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The map label for this gene is macA [H]

Identifier: 126209276

GI number: 126209276

Start: 2036534

End: 2037310

Strand: Reverse

Name: macA [H]

Synonym: APL_1814

Alternate gene names: 126209276

Gene position: 2037310-2036534 (Counterclockwise)

Preceding gene: 126209277

Following gene: 126209274

Centisome position: 89.57

GC content: 37.45

Gene sequence:

>777_bases
TTGCAAGCGACTGAACAAGTAGATATTGGTGCACAAGTATCTGGGCAGATTAAGCATATTTTAGTACAAGAAGGACAGAA
GGTTAAAAAAGGTGAGCTATTAGCTGTAATTGATCCACGTCTGGCTGAAACGGAATTAAAACTAGCAAAAGCTGAGCTAG
CAAATGCTTCTGCTAATTTGGATACAAAAAAAATTAATCTTAAGCAACTGCAATCAGATTGGGAACGTCATCAACGTTTG
ATACGAACCAATGCGACAAGCCAAAAGGAAACAGAAGAAGCAAAAAGTAGATTAAATACGGCCAAAGCAGAACTTCAAAT
TGCGCAAAATAATCTAGATATCGCTAAAATCAGAGTGGAAAAAGCTGAAACCGAACTAGGATATACAGAAATTCGTTCTC
CACTTGATGCAACAGTAATTTCAGTATTTGCGCAAAATGGTCAAACTTTAGTCACCACCCAACAAGTACCAGTGCTGATG
AAATTAGCTAATATTGATACTATGAAAGTCAACACCAAAATTTCGGAAGCAGATGTAATTCATATACAAGCTGGTGCTCC
AACTTCCTTTACGCTATTGGGAGAGAGTGATTCCCAATTTCACGGGCAACTTGATATGGTAAAACTGGCTCCCGTTCATA
TTACAGACACTACAGAAAATACAAACAATGCAATTTATTATTATGCTACTTTCAAAGTGCCTAATCCTGATCATAGACTG
CGTATTTCTATGACTGGCCGCTGTACGATTATGTTAGATAAACGTGAAATGTGTTGA

Upstream 100 bases:

>100_bases
TCATATTTATATTTTGGGGGCGAAAACGAAAATTATCAAGAGATTATTTTTCACGTCGAACGAGGAAAATATTCAAAAAA
CAGTTATTGCTAGCGGCACA

Downstream 100 bases:

>100_bases
ATATTCCTTTATCAGCCCTAGGGGAAATGATTGTGCCGGAGCAATATTATGTCACGGTCTTGCGTAAACATGGCCAAAAA
GAGCAAGTTGTAGTGAGAAC

Product: macrolide-specific efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MQATEQVDIGAQVSGQIKHILVQEGQKVKKGELLAVIDPRLAETELKLAKAELANASANLDTKKINLKQLQSDWERHQRL
IRTNATSQKETEEAKSRLNTAKAELQIAQNNLDIAKIRVEKAETELGYTEIRSPLDATVISVFAQNGQTLVTTQQVPVLM
KLANIDTMKVNTKISEADVIHIQAGAPTSFTLLGESDSQFHGQLDMVKLAPVHITDTTENTNNAIYYYATFKVPNPDHRL
RISMTGRCTIMLDKREMC

Sequences:

>Translated_258_residues
MQATEQVDIGAQVSGQIKHILVQEGQKVKKGELLAVIDPRLAETELKLAKAELANASANLDTKKINLKQLQSDWERHQRL
IRTNATSQKETEEAKSRLNTAKAELQIAQNNLDIAKIRVEKAETELGYTEIRSPLDATVISVFAQNGQTLVTTQQVPVLM
KLANIDTMKVNTKISEADVIHIQAGAPTSFTLLGESDSQFHGQLDMVKLAPVHITDTTENTNNAIYYYATFKVPNPDHRL
RISMTGRCTIMLDKREMC
>Mature_258_residues
MQATEQVDIGAQVSGQIKHILVQEGQKVKKGELLAVIDPRLAETELKLAKAELANASANLDTKKINLKQLQSDWERHQRL
IRTNATSQKETEEAKSRLNTAKAELQIAQNNLDIAKIRVEKAETELGYTEIRSPLDATVISVFAQNGQTLVTTQQVPVLM
KLANIDTMKVNTKISEADVIHIQAGAPTSFTLLGESDSQFHGQLDMVKLAPVHITDTTENTNNAIYYYATFKVPNPDHRL
RISMTGRCTIMLDKREMC

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=252, Percent_Identity=36.5079365079365, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1789929, Length=249, Percent_Identity=26.1044176706827, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790519, Length=247, Percent_Identity=24.6963562753036, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789041, Length=241, Percent_Identity=27.3858921161826, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 28720; Mature: 28720

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQATEQVDIGAQVSGQIKHILVQEGQKVKKGELLAVIDPRLAETELKLAKAELANASANL
CCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCC
DTKKINLKQLQSDWERHQRLIRTNATSQKETEEAKSRLNTAKAELQIAQNNLDIAKIRVE
CEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEE
KAETELGYTEIRSPLDATVISVFAQNGQTLVTTQQVPVLMKLANIDTMKVNTKISEADVI
HHHHHCCHHHHCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEECEECCCEEE
HIQAGAPTSFTLLGESDSQFHGQLDMVKLAPVHITDTTENTNNAIYYYATFKVPNPDHRL
EEECCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCCCEE
RISMTGRCTIMLDKREMC
EEEECCEEEEEECCCCCC
>Mature Secondary Structure
MQATEQVDIGAQVSGQIKHILVQEGQKVKKGELLAVIDPRLAETELKLAKAELANASANL
CCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCC
DTKKINLKQLQSDWERHQRLIRTNATSQKETEEAKSRLNTAKAELQIAQNNLDIAKIRVE
CEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEE
KAETELGYTEIRSPLDATVISVFAQNGQTLVTTQQVPVLMKLANIDTMKVNTKISEADVI
HHHHHCCHHHHCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEECEECCCEEE
HIQAGAPTSFTLLGESDSQFHGQLDMVKLAPVHITDTTENTNNAIYYYATFKVPNPDHRL
EEECCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCCCEE
RISMTGRCTIMLDKREMC
EEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]