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The map label for this gene is sacB [H]

Identifier: 126443031

GI number: 126443031

Start: 797314

End: 798798

Strand: Reverse

Name: sacB [H]

Synonym: BURPS668_A0822

Alternate gene names: 126443031

Gene position: 798798-797314 (Counterclockwise)

Preceding gene: 126444687

Following gene: 126443805

Centisome position: 25.54

GC content: 67.41

Gene sequence:

>1485_bases
ATGTCGGTGCACGCGCAGAGCGACGGCGCCGGCCCCGCGCCGACGCCGCACACGCAGCAGGCCTACGATCCCGAAAGCCA
TTTCACGATGCGCTGGACGCGCGCGGACATGCGCCAGCTCGTCAAGCAATCGCACACGGCGGGCGCCGACAAGAACTCGC
TGCCGCCCGCGCTGACGATGCCGGACATCGCGCAGAACTTTCCGCTCGTCGATTCGAACGTGTGGGTATGGGATACCTGG
CCGCTCGCCGACATGCGCGCGAACCAGTTGAGCTACAAGGGCTGGGAAGTGATCTTCTCGCTGAGCGCCGATCCGCACGC
GGGCTACACGTTCGACGACCGCCACGTGCATGCGCGCATCGGCTTCTTCTATCGCCGCGCGGGCATTCCGGCTTCGCAGC
GTCCGGCCAACGGCGGCTGGACCTGGGGCGGCCATCTGTTTCCGGACGGCGCGAGCGCGAAGGTGTTCGGCACCGCGCCG
ATGACGAACAACGCCGAGTGGTCCGGCTCCGCGCGCCTCACGCACGGCGAGAACGTCAGCCTCTACTACACCGCGCTGTC
GTTCAATCGCTCGGCGCCCGGCGGCGCCGACATCACGCCGCCGATCGCGATCATCACGCGCGCGGACGGTCACATCCACG
CGGACGACAAGCATGTATGGTTCTCGGGATTCGACGATCACAAGGCGCTGCTGCAGCCTGAAGGCAAGATGTACCAGACG
GGCCAGCAGAACACTTACTACTCGTTCCGCGATCCGTTCGTGTTCACCGATCCCGCGCATCCGGGCAACACGTACATGGT
GTTCGAGGGCAACACGGGCGGCCCGCGCGGCGCGCGCACCTGCACCGAAGCCGATCTCGGCTACGCGCCGAACGATCCGT
ATCGCGAGGATCTGAACGCGGTGATGAACCTGGGCGCAGTGTATCAGAAGGCCAATGTCGGCCTCGCGATCGCGACGAAC
CCGCAACTGACCGAGTGGAAGTTCCTGCCGCCGCTGCTGTCGGCGAACTGTGTCGACGATCAGACCGAGCGTCCGCAGAT
CTATCTGAAGGACGGCAAGTACTACCTGTTCACGATCAGCCACCGGACGACAATGGCCGCCGGCATCGACGGCCCGGACG
GCGTGTATGGCTTCGTCGGCAACGGCATCCGCAGCGATTTCCTGCCGCTGAACGGCGGCAGCGGCCTCGTGCTCGGCAAC
CCGACCGACTTCTCCGCGCCCGCCGGCGCGCCGTACGCACAGGACCCGAACCAGAACCCGCGCGCGTTCCAGTCGTACTC
GCACTACGTGATGCCGGGCGGGCGCGTCGAGTCGTTCATCGACGCGATCGGCGCGCGGCGCGGCGGCACGCTCGCGCCGA
CGGTGAAGATCGACATCCACGGCGATTCGACGACGGTCGACCGCGCGTACGGCGCGGGCGGCCTCGGCGGCTACGGCGAC
ATTCCCGCGAACCTGCCGGCCGTGGGCGCAGGCCACCACGACTGA

Upstream 100 bases:

>100_bases
ACCGATTCACGACAATTTCATGACATATTCCAAACAAGCCGTTTCCCGTCGACACAAACGTTTGGCCCTGAGCGCGGCTG
CGCTCGCCGCAGCCGCTTGC

Downstream 100 bases:

>100_bases
CATGCCGGCGCAGGAAGGCGAACGCCGCATCCGCCTTCCTGCGCGCGGACGGCCGGACACTCCTGCGCCGTGCGCGCCGG
CTCCGGCCGCCGTCGCGTAT

Product: levansucrase

Products: NA

Alternate protein names: Beta-D-fructofuranosyl transferase; Sucrose 6-fructosyl transferase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSVHAQSDGAGPAPTPHTQQAYDPESHFTMRWTRADMRQLVKQSHTAGADKNSLPPALTMPDIAQNFPLVDSNVWVWDTW
PLADMRANQLSYKGWEVIFSLSADPHAGYTFDDRHVHARIGFFYRRAGIPASQRPANGGWTWGGHLFPDGASAKVFGTAP
MTNNAEWSGSARLTHGENVSLYYTALSFNRSAPGGADITPPIAIITRADGHIHADDKHVWFSGFDDHKALLQPEGKMYQT
GQQNTYYSFRDPFVFTDPAHPGNTYMVFEGNTGGPRGARTCTEADLGYAPNDPYREDLNAVMNLGAVYQKANVGLAIATN
PQLTEWKFLPPLLSANCVDDQTERPQIYLKDGKYYLFTISHRTTMAAGIDGPDGVYGFVGNGIRSDFLPLNGGSGLVLGN
PTDFSAPAGAPYAQDPNQNPRAFQSYSHYVMPGGRVESFIDAIGARRGGTLAPTVKIDIHGDSTTVDRAYGAGGLGGYGD
IPANLPAVGAGHHD

Sequences:

>Translated_494_residues
MSVHAQSDGAGPAPTPHTQQAYDPESHFTMRWTRADMRQLVKQSHTAGADKNSLPPALTMPDIAQNFPLVDSNVWVWDTW
PLADMRANQLSYKGWEVIFSLSADPHAGYTFDDRHVHARIGFFYRRAGIPASQRPANGGWTWGGHLFPDGASAKVFGTAP
MTNNAEWSGSARLTHGENVSLYYTALSFNRSAPGGADITPPIAIITRADGHIHADDKHVWFSGFDDHKALLQPEGKMYQT
GQQNTYYSFRDPFVFTDPAHPGNTYMVFEGNTGGPRGARTCTEADLGYAPNDPYREDLNAVMNLGAVYQKANVGLAIATN
PQLTEWKFLPPLLSANCVDDQTERPQIYLKDGKYYLFTISHRTTMAAGIDGPDGVYGFVGNGIRSDFLPLNGGSGLVLGN
PTDFSAPAGAPYAQDPNQNPRAFQSYSHYVMPGGRVESFIDAIGARRGGTLAPTVKIDIHGDSTTVDRAYGAGGLGGYGD
IPANLPAVGAGHHD
>Mature_493_residues
SVHAQSDGAGPAPTPHTQQAYDPESHFTMRWTRADMRQLVKQSHTAGADKNSLPPALTMPDIAQNFPLVDSNVWVWDTWP
LADMRANQLSYKGWEVIFSLSADPHAGYTFDDRHVHARIGFFYRRAGIPASQRPANGGWTWGGHLFPDGASAKVFGTAPM
TNNAEWSGSARLTHGENVSLYYTALSFNRSAPGGADITPPIAIITRADGHIHADDKHVWFSGFDDHKALLQPEGKMYQTG
QQNTYYSFRDPFVFTDPAHPGNTYMVFEGNTGGPRGARTCTEADLGYAPNDPYREDLNAVMNLGAVYQKANVGLAIATNP
QLTEWKFLPPLLSANCVDDQTERPQIYLKDGKYYLFTISHRTTMAAGIDGPDGVYGFVGNGIRSDFLPLNGGSGLVLGNP
TDFSAPAGAPYAQDPNQNPRAFQSYSHYVMPGGRVESFIDAIGARRGGTLAPTVKIDIHGDSTTVDRAYGAGGLGGYGDI
PANLPAVGAGHHD

Specific function: Releases fructooligosaccharides and levan, a high- molecular-mass fructosyl polymer, from sucrose. It acts more as a sucrose hydrolase than as a fructan polymerase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 68 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003469 [H]

Pfam domain/function: PF02435 Glyco_hydro_68 [H]

EC number: =2.4.1.10 [H]

Molecular weight: Translated: 53477; Mature: 53345

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHAQSDGAGPAPTPHTQQAYDPESHFTMRWTRADMRQLVKQSHTAGADKNSLPPALTM
CCEECCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCC
PDIAQNFPLVDSNVWVWDTWPLADMRANQLSYKGWEVIFSLSADPHAGYTFDDRHVHARI
CHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEE
GFFYRRAGIPASQRPANGGWTWGGHLFPDGASAKVFGTAPMTNNAEWSGSARLTHGENVS
EEEEECCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEE
LYYTALSFNRSAPGGADITPPIAIITRADGHIHADDKHVWFSGFDDHKALLQPEGKMYQT
EEEEEEEECCCCCCCCCCCCCEEEEEECCCEEECCCCEEEECCCCCCHHHCCCCCCEEEC
GQQNTYYSFRDPFVFTDPAHPGNTYMVFEGNTGGPRGARTCTEADLGYAPNDPYREDLNA
CCCCCEEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VMNLGAVYQKANVGLAIATNPQLTEWKFLPPLLSANCVDDQTERPQIYLKDGKYYLFTIS
HHHHHHHEECCCCCEEEECCCCCCEEECCCHHHCCCCCCCCCCCCEEEEECCCEEEEEEE
HRTTMAAGIDGPDGVYGFVGNGIRSDFLPLNGGSGLVLGNPTDFSAPAGAPYAQDPNQNP
CCEEEEECCCCCCCEEEECCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
RAFQSYSHYVMPGGRVESFIDAIGARRGGTLAPTVKIDIHGDSTTVDRAYGAGGLGGYGD
HHHHCCCCEECCCCHHHHHHHHHHCCCCCEECCEEEEEECCCCCCHHHHCCCCCCCCCCC
IPANLPAVGAGHHD
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SVHAQSDGAGPAPTPHTQQAYDPESHFTMRWTRADMRQLVKQSHTAGADKNSLPPALTM
CEECCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCC
PDIAQNFPLVDSNVWVWDTWPLADMRANQLSYKGWEVIFSLSADPHAGYTFDDRHVHARI
CHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEE
GFFYRRAGIPASQRPANGGWTWGGHLFPDGASAKVFGTAPMTNNAEWSGSARLTHGENVS
EEEEECCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEE
LYYTALSFNRSAPGGADITPPIAIITRADGHIHADDKHVWFSGFDDHKALLQPEGKMYQT
EEEEEEEECCCCCCCCCCCCCEEEEEECCCEEECCCCEEEECCCCCCHHHCCCCCCEEEC
GQQNTYYSFRDPFVFTDPAHPGNTYMVFEGNTGGPRGARTCTEADLGYAPNDPYREDLNA
CCCCCEEEECCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VMNLGAVYQKANVGLAIATNPQLTEWKFLPPLLSANCVDDQTERPQIYLKDGKYYLFTIS
HHHHHHHEECCCCCEEEECCCCCCEEECCCHHHCCCCCCCCCCCCEEEEECCCEEEEEEE
HRTTMAAGIDGPDGVYGFVGNGIRSDFLPLNGGSGLVLGNPTDFSAPAGAPYAQDPNQNP
CCEEEEECCCCCCCEEEECCCCCCCCCEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
RAFQSYSHYVMPGGRVESFIDAIGARRGGTLAPTVKIDIHGDSTTVDRAYGAGGLGGYGD
HHHHCCCCEECCCCHHHHHHHHHHCCCCCEECCEEEEEECCCCCCHHHHCCCCCCCCCCC
IPANLPAVGAGHHD
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8704949; 10214721 [H]