCGTCGCCGACGGCCGAATGGCGCTCGACGCGCCGATCAC
GCGCTGGCTGCCCGATTTCGCGCCCGCGCTGCCCGACGGCCGGGCGCCCGCGCTGAGCGTGCATCAACTGCTGACCCACA
CGGCGGGCCTGAGCTACTGGCTGCTCGAAGCGCCCGGCTCGGCCTATCACACGCTCGGCGTCTCGGACGGCATCGATCTC
GTCGACTTCGATCTCGCGGAGAACCTGCGGCGCATCGCGGCCGCGCCGCTCGCGTTCGAGCCGGGCAGCGCGTGGCGCTA
TTCGCTCGCGCTCGACGTGCTCGGCGCGGCGATCGAGCGCGAGACGGGGCGCGCGCTGCCGGACGCGGTCGCGCGGCTCG
TCACGACGCCGCTCGGGATGCGCGACACGGGCTTCGTCGCGGCCGACCCCGCGCGTTTCGCGGTGGCGTACGCGAACGCC
GCGCCGGAGCCCGCGCGGATCACCGACAACCTCGACGTGCCGTTGCCCGAGGGGCACGGCGTCGCGGTGCGCTTCGCGCC
GTCGCGGGTGTTCGACGCGACGGCGTTCGCGTCGGGCGGCGCGGGGATGTACGGCAGCGCCGACGACGTGCTGCGCGTGC
TCGAGACGATCCGCACGGGCGGCGACGGTTTCCTGCCGGCCGCGCTCGTCGCCGCGATGCGCACCGATCACACGGGGCCG
GCCGCCGGCACGCGCGGGCCCGGCTGGGGCTTCGGCTATGGCGGCGCGGTGCTGTCCGATCCGGCGCTCGCGCAGTCGCC
GCAGAGCGCGGGAACCCTGCAATGGGGCGGCGTCTACGGGCATTCGTGGTTCGTCGACGCGGCGCGCGGGCTCACCGTGC
TGTTGATGACGAACACCGCGTATGAAGGGATGTCGGGGCCGTTGACGCTTGAGGTGCGCGACGCGGTGTACGGCGTGTGA

Upstream 100 bases:

>100_bases
GCAGCTTGCGCGCATCGCCCTGGCCCGGCGCGGGGCAACGCCGGCGGAATCGGCGCGGGCTGTCTAGAATCGACGTTTTG
AACCCGTGAGGATCCACACG

Downstream 100 bases:

>100_bases
CGCACGGCGTCGCCCCGGCGCGTCGTGCCGGGGCGACGCAGGCCGACGCAGGCCGCGCGCCACCGGATGCGGCGGGCACG
GGCCGTCGCGTGCCGCGCGG

Product: putative esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MNSAFDAPASAAAAAFPPADDAALAARLDRVLGAALGERRIVGAVALVARHGRLVYRRAHGLAERETQRPMREDTLFRLS
SITKPIVTVAVLRLVADGRMALDAPITRWLPDFAPALPDGRAPALSVHQLLTHTAGLSYWLLEAPGSAYHTLGVSDGIDL
VDFDLAENLRRIAAAPLAFEPGSAWRYSLALDVLGAAIERETGRALPDAVARLVTTPLGMRDTGFVAADPARFAVAYANA
APEPARITDNLDVPLPEGHGVAVRFAPSRVFDATAFASGGAGMYGSADDVLRVLETIRTGGDGFLPAALVAAMRTDHTGP
AAGTRGPGWGFGYGGAVLSDPALAQSPQSAGTLQWGGVYGHSWFVDAARGLTVLLMTNTAYEGMSGPLTLEVRDAVYGV

Sequences:

>Translated_399_residues
MNSAFDAPASAAAAAFPPADDAALAARLDRVLGAALGERRIVGAVALVARHGRLVYRRAHGLAERETQRPMREDTLFRLS
SITKPIVTVAVLRLVADGRMALDAPITRWLPDFAPALPDGRAPALSVHQLLTHTAGLSYWLLEAPGSAYHTLGVSDGIDL
VDFDLAENLRRIAAAPLAFEPGSAWRYSLALDVLGAAIERETGRALPDAVARLVTTPLGMRDTGFVAADPARFAVAYANA
APEPARITDNLDVPLPEGHGVAVRFAPSRVFDATAFASGGAGMYGSADDVLRVLETIRTGGDGFLPAALVAAMRTDHTGP
AAGTRGPGWGFGYGGAVLSDPALAQSPQSAGTLQWGGVYGHSWFVDAARGLTVLLMTNTAYEGMSGPLTLEVRDAVYGV
>Mature_399_residues
MNSAFDAPASAAAAAFPPADDAALAARLDRVLGAALGERRIVGAVALVARHGRLVYRRAHGLAERETQRPMREDTLFRLS
SITKPIVTVAVLRLVADGRMALDAPITRWLPDFAPALPDGRAPALSVHQLLTHTAGLSYWLLEAPGSAYHTLGVSDGIDL
VDFDLAENLRRIAAAPLAFEPGSAWRYSLALDVLGAAIERETGRALPDAVARLVTTPLGMRDTGFVAADPARFAVAYANA
APEPARITDNLDVPLPEGHGVAVRFAPSRVFDATAFASGGAGMYGSADDVLRVLETIRTGGDGFLPAALVAAMRTDHTGP
AAGTRGPGWGFGYGGAVLSDPALAQSPQSAGTLQWGGVYGHSWFVDAARGLTVLLMTNTAYEGMSGPLTLEVRDAVYGV

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=367, Percent_Identity=25.3405994550409, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533026, Length=184, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 41711; Mature: 41711

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAFDAPASAAAAAFPPADDAALAARLDRVLGAALGERRIVGAVALVARHGRLVYRRAH
CCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEEHHHH
GLAERETQRPMREDTLFRLSSITKPIVTVAVLRLVADGRMALDAPITRWLPDFAPALPDG
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCC
RAPALSVHQLLTHTAGLSYWLLEAPGSAYHTLGVSDGIDLVDFDLAENLRRIAAAPLAFE
CCCHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCCEEEHHHHHHHHHHHHCCCEEC
PGSAWRYSLALDVLGAAIERETGRALPDAVARLVTTPLGMRDTGFVAADPARFAVAYANA
CCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHEEEEECCC
APEPARITDNLDVPLPEGHGVAVRFAPSRVFDATAFASGGAGMYGSADDVLRVLETIRTG
CCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GDGFLPAALVAAMRTDHTGPAAGTRGPGWGFGYGGAVLSDPALAQSPQSAGTLQWGGVYG
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCCCCCCCEEECCCCC
HSWFVDAARGLTVLLMTNTAYEGMSGPLTLEVRDAVYGV
CHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEHHHHCCC
>Mature Secondary Structure
MNSAFDAPASAAAAAFPPADDAALAARLDRVLGAALGERRIVGAVALVARHGRLVYRRAH
CCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEEHHHH
GLAERETQRPMREDTLFRLSSITKPIVTVAVLRLVADGRMALDAPITRWLPDFAPALPDG
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCC
RAPALSVHQLLTHTAGLSYWLLEAPGSAYHTLGVSDGIDLVDFDLAENLRRIAAAPLAFE
CCCHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCCEEEHHHHHHHHHHHHCCCEEC
PGSAWRYSLALDVLGAAIERETGRALPDAVARLVTTPLGMRDTGFVAADPARFAVAYANA
CCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHEEEEECCC
APEPARITDNLDVPLPEGHGVAVRFAPSRVFDATAFASGGAGMYGSADDVLRVLETIRTG
CCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GDGFLPAALVAAMRTDHTGPAAGTRGPGWGFGYGGAVLSDPALAQSPQSAGTLQWGGVYG
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHCCCCCCCCEEECCCCC
HSWFVDAARGLTVLLMTNTAYEGMSGPLTLEVRDAVYGV
CHHHHHHCCCEEEEEEECCCCCCCCCCEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]