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The map label for this gene is dut

Identifier: 126209422

GI number: 126209422

Start: 2196192

End: 2196647

Strand: Reverse

Name: dut

Synonym: APL_1968

Alternate gene names: 126209422

Gene position: 2196647-2196192 (Counterclockwise)

Preceding gene: 126209423

Following gene: 126209421

Centisome position: 96.58

GC content: 46.71

Gene sequence:

>456_bases
ATGAAACAAATCGACTTAAAAATTTTAGACGGCCGTATCGGCAACGAATTTCCATTACCGGCTTATGCAACCGAAGGTTC
GGCAGGCTTGGATTTACGAGCCTTAACTGAAAGCGCTTTAACCGTAGCACCGGGTCAAACCGTTTTGATCCCAACCGGTA
TTTCCATTTATATCGCCGATCCGAATTTAGCCGCAGTGATTTTACCTCGTTCCGGTTTAGGGCATAAAAACGGGATCGTG
TTAGGTAATTTGGTCGGTTTGATCGATAGCGATTATCAGGGCCCGTTGATGGTTTCGTTATGGAACCGCAGCGATAAACC
GTTTACGGTGGAAGTCGGCGACCGCATTGCTCAGTTAGTTTTCGTGCCGGTAGTGCAAGCCAGATTCAATATCGTAAACG
ATTTTGCACAAACCGAACGCGGTGAAGGCGGTTTCGGTCATTCAGGTAAACAGTAA

Upstream 100 bases:

>100_bases
ACGGTGAGAAAATGCTACCGCTTGCCGATAAAAGCAAACTGGCGGAAGCCTTAGTTCACGAGATTATTGAGCAATTTAAA
CACGACAAATAGGAACATAA

Downstream 100 bases:

>100_bases
TATTCGCTACTCAAGCAAGCGGTCGAATTTGTAAAAAATTTTGCAAAAATGACCGCTTGCGACTTCTTTTGATAATAAGC
AGATTGGCAAAACAAAGAGA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKQIDLKILDGRIGNEFPLPAYATEGSAGLDLRALTESALTVAPGQTVLIPTGISIYIADPNLAAVILPRSGLGHKNGIV
LGNLVGLIDSDYQGPLMVSLWNRSDKPFTVEVGDRIAQLVFVPVVQARFNIVNDFAQTERGEGGFGHSGKQ

Sequences:

>Translated_151_residues
MKQIDLKILDGRIGNEFPLPAYATEGSAGLDLRALTESALTVAPGQTVLIPTGISIYIADPNLAAVILPRSGLGHKNGIV
LGNLVGLIDSDYQGPLMVSLWNRSDKPFTVEVGDRIAQLVFVPVVQARFNIVNDFAQTERGEGGFGHSGKQ
>Mature_151_residues
MKQIDLKILDGRIGNEFPLPAYATEGSAGLDLRALTESALTVAPGQTVLIPTGISIYIADPNLAAVILPRSGLGHKNGIV
LGNLVGLIDSDYQGPLMVSLWNRSDKPFTVEVGDRIAQLVFVPVVQARFNIVNDFAQTERGEGGFGHSGKQ

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=33.1034482758621, Blast_Score=74, Evalue=6e-14,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=33.1034482758621, Blast_Score=74, Evalue=6e-14,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=33.1034482758621, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=70.8609271523179, Blast_Score=231, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71988561, Length=131, Percent_Identity=37.4045801526718, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319729, Length=132, Percent_Identity=34.8484848484849, Blast_Score=73, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583610, Length=131, Percent_Identity=36.6412213740458, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=36.6412213740458, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_ACTP2 (A3N3Q6)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054647.1
- ProteinModelPortal:   A3N3Q6
- SMR:   A3N3Q6
- STRING:   A3N3Q6
- GeneID:   4849970
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1968
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   A3N3Q6
- ProtClustDB:   PRK00601
- BioCyc:   APLE416269:APL_1968-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16083; Mature: 16083

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIDLKILDGRIGNEFPLPAYATEGSAGLDLRALTESALTVAPGQTVLIPTGISIYIAD
CCEEEEEEECCCCCCCCCCCCEECCCCCCCCCEEECCCCEEECCCCEEEEECCCEEEEEC
PNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGPLMVSLWNRSDKPFTVEVGDRIAQLV
CCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHHH
FVPVVQARFNIVNDFAQTERGEGGFGHSGKQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQIDLKILDGRIGNEFPLPAYATEGSAGLDLRALTESALTVAPGQTVLIPTGISIYIAD
CCEEEEEEECCCCCCCCCCCCEECCCCCCCCCEEECCCCEEECCCCEEEEECCCEEEEEC
PNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGPLMVSLWNRSDKPFTVEVGDRIAQLV
CCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHHH
FVPVVQARFNIVNDFAQTERGEGGFGHSGKQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA