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The map label for this gene is dapE

Identifier: 126209335

GI number: 126209335

Start: 2102272

End: 2103405

Strand: Reverse

Name: dapE

Synonym: APL_1873

Alternate gene names: 126209335

Gene position: 2103405-2102272 (Counterclockwise)

Preceding gene: 126209336

Following gene: 126209334

Centisome position: 92.48

GC content: 44.53

Gene sequence:

>1134_bases
ATGAAACACAACATCATCAATCTTGCGCAGGATTTAATTCGCCGTCCGTCCGTTAGCCCGGATGATCAGGGCTGCCAACA
AGTGATTGCGGAACGCTTGGCGCAATTAGGCTTTACACTCGAATGGTTGCCTTTCGGCGATACGCTAAATTTATGGGCAA
CTCACGGTACGCAAGATCCTTGTGTGGTATTTGCCGGACATACCGATGTCGTACCGGTTGGCGATGAAACACAGTGGCAA
TATCCACCGTTTTCCGCAGAAATTGTGGACGGTACACTTTATGGGCGTGGTGCGGCGGATATGAAAGGTTCTCTTGCCGC
ATTAGTGATTGCTGCCGAAACTTTTGTCAAAAATAATCCGAATCATAAAGGTAAAATTGCCCTATTGATCACTTCTGATG
AAGAAGCAGCTGCCAAAGCCGGTACGGTAAAAGTCGTTGAAACACTAATGGCTCGTCAAGAAGCAGTCCATTATGCCGTT
GTAGGCGAACCTTCTAGCGGCAAAGTGTTAGGCGATGTGATTAAAAACGGCAGACGAGGCTCAATTACCGGTGAACTTTA
TATTGAAGGTGTGCAAGGTCATGTCGCTTATCCGCATTTAGCTGAAAATCCGGTACATACTTCATTGAATTTCTTAACCG
AATTAACTACTTATCAATGGGATAACGGCAATGAATTTTTCCCACCGACCAGTTTGCAAATCGCTAATATTAAAGCGGGA
ACAGGCAGTAATAATGTTATTCCGGGCGAATTATACGTGCAATTTAACTTACGCTATTGCACCGAAGTGACCGATGAAAT
TATCAAAAACAAAGTGGCGGAAATGTTAGCTAAACATCAGCTAAAACACCGTATTAGTTGGAATTTATCCGGTCAGCCGT
TTTTAGCCGGTAATGGAGAATTGGTTAAAGCGACGGTACAGGCGGTCGAAAACGTCACAAAAATTACCCCTCGTTTAGAT
ACGAGCGGAGGGACTTCAGACGGTCGCTTTATCGCATTAATGGGTGCGGAAGTGGTCGAGTTCGGGCCGTTAAACGCGAC
TATTCATAAAGTGAATGAATGTGTCAGCGTAGAAGACCTTGGTAAATGCGGAGAAGTGTACTATCACATCCTCGAACGTT
TACTTAAAAGTTAA

Upstream 100 bases:

>100_bases
CGTCCTATTATTTTACAAGACGGTATTGCGCTAATCGGCTTTGATGCCAAACAATACGAACAAGCATTCGGTAAATAATC
TGATTTTGGAAAATAAATAT

Downstream 100 bases:

>100_bases
TACCGAGGAAATATTAAAATGATCGCAAACCTCGAACAAATTCTCACCGGCAAAACACGTGAACACCTTGTGCCGTTGCC
GAATCCGTTATCGGATAAAC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKHNIINLAQDLIRRPSVSPDDQGCQQVIAERLAQLGFTLEWLPFGDTLNLWATHGTQDPCVVFAGHTDVVPVGDETQWQ
YPPFSAEIVDGTLYGRGAADMKGSLAALVIAAETFVKNNPNHKGKIALLITSDEEAAAKAGTVKVVETLMARQEAVHYAV
VGEPSSGKVLGDVIKNGRRGSITGELYIEGVQGHVAYPHLAENPVHTSLNFLTELTTYQWDNGNEFFPPTSLQIANIKAG
TGSNNVIPGELYVQFNLRYCTEVTDEIIKNKVAEMLAKHQLKHRISWNLSGQPFLAGNGELVKATVQAVENVTKITPRLD
TSGGTSDGRFIALMGAEVVEFGPLNATIHKVNECVSVEDLGKCGEVYYHILERLLKS

Sequences:

>Translated_377_residues
MKHNIINLAQDLIRRPSVSPDDQGCQQVIAERLAQLGFTLEWLPFGDTLNLWATHGTQDPCVVFAGHTDVVPVGDETQWQ
YPPFSAEIVDGTLYGRGAADMKGSLAALVIAAETFVKNNPNHKGKIALLITSDEEAAAKAGTVKVVETLMARQEAVHYAV
VGEPSSGKVLGDVIKNGRRGSITGELYIEGVQGHVAYPHLAENPVHTSLNFLTELTTYQWDNGNEFFPPTSLQIANIKAG
TGSNNVIPGELYVQFNLRYCTEVTDEIIKNKVAEMLAKHQLKHRISWNLSGQPFLAGNGELVKATVQAVENVTKITPRLD
TSGGTSDGRFIALMGAEVVEFGPLNATIHKVNECVSVEDLGKCGEVYYHILERLLKS
>Mature_377_residues
MKHNIINLAQDLIRRPSVSPDDQGCQQVIAERLAQLGFTLEWLPFGDTLNLWATHGTQDPCVVFAGHTDVVPVGDETQWQ
YPPFSAEIVDGTLYGRGAADMKGSLAALVIAAETFVKNNPNHKGKIALLITSDEEAAAKAGTVKVVETLMARQEAVHYAV
VGEPSSGKVLGDVIKNGRRGSITGELYIEGVQGHVAYPHLAENPVHTSLNFLTELTTYQWDNGNEFFPPTSLQIANIKAG
TGSNNVIPGELYVQFNLRYCTEVTDEIIKNKVAEMLAKHQLKHRISWNLSGQPFLAGNGELVKATVQAVENVTKITPRLD
TSGGTSDGRFIALMGAEVVEFGPLNATIHKVNECVSVEDLGKCGEVYYHILERLLKS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=63.2, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1790395, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789236, Length=389, Percent_Identity=22.6221079691517, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17551016, Length=219, Percent_Identity=25.5707762557078, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_ACTP2 (A3N3G9)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054560.1
- ProteinModelPortal:   A3N3G9
- SMR:   A3N3G9
- STRING:   A3N3G9
- MEROPS:   M20.010
- GeneID:   4849441
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1873
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   A3N3G9
- ProtClustDB:   PRK13009
- BioCyc:   APLE416269:APL_1873-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41029; Mature: 41029

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNIINLAQDLIRRPSVSPDDQGCQQVIAERLAQLGFTLEWLPFGDTLNLWATHGTQDP
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCC
CVVFAGHTDVVPVGDETQWQYPPFSAEIVDGTLYGRGAADMKGSLAALVIAAETFVKNNP
EEEEECCCCEEECCCCCCCCCCCCCCEEECCEEECCCCCCCCCCHHHHHHHHHHHHCCCC
NHKGKIALLITSDEEAAAKAGTVKVVETLMARQEAVHYAVVGEPSSGKVLGDVIKNGRRG
CCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHCCCCCC
SITGELYIEGVQGHVAYPHLAENPVHTSLNFLTELTTYQWDNGNEFFPPTSLQIANIKAG
CEEEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECC
TGSNNVIPGELYVQFNLRYCTEVTDEIIKNKVAEMLAKHQLKHRISWNLSGQPFLAGNGE
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCH
LVKATVQAVENVTKITPRLDTSGGTSDGRFIALMGAEVVEFGPLNATIHKVNECVSVEDL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEVYYHILERLLKS
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHNIINLAQDLIRRPSVSPDDQGCQQVIAERLAQLGFTLEWLPFGDTLNLWATHGTQDP
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCC
CVVFAGHTDVVPVGDETQWQYPPFSAEIVDGTLYGRGAADMKGSLAALVIAAETFVKNNP
EEEEECCCCEEECCCCCCCCCCCCCCEEECCEEECCCCCCCCCCHHHHHHHHHHHHCCCC
NHKGKIALLITSDEEAAAKAGTVKVVETLMARQEAVHYAVVGEPSSGKVLGDVIKNGRRG
CCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHCCCCCC
SITGELYIEGVQGHVAYPHLAENPVHTSLNFLTELTTYQWDNGNEFFPPTSLQIANIKAG
CEEEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECC
TGSNNVIPGELYVQFNLRYCTEVTDEIIKNKVAEMLAKHQLKHRISWNLSGQPFLAGNGE
CCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCH
LVKATVQAVENVTKITPRLDTSGGTSDGRFIALMGAEVVEFGPLNATIHKVNECVSVEDL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEVYYHILERLLKS
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA