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The map label for this gene is miaA [H]

Identifier: 126209414

GI number: 126209414

Start: 2190802

End: 2191716

Strand: Direct

Name: miaA [H]

Synonym: APL_1960

Alternate gene names: 126209414

Gene position: 2190802-2191716 (Clockwise)

Preceding gene: 126209413

Following gene: 126209415

Centisome position: 96.32

GC content: 45.9

Gene sequence:

>915_bases
ATGGGTCCAACGGCTTCGGGTAAAACCGATTTGGCGATTGCTTTGCGCCAAACGCTTCCGGTGGAAGTAATTAGCGTAGA
TTCCGCACTGATTTATAAGGGGATGGATATCGGTACGGCAAAACCGAGTAAAGCGGAATTGGAGCTTGCCCCTCACCGCT
TGATTGATATTTTGGATCCGAGCGAAAGTTATTCGGCGATGAATTTCCGAGAAGACGCTTTGCGTGAAATGGCGGAAATT
ACGGCAAGCGGACGAATCCCGTTATTGGCCGGCGGCACAATGTTATATTATAAAGCCTTATTGGAAGGGCTTTCGCCGTT
ACCGTCTGCCGATCCGGAGATTCGTGCCGAAATAGAAGCGAAAGCCGAGCAAATCGGTTGGAGCGGTTTGCATCGCGAAT
TATTGGCGATTGACCCTATTGCCGGAAAGCGCATTAATCCGAATGACAGCCAACGAATTAATCGTGCTTTGGAAGTGTTC
TATATTACCGGTAAAACAATGACGGAGCTGACCGCCCAACAGGGCGATAGCTTGCCTTATAACGTCCTTCAATTTGCTAT
TGCACCGCAAGACAGAGCGGTATTACATCAACGTATCGAACAACGTTTTTATAAAATGATGGAACTCGGCTTTCAACAAG
AAGTCGAAAAATTACGTGCCAGAAGCGATTTACACAAAGATCTTCCTTCGATTCGTTGCGTCGGTTATCGTCAAATGTGG
GAATATCTTGACGGTGATATTTCACTGGATGAAGCGATTTACAAAGGAATCTGTGCGACTCGCCAGCTTGCCAAGCGTCA
AATTACTTGGCTGCGAGGTTGGAACAGTGAAATAACGTGGCTGGACAGCTTAGATCCGACCAGTTCTAAGCTAAAAATGA
TCGAAAAGATCGCACAAAATTCAATTAAATTATGA

Upstream 100 bases:

>100_bases
ATTATGCCGATTTGGGCTTTTGGCAAATTTCCGTTAAAATTCGACTGCTTGTAACTAAACAGAAAATAAAATGAATAAGA
AACCTTTAGCGATTTTTTTA

Downstream 100 bases:

>100_bases
TAATATCATTTTTGCCTTTGGCACTAAGACGATATTTACCACAACAATAAAAAAGGAAAAGAAAATGGCAAAAGGTCAAT
CTTTACAAGATCCATATTTG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGPTASGKTDLAIALRQTLPVEVISVDSALIYKGMDIGTAKPSKAELELAPHRLIDILDPSESYSAMNFREDALREMAEI
TASGRIPLLAGGTMLYYKALLEGLSPLPSADPEIRAEIEAKAEQIGWSGLHRELLAIDPIAGKRINPNDSQRINRALEVF
YITGKTMTELTAQQGDSLPYNVLQFAIAPQDRAVLHQRIEQRFYKMMELGFQQEVEKLRARSDLHKDLPSIRCVGYRQMW
EYLDGDISLDEAIYKGICATRQLAKRQITWLRGWNSEITWLDSLDPTSSKLKMIEKIAQNSIKL

Sequences:

>Translated_304_residues
MGPTASGKTDLAIALRQTLPVEVISVDSALIYKGMDIGTAKPSKAELELAPHRLIDILDPSESYSAMNFREDALREMAEI
TASGRIPLLAGGTMLYYKALLEGLSPLPSADPEIRAEIEAKAEQIGWSGLHRELLAIDPIAGKRINPNDSQRINRALEVF
YITGKTMTELTAQQGDSLPYNVLQFAIAPQDRAVLHQRIEQRFYKMMELGFQQEVEKLRARSDLHKDLPSIRCVGYRQMW
EYLDGDISLDEAIYKGICATRQLAKRQITWLRGWNSEITWLDSLDPTSSKLKMIEKIAQNSIKL
>Mature_303_residues
GPTASGKTDLAIALRQTLPVEVISVDSALIYKGMDIGTAKPSKAELELAPHRLIDILDPSESYSAMNFREDALREMAEIT
ASGRIPLLAGGTMLYYKALLEGLSPLPSADPEIRAEIEAKAEQIGWSGLHRELLAIDPIAGKRINPNDSQRINRALEVFY
ITGKTMTELTAQQGDSLPYNVLQFAIAPQDRAVLHQRIEQRFYKMMELGFQQEVEKLRARSDLHKDLPSIRCVGYRQMWE
YLDGDISLDEAIYKGICATRQLAKRQITWLRGWNSEITWLDSLDPTSSKLKMIEKIAQNSIKL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=305, Percent_Identity=29.5081967213115, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1790613, Length=298, Percent_Identity=68.4563758389262, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25144712, Length=294, Percent_Identity=30.2721088435374, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=283, Percent_Identity=28.9752650176678, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24649830, Length=266, Percent_Identity=31.203007518797, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 34160; Mature: 34029

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTASGKTDLAIALRQTLPVEVISVDSALIYKGMDIGTAKPSKAELELAPHRLIDILDP
CCCCCCCCCHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCCCCEEECHHHHHCCCCC
SESYSAMNFREDALREMAEITASGRIPLLAGGTMLYYKALLEGLSPLPSADPEIRAEIEA
CCCCHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KAEQIGWSGLHRELLAIDPIAGKRINPNDSQRINRALEVFYITGKTMTELTAQQGDSLPY
HHHHHCHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCH
NVLQFAIAPQDRAVLHQRIEQRFYKMMELGFQQEVEKLRARSDLHKDLPSIRCVGYRQMW
HHHHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
EYLDGDISLDEAIYKGICATRQLAKRQITWLRGWNSEITWLDSLDPTSSKLKMIEKIAQN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHH
SIKL
CCCC
>Mature Secondary Structure 
GPTASGKTDLAIALRQTLPVEVISVDSALIYKGMDIGTAKPSKAELELAPHRLIDILDP
CCCCCCCCHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCCCCEEECHHHHHCCCCC
SESYSAMNFREDALREMAEITASGRIPLLAGGTMLYYKALLEGLSPLPSADPEIRAEIEA
CCCCHHCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KAEQIGWSGLHRELLAIDPIAGKRINPNDSQRINRALEVFYITGKTMTELTAQQGDSLPY
HHHHHCHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCH
NVLQFAIAPQDRAVLHQRIEQRFYKMMELGFQQEVEKLRARSDLHKDLPSIRCVGYRQMW
HHHHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
EYLDGDISLDEAIYKGICATRQLAKRQITWLRGWNSEITWLDSLDPTSSKLKMIEKIAQN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHH
SIKL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA