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The map label for this gene is metJ

Identifier: 126209377

GI number: 126209377

Start: 2151070

End: 2151387

Strand: Reverse

Name: metJ

Synonym: APL_1917

Alternate gene names: 126209377

Gene position: 2151387-2151070 (Counterclockwise)

Preceding gene: 126209385

Following gene: 126209376

Centisome position: 94.59

GC content: 44.97

Gene sequence:

>318_bases
ATGGCAGATTGGAGTGGGGAATACATTAGCCCGTATGCCGAACACGGCAAAAAAAGTGAACAAGTGAAAAAGATCACGGT
ATCGATCCCGATTAAAGTATTAGAAATTTTAACCAACGAACGTACTCGACGTCAGATCAAAAACTTGCGTCATGCAACCA
ATAGCGAATTACTATGCGAAGCCTTTTTGCATGCCTTTACCGGACAGCCGTTGCCGACGGATGACGATCTGATGAAAGAA
CGCAGCGATGAGATTCCGGAAGAGGCTAAAGCGAAAATGCGCGAATTGGGTATCGACCCTGATAACTGGCAATATTGA

Upstream 100 bases:

>100_bases
CTAAGTAAAATTGAAGATAGAAATCTAGACGTCCAGATTGCGGGAGAATGTTGTTTTGATTACAATACCTGCAATTTTTA
TATAAAGCAGGATTTTAACT

Downstream 100 bases:

>100_bases
TAATTAACGAGCCTCATTTTTGAGGCTTTCTTACAATGGAAAAGGAGTCTTATGAATTATCGACAACTCGCGAAAGAAGC
TCGCTGGGCGCTCTGGCTTG

Product: transcriptional repressor protein MetJ

Products: NA

Alternate protein names: Met regulon regulatory protein metJ

Number of amino acids: Translated: 105; Mature: 104

Protein sequence:

>105_residues
MADWSGEYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQIKNLRHATNSELLCEAFLHAFTGQPLPTDDDLMKE
RSDEIPEEAKAKMRELGIDPDNWQY

Sequences:

>Translated_105_residues
MADWSGEYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQIKNLRHATNSELLCEAFLHAFTGQPLPTDDDLMKE
RSDEIPEEAKAKMRELGIDPDNWQY
>Mature_104_residues
ADWSGEYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQIKNLRHATNSELLCEAFLHAFTGQPLPTDDDLMKER
SDEIPEEAKAKMRELGIDPDNWQY

Specific function: This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis

COG id: COG3060

COG function: function code KE; Transcriptional regulator of met regulon

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metJ family

Homologues:

Organism=Escherichia coli, GI1790373, Length=105, Percent_Identity=83.8095238095238, Blast_Score=189, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METJ_ACTP2 (A3N3L1)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054602.1
- ProteinModelPortal:   A3N3L1
- SMR:   A3N3L1
- STRING:   A3N3L1
- GeneID:   4848627
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1917
- eggNOG:   COG3060
- HOGENOM:   HBG297188
- OMA:   DLRKERN
- PhylomeDB:   A3N3L1
- ProtClustDB:   PRK05264
- BioCyc:   APLE416269:APL_1917-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00744
- InterPro:   IPR002084
- InterPro:   IPR010985
- Gene3D:   G3DSA:1.10.140.10
- ProDom:   PD020365

Pfam domain/function: PF01340 MetJ; SSF47598 Met_repress_like

EC number: NA

Molecular weight: Translated: 12156; Mature: 12025

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWSGEYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQIKNLRHATNSELLCE
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAFTGQPLPTDDDLMKERSDEIPEEAKAKMRELGIDPDNWQY
HHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADWSGEYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQIKNLRHATNSELLCE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAFTGQPLPTDDDLMKERSDEIPEEAKAKMRELGIDPDNWQY
HHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA