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The map label for this gene is rpoH [H]

Identifier: 126209296

GI number: 126209296

Start: 2056980

End: 2057930

Strand: Reverse

Name: rpoH [H]

Synonym: APL_1834

Alternate gene names: 126209296

Gene position: 2057930-2056980 (Counterclockwise)

Preceding gene: 126209297

Following gene: 126209293

Centisome position: 90.48

GC content: 45.22

Gene sequence:

>951_bases
ATGAAAAAATTCGATGAACATGAGATTGAAGATGCGGAAATTATCGAGCCGGGCGAAATAGAAGTGACGGAAAGCGATAA
TCAGCCGACTGAGGCAATGCACCCCGCTTTGGTACAGGGCGGAATGCCGGTGCCGCAAGGTAATCTCGACAGCTATATTC
GTATGGCGAATCAATATCCGATTCTCACCGCCGAACAAGAAAAGGAATTGGCGGAACGCTATTACTATGATGAAGATGTT
GAGGCGGCAAAACAGCTAATTCTATCGCATATGCGTTTTGTGATTCATATCGCCAGAGGTTATTTGGGATACGGTTTACC
GCTTGCCGACTTAATTCAAGAAGGTAATATCGGCTTAATGAAAGCGGTAAAACGGTTTGACCCGAATGTCGGCGTGCGCT
TGGTATCTTTTGCGGTACACTGGGTGAAAGCGGAAATTCACGAATACGTATTAAAAAACTGGCGTATCGTGAAAGTCGCT
ACGACCAAAGCGCAGCGTAAACTCTTTTTCAATCTGCGCAAAAATAAAAACCGCTTGGCTTGGTTCAACGAGGAAGAAAT
CCAAAAAGTCGCCGAAGATTTGGGTGTTTCGCCCGAAGAAGTGCGAGAAATGGAATCCCGTATGACCGGGCAAGATCTCG
GTTTTGATTTGCCGGTCGGCGAAGACGATGACGAAGTTTATACGCCGTCAATGTATTTGGAAGATAACAGCTCTAACTTT
GCCGATGACTTGGAAGACGAGCAATACAACGGACAAGCTACCGCTCAATTAGCTTATGCCTTAGCAACACTGGACGAACG
TAGCCAAGATATTATTAAAACTCGTTGGTTGGATGAAGAAAAAGCGACTTTACAAGATCTGGCGGAAAAATACGGTATTT
CCGCCGAGCGCGTTCGCCAATTGGAAAATGCGGCATTGAAGAAAATCAAAGATGCGATAACCTTTGAATAG

Upstream 100 bases:

>100_bases
TCGGTTTTTTTATTTGAATCTAATCTGCTCGTAAGAAAAACAGACGAGAAAGTTCTAACGCTGTTATAATGTAAATTAAA
TAATTAGCCGGAGAGATAGC

Downstream 100 bases:

>100_bases
CTTGATTTTGAAAAAATAATAAGCGGTCATTTTTACCGAATTTTTAGTAAAAATGACCGCTTATTTTTTATGCTGACTAA
ATAAATTCTTTTATCGGGCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKKFDEHEIEDAEIIEPGEIEVTESDNQPTEAMHPALVQGGMPVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDV
EAAKQLILSHMRFVIHIARGYLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVKVA
TTKAQRKLFFNLRKNKNRLAWFNEEEIQKVAEDLGVSPEEVREMESRMTGQDLGFDLPVGEDDDEVYTPSMYLEDNSSNF
ADDLEDEQYNGQATAQLAYALATLDERSQDIIKTRWLDEEKATLQDLAEKYGISAERVRQLENAALKKIKDAITFE

Sequences:

>Translated_316_residues
MKKFDEHEIEDAEIIEPGEIEVTESDNQPTEAMHPALVQGGMPVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDV
EAAKQLILSHMRFVIHIARGYLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVKVA
TTKAQRKLFFNLRKNKNRLAWFNEEEIQKVAEDLGVSPEEVREMESRMTGQDLGFDLPVGEDDDEVYTPSMYLEDNSSNF
ADDLEDEQYNGQATAQLAYALATLDERSQDIIKTRWLDEEKATLQDLAEKYGISAERVRQLENAALKKIKDAITFE
>Mature_316_residues
MKKFDEHEIEDAEIIEPGEIEVTESDNQPTEAMHPALVQGGMPVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDV
EAAKQLILSHMRFVIHIARGYLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVKVA
TTKAQRKLFFNLRKNKNRLAWFNEEEIQKVAEDLGVSPEEVREMESRMTGQDLGFDLPVGEDDDEVYTPSMYLEDNSSNF
ADDLEDEQYNGQATAQLAYALATLDERSQDIIKTRWLDEEKATLQDLAEKYGISAERVRQLENAALKKIKDAITFE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=271, Percent_Identity=66.789667896679, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789098, Length=315, Percent_Identity=27.9365079365079, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789448, Length=254, Percent_Identity=27.1653543307087, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36251; Mature: 36251

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFDEHEIEDAEIIEPGEIEVTESDNQPTEAMHPALVQGGMPVPQGNLDSYIRMANQYP
CCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
ILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARGYLGYGLPLADLIQEGNIGLM
CEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHH
KAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKNKNRLA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCEE
WFNEEEIQKVAEDLGVSPEEVREMESRMTGQDLGFDLPVGEDDDEVYTPSMYLEDNSSNF
ECCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEEEECCCCCC
ADDLEDEQYNGQATAQLAYALATLDERSQDIIKTRWLDEEKATLQDLAEKYGISAERVRQ
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
LENAALKKIKDAITFE
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFDEHEIEDAEIIEPGEIEVTESDNQPTEAMHPALVQGGMPVPQGNLDSYIRMANQYP
CCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
ILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARGYLGYGLPLADLIQEGNIGLM
CEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHH
KAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKNKNRLA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHCCCCCEE
WFNEEEIQKVAEDLGVSPEEVREMESRMTGQDLGFDLPVGEDDDEVYTPSMYLEDNSSNF
ECCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEEEECCCCCC
ADDLEDEQYNGQATAQLAYALATLDERSQDIIKTRWLDEEKATLQDLAEKYGISAERVRQ
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
LENAALKKIKDAITFE
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]