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The map label for this gene is cysN [H]

Identifier: 126209306

GI number: 126209306

Start: 2075287

End: 2076018

Strand: Reverse

Name: cysN [H]

Synonym: APL_1844

Alternate gene names: 126209306

Gene position: 2076018-2075287 (Counterclockwise)

Preceding gene: 126209311

Following gene: 126209305

Centisome position: 91.27

GC content: 45.36

Gene sequence:

>732_bases
GTGCCGGTTTCCGCTTTGCAAGGCGATAATATCGTGCATAAAAGCGAACGCACGCCTTGGTATGCGGGAGAGCCGTTACT
CACTATTTTAGAAAACTTACCAAGCAATGAGAATTTATCGGAACGAGCAGAAGATTTTCATTTCCCGGTACAGCTAGTCA
GTCGTTTAGATCAAGACAAAGCGGACGATTTCCGAGGTTATCAAGGCAGAATCGAAGCCGGGTCGGTTAGCGTAGGCGAC
ACGATTCGCATTGAACCGAACGGCTACACCTCGACCGTATCGGAAATTATTAGCCCTAACGGTATCGTACAAAAAGCGGT
TGCCGGTGAGCAGGTAACGATTCGTTTAGCAGATGATATTGATATTTCGCGCGGCGATACTTTTGTGGCACAACATTCTC
TGATTACCGCCACCAAACGCTTAACTGCTACGGTATGTTGGTTTGACCAACGTGCGTTAAATCCGGCACGTAAATATTTA
CTGAAGCACACCACACAAACCGTATTTGCCAAAGTGACCGCAGTTGATCACGTGCTTGATGTAAAAACCTTGAGTAATTC
GGCGGAAGCGGACAGCTTAAAATTAAATGATATTGGTGAGTTACAACTTAGCTTACAAAAGCCGATTACCGCTACCACTT
ATGCACAAAATCTTTCGACCGGATCATTTATTTTAATTGACGAGGCGACCTATCACACGGTAGCGGCAGGTATGATTCTT
GCTACCGAATAA

Upstream 100 bases:

>100_bases
CAAAATGGATTTGCTTGGTTTTGATGTGGCAAAATTTGAAGCAATTACGACCGCTTACTGCAAGTTAGCCGATCAGCTTG
GTATTCAACAGGTGCAATTT

Downstream 100 bases:

>100_bases
GATTCACCTTAAGAACAAGCGGTCAAAATTTGTTAATTTTTTGCAAATTTAGACCGCTTTTTTATGCACTTTATGTTTAT
AAACAAATAGCAAAATCATT

Product: sulphate adenylate transferase subunit 1

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPVSALQGDNIVHKSERTPWYAGEPLLTILENLPSNENLSERAEDFHFPVQLVSRLDQDKADDFRGYQGRIEAGSVSVGD
TIRIEPNGYTSTVSEIISPNGIVQKAVAGEQVTIRLADDIDISRGDTFVAQHSLITATKRLTATVCWFDQRALNPARKYL
LKHTTQTVFAKVTAVDHVLDVKTLSNSAEADSLKLNDIGELQLSLQKPITATTYAQNLSTGSFILIDEATYHTVAAGMIL
ATE

Sequences:

>Translated_243_residues
MPVSALQGDNIVHKSERTPWYAGEPLLTILENLPSNENLSERAEDFHFPVQLVSRLDQDKADDFRGYQGRIEAGSVSVGD
TIRIEPNGYTSTVSEIISPNGIVQKAVAGEQVTIRLADDIDISRGDTFVAQHSLITATKRLTATVCWFDQRALNPARKYL
LKHTTQTVFAKVTAVDHVLDVKTLSNSAEADSLKLNDIGELQLSLQKPITATTYAQNLSTGSFILIDEATYHTVAAGMIL
ATE
>Mature_242_residues
PVSALQGDNIVHKSERTPWYAGEPLLTILENLPSNENLSERAEDFHFPVQLVSRLDQDKADDFRGYQGRIEAGSVSVGDT
IRIEPNGYTSTVSEIISPNGIVQKAVAGEQVTIRLADDIDISRGDTFVAQHSLITATKRLTATVCWFDQRALNPARKYLL
KHTTQTVFAKVTAVDHVLDVKTLSNSAEADSLKLNDIGELQLSLQKPITATTYAQNLSTGSFILIDEATYHTVAAGMILA
TE

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Escherichia coli, GI1789108, Length=239, Percent_Identity=36.4016736401674, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 26598; Mature: 26467

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSALQGDNIVHKSERTPWYAGEPLLTILENLPSNENLSERAEDFHFPVQLVSRLDQDK
CCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHCCCCC
ADDFRGYQGRIEAGSVSVGDTIRIEPNGYTSTVSEIISPNGIVQKAVAGEQVTIRLADDI
CHHCCCCCCEEECCCEECCCEEEECCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEECCC
DISRGDTFVAQHSLITATKRLTATVCWFDQRALNPARKYLLKHTTQTVFAKVTAVDHVLD
CCCCCCEEEHHHHHHHHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VKTLSNSAEADSLKLNDIGELQLSLQKPITATTYAQNLSTGSFILIDEATYHTVAAGMIL
HHHHCCCCCCCCEECCCCCCEEEEECCCCEEHHHHHCCCCCCEEEEECCHHHHHHHCEEE
ATE
ECC
>Mature Secondary Structure 
PVSALQGDNIVHKSERTPWYAGEPLLTILENLPSNENLSERAEDFHFPVQLVSRLDQDK
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHCCCCC
ADDFRGYQGRIEAGSVSVGDTIRIEPNGYTSTVSEIISPNGIVQKAVAGEQVTIRLADDI
CHHCCCCCCEEECCCEECCCEEEECCCCCHHHHHHHHCCCCHHHHHCCCCEEEEEEECCC
DISRGDTFVAQHSLITATKRLTATVCWFDQRALNPARKYLLKHTTQTVFAKVTAVDHVLD
CCCCCCEEEHHHHHHHHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VKTLSNSAEADSLKLNDIGELQLSLQKPITATTYAQNLSTGSFILIDEATYHTVAAGMIL
HHHHCCCCCCCCEECCCCCCEEEEECCCCEEHHHHHCCCCCCEEEEECCHHHHHHHCEEE
ATE
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]