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The map label for this gene is mscS [H]

Identifier: 126209340

GI number: 126209340

Start: 2109487

End: 2110398

Strand: Direct

Name: mscS [H]

Synonym: APL_1880

Alternate gene names: 126209340

Gene position: 2109487-2110398 (Clockwise)

Preceding gene: 126209337

Following gene: 126209341

Centisome position: 92.75

GC content: 39.69

Gene sequence:

>912_bases
ATGTCAGCAGAAAAAGCTACTGAAACTACACCAAATATCAATGTTGTTGCGGAAACGGAAAAAGCGATTAATAAAGTCAG
CAATATGGATTTCAACACTATGCTCAATGAGTGGATTATCCCTTACGGTACAAAAATTCTGCTTGCGATTGCGATTTTTG
TGATTGGTAAGTTTCTTGCCCGAGTAATTAGCACCTTATTGGGTAAAGCAGCATTGAAATCAACCAAAGATGAAATGCTA
CAAAGTTTTATTTCCTCTATCAGCTACTTCTTATTGCTCTTAATTGTCGTCATTGCTGCCCTTTCACAACTCGGCATTAA
TACCAGCTCATTGGTTGCCTTAATCGGTGCGGCAGGTTTAGCCATTGGTCTATCACTGCAAAACTCATTACAAAACTTTG
CGGCAGGCGTGATGTTATTAATCTTCAAACCGTTCCGTAAAGGCGATTTAATCGAAACCGGCGGTATGACCGGTACGGTT
GAGCAAATGGGCTTATTAGTATTGGAACTGCGTACCGGCGATAACAAAACCGTATTGATTCCAAACGGCAAAGTGTTCTC
AGACAGCATCGTTAACTACTCTGCAAACGATGTTCGCCGTATTGATTTTACTTTTGATATTTCGTACGAATCTAACTTAA
AAGAGGCGAAAGAAATTGTATCTCACCTCTTAGAAAATAACGCTTTAGTATTAAAAGATCCGGCATTTGTCGTGGCAGTC
GGTGCATTATCAGCACATAGCGTACAACTTGTGGTTCGTCATTGGGTGAAAACAGCTGATTACTGGACGGCATACTGGGG
AATTACCGAAATGGTCAAACTTGAATTTGATAAAGCCGGTATTGAGATTCCATACAATCAAATGAATCTTCACCTTGCAG
ACAGTAAAAGCATTGCGATTGAAAATAAATAA

Upstream 100 bases:

>100_bases
ATAATTCCATTGTATTAATTCATATTTAATACAATGGAATTATTTCTTTCCAGCCTCAGCCATTACGTTGAGAAAATATC
AACTTTTTAAGGAAAATAAA

Downstream 100 bases:

>100_bases
TTATTTGAGACATAAATAACAGGCGGTAGGTTTTTTGGTAAATTTTACCAAGAATCCGACCGCTTTTTTGTTGTCCGGAA
AATGCATAAAATAGTATCAA

Product: small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSAEKATETTPNINVVAETEKAINKVSNMDFNTMLNEWIIPYGTKILLAIAIFVIGKFLARVISTLLGKAALKSTKDEML
QSFISSISYFLLLLIVVIAALSQLGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGTV
EQMGLLVLELRTGDNKTVLIPNGKVFSDSIVNYSANDVRRIDFTFDISYESNLKEAKEIVSHLLENNALVLKDPAFVVAV
GALSAHSVQLVVRHWVKTADYWTAYWGITEMVKLEFDKAGIEIPYNQMNLHLADSKSIAIENK

Sequences:

>Translated_303_residues
MSAEKATETTPNINVVAETEKAINKVSNMDFNTMLNEWIIPYGTKILLAIAIFVIGKFLARVISTLLGKAALKSTKDEML
QSFISSISYFLLLLIVVIAALSQLGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGTV
EQMGLLVLELRTGDNKTVLIPNGKVFSDSIVNYSANDVRRIDFTFDISYESNLKEAKEIVSHLLENNALVLKDPAFVVAV
GALSAHSVQLVVRHWVKTADYWTAYWGITEMVKLEFDKAGIEIPYNQMNLHLADSKSIAIENK
>Mature_302_residues
SAEKATETTPNINVVAETEKAINKVSNMDFNTMLNEWIIPYGTKILLAIAIFVIGKFLARVISTLLGKAALKSTKDEMLQ
SFISSISYFLLLLIVVIAALSQLGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGTVE
QMGLLVLELRTGDNKTVLIPNGKVFSDSIVNYSANDVRRIDFTFDISYESNLKEAKEIVSHLLENNALVLKDPAFVVAVG
ALSAHSVQLVVRHWVKTADYWTAYWGITEMVKLEFDKAGIEIPYNQMNLHLADSKSIAIENK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=258, Percent_Identity=37.2093023255814, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI2367355, Length=192, Percent_Identity=30.2083333333333, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786670, Length=221, Percent_Identity=24.4343891402715, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787591, Length=194, Percent_Identity=27.319587628866, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 33130; Mature: 32999

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEKATETTPNINVVAETEKAINKVSNMDFNTMLNEWIIPYGTKILLAIAIFVIGKFLA
CCCCCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RVISTLLGKAALKSTKDEMLQSFISSISYFLLLLIVVIAALSQLGINTSSLVALIGAAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGTVEQMGLLVLELRTGDNKTVLI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHCEEEEEEECCCCCEEEE
PNGKVFSDSIVNYSANDVRRIDFTFDISYESNLKEAKEIVSHLLENNALVLKDPAFVVAV
ECCCEECCHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEE
GALSAHSVQLVVRHWVKTADYWTAYWGITEMVKLEFDKAGIEIPYNQMNLHLADSKSIAI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCEEE
ENK
CCC
>Mature Secondary Structure 
SAEKATETTPNINVVAETEKAINKVSNMDFNTMLNEWIIPYGTKILLAIAIFVIGKFLA
CCCCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RVISTLLGKAALKSTKDEMLQSFISSISYFLLLLIVVIAALSQLGINTSSLVALIGAAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGTVEQMGLLVLELRTGDNKTVLI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHCEEEEEEECCCCCEEEE
PNGKVFSDSIVNYSANDVRRIDFTFDISYESNLKEAKEIVSHLLENNALVLKDPAFVVAV
ECCCEECCHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEE
GALSAHSVQLVVRHWVKTADYWTAYWGITEMVKLEFDKAGIEIPYNQMNLHLADSKSIAI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCEEE
ENK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]