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The map label for this gene is nudF [H]

Identifier: 126209401

GI number: 126209401

Start: 2175500

End: 2176123

Strand: Reverse

Name: nudF [H]

Synonym: APL_1943

Alternate gene names: 126209401

Gene position: 2176123-2175500 (Counterclockwise)

Preceding gene: 126209402

Following gene: 126209400

Centisome position: 95.68

GC content: 45.03

Gene sequence:

>624_bases
ATGACCAAACTTCGTCAATTTTCACAACAGGATCTTCATCTGATTAAAGAAGAAACCGTTTATAAAGGACATTTTGAATT
AAAGAAAATGTATTTCCGACATAAGCTATTCTCCGGTGAAATGAGCGGTGAAATCGTGCGTGAATTACTGATTAAAGGGG
CGGCGGCGGCATTAATCGCTTACGATCCCGTTCGTGATAGCGTTGTTTTAGTCGAACAAGTTCGTATCGGCGCCTATGAT
CCGAAATCGGATGCTTCTCCTTGGTTACTCGAACTGGTTGCCGGTATGGTGGATAAAGGTAATGAAGATCCTGCCGAAGT
GGCGATTCGAGAAGCGCAAGAAGAAGCGGGATTAGTCGTCAATCAGGTCGAGCATGCGTTAAGTATTTGGGACAGCCCGG
GCGGACAATTGGAACGCCTACATTTGTTCCTCGGCTTAATCGACAGTTCAACCGTTCCATCCGGTGCAACGCACGGCTTA
GAAGAGGAAGGCGAAGATATTCTGGTACACGTCGTCAGCCGAGAACAAGCCTATCAATGGGTGTGTGAAGGCAAAATCGA
TAATGTTATCGCAGTGGTCGGTTTACAATGGTTACAACTTAATTATCAAAAATATCGAGTGTAA

Upstream 100 bases:

>100_bases
TGCAATGCACACACTATTGTTCGCATTATCTATTTTAGTGATGATCATCGCTCTCTATCTTGTTCATTCAGACCGCTTAC
GTAAGAGCTAAGGAAATAAT

Downstream 100 bases:

>100_bases
TGAGCGTTTTCCGATAACGACGAATACTTTTGCCCTCTTTAATGAGGGCAAATTTTTTGAAGTATCCGACAAAAAAACGT
AAATTTCTGTGAAAAATGAG

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTKLRQFSQQDLHLIKEETVYKGHFELKKMYFRHKLFSGEMSGEIVRELLIKGAAAALIAYDPVRDSVVLVEQVRIGAYD
PKSDASPWLLELVAGMVDKGNEDPAEVAIREAQEEAGLVVNQVEHALSIWDSPGGQLERLHLFLGLIDSSTVPSGATHGL
EEEGEDILVHVVSREQAYQWVCEGKIDNVIAVVGLQWLQLNYQKYRV

Sequences:

>Translated_207_residues
MTKLRQFSQQDLHLIKEETVYKGHFELKKMYFRHKLFSGEMSGEIVRELLIKGAAAALIAYDPVRDSVVLVEQVRIGAYD
PKSDASPWLLELVAGMVDKGNEDPAEVAIREAQEEAGLVVNQVEHALSIWDSPGGQLERLHLFLGLIDSSTVPSGATHGL
EEEGEDILVHVVSREQAYQWVCEGKIDNVIAVVGLQWLQLNYQKYRV
>Mature_206_residues
TKLRQFSQQDLHLIKEETVYKGHFELKKMYFRHKLFSGEMSGEIVRELLIKGAAAALIAYDPVRDSVVLVEQVRIGAYDP
KSDASPWLLELVAGMVDKGNEDPAEVAIREAQEEAGLVVNQVEHALSIWDSPGGQLERLHLFLGLIDSSTVPSGATHGLE
EEGEDILVHVVSREQAYQWVCEGKIDNVIAVVGLQWLQLNYQKYRV

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=200, Percent_Identity=53, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1788810, Length=194, Percent_Identity=30.9278350515464, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23240; Mature: 23109

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLRQFSQQDLHLIKEETVYKGHFELKKMYFRHKLFSGEMSGEIVRELLIKGAAAALIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEE
YDPVRDSVVLVEQVRIGAYDPKSDASPWLLELVAGMVDKGNEDPAEVAIREAQEEAGLVV
ECCCCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHH
NQVEHALSIWDSPGGQLERLHLFLGLIDSSTVPSGATHGLEEEGEDILVHVVSREQAYQW
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
VCEGKIDNVIAVVGLQWLQLNYQKYRV
HHCCCHHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
TKLRQFSQQDLHLIKEETVYKGHFELKKMYFRHKLFSGEMSGEIVRELLIKGAAAALIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEE
YDPVRDSVVLVEQVRIGAYDPKSDASPWLLELVAGMVDKGNEDPAEVAIREAQEEAGLVV
ECCCCCCEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHH
NQVEHALSIWDSPGGQLERLHLFLGLIDSSTVPSGATHGLEEEGEDILVHVVSREQAYQW
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
VCEGKIDNVIAVVGLQWLQLNYQKYRV
HHCCCHHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10542272 [H]