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The map label for this gene is tpiA [H]

Identifier: 126209385

GI number: 126209385

Start: 2161944

End: 2162714

Strand: Reverse

Name: tpiA [H]

Synonym: APL_1925

Alternate gene names: 126209385

Gene position: 2162714-2161944 (Counterclockwise)

Preceding gene: 126209392

Following gene: 126209377

Centisome position: 95.09

GC content: 48.51

Gene sequence:

>771_bases
ATGGCACGTCGTCCACTTGTAATGGGTAACTGGAAATTAAACGGTAGCAAAGCATTTACAAAAGAGTTAATCGCCGGCTT
AAAAGCGGAATTAGCGGATGTTAAAGGCTGTGACGTTGCAATCGCGCCGCCGGTAATGTACTTAGCGGAAGCGGAAGCGG
CATTAGCGGGTCAATCAGTCATCGCATTAGGCGCACAAAACGTAGACGTAAACGTACAAGGTGCATTTACCGGTGATATT
TCAACCGAAATGTTAAAAGACTTCGGTGCAAAATATATTATTATCGGTCACTCCGAGCGTCGTACTTATCACAAAGAATG
CGATACGTTCATTGCGAAAAAATTCGCTGCGTTAAAAGCGGCGGGTTTAGTACCGGTATTATGTATCGGTGAAACCGAAG
CGGAAAACGAAGCGGGTCAAACCGAAGCGGTATGCGCAAAACAAATTGATGCGGTAATCGATGCGTTAGGCGTTGAAGCG
TTTAACGGTGCGGTAATCGCCTACGAACCGATTTGGGCTATCGGTACCGGTAAATCCGCAACTCCTGCGCAAGCGCAAGC
GGTTCACGCATTCATCCGCGGTCACATTGCAGCGAAATCACAAGCGGTTGCGGATCAAGTGATTATCCAATACGGCGGTT
CGGTAAACGATGCGAATGCGGCGGAATTATTCACTCAGCCGGATATCGACGGTGCGTTAGTCGGCGGTGCGTCATTAAAA
GCGCCTGCATTTGCGGTTATCGTCAAAGCGGCGGAAAAAGCGAAAGCGTAA

Upstream 100 bases:

>100_bases
ATAGGTCGCAATTCTTTGCGAAAAGAATCAAAAATTTTCATAAAATTTGGTATAGTTCGTATTACTTATTTTTATATTCA
AACTCAATAGGAGAAATCAC

Downstream 100 bases:

>100_bases
TTTGTGATATAATCAGTCATCACTAATAATAAACAAGCGGTTGAATTTACAAGATTTTTTGCAAATTCAACCGCTTTTCT
ATTTGATCGGTTCGTATGTC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MARRPLVMGNWKLNGSKAFTKELIAGLKAELADVKGCDVAIAPPVMYLAEAEAALAGQSVIALGAQNVDVNVQGAFTGDI
STEMLKDFGAKYIIIGHSERRTYHKECDTFIAKKFAALKAAGLVPVLCIGETEAENEAGQTEAVCAKQIDAVIDALGVEA
FNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVADQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLK
APAFAVIVKAAEKAKA

Sequences:

>Translated_256_residues
MARRPLVMGNWKLNGSKAFTKELIAGLKAELADVKGCDVAIAPPVMYLAEAEAALAGQSVIALGAQNVDVNVQGAFTGDI
STEMLKDFGAKYIIIGHSERRTYHKECDTFIAKKFAALKAAGLVPVLCIGETEAENEAGQTEAVCAKQIDAVIDALGVEA
FNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVADQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLK
APAFAVIVKAAEKAKA
>Mature_255_residues
ARRPLVMGNWKLNGSKAFTKELIAGLKAELADVKGCDVAIAPPVMYLAEAEAALAGQSVIALGAQNVDVNVQGAFTGDIS
TEMLKDFGAKYIIIGHSERRTYHKECDTFIAKKFAALKAAGLVPVLCIGETEAENEAGQTEAVCAKQIDAVIDALGVEAF
NGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVADQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLKA
PAFAVIVKAAEKAKA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=252, Percent_Identity=46.8253968253968, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI4507645, Length=252, Percent_Identity=46.8253968253968, Blast_Score=194, Evalue=6e-50,
Organism=Escherichia coli, GI1790353, Length=253, Percent_Identity=73.5177865612648, Blast_Score=369, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=49.402390438247, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=44.6215139442231, Blast_Score=196, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28572008, Length=253, Percent_Identity=46.6403162055336, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28572006, Length=253, Percent_Identity=46.6403162055336, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28572004, Length=253, Percent_Identity=46.6403162055336, Blast_Score=198, Evalue=3e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26523; Mature: 26391

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPLVMGNWKLNGSKAFTKELIAGLKAELADVKGCDVAIAPPVMYLAEAEAALAGQSV
CCCCCEEEECEEECCCHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCEE
IALGAQNVDVNVQGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKECDTFIAKKFAALKA
EEECCCCCEEEEEEEEECCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AGLVPVLCIGETEAENEAGQTEAVCAKQIDAVIDALGVEAFNGAVIAYEPIWAIGTGKSA
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCEEEEECCCCC
TPAQAQAVHAFIRGHIAAKSQAVADQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCC
APAFAVIVKAAEKAKA
CCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARRPLVMGNWKLNGSKAFTKELIAGLKAELADVKGCDVAIAPPVMYLAEAEAALAGQSV
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCEE
IALGAQNVDVNVQGAFTGDISTEMLKDFGAKYIIIGHSERRTYHKECDTFIAKKFAALKA
EEECCCCCEEEEEEEEECCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
AGLVPVLCIGETEAENEAGQTEAVCAKQIDAVIDALGVEAFNGAVIAYEPIWAIGTGKSA
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCEEEEECCCCC
TPAQAQAVHAFIRGHIAAKSQAVADQVIIQYGGSVNDANAAELFTQPDIDGALVGGASLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCC
APAFAVIVKAAEKAKA
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA