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The map label for this gene is fmt

Identifier: 126209051

GI number: 126209051

Start: 1814950

End: 1815900

Strand: Reverse

Name: fmt

Synonym: APL_1587

Alternate gene names: 126209051

Gene position: 1815900-1814950 (Counterclockwise)

Preceding gene: 126209052

Following gene: 126209050

Centisome position: 79.84

GC content: 44.58

Gene sequence:

>951_bases
ATGTCAAAACTTAATATTATTTTTGCCGGCACACCTGATTTTGCCGCACAACACTTACAAGCATTATTAGATTCAGAACA
TAATGTGATTGCAGTTTATACCCAACCGGACAAACCTGCCGGCAGAGGTAAAAAACTGCAAGCCAGCCCGGTTAAACAAT
TAGCCGAACAACATAATATTCCGGTATATCAACCAAAATCTTTACGTAAAGAAGACGCACAAGCGGAATTAAAAGCACTG
AATGCCGATGTAATGGTTGTAGTGGCTTACGGTTTAATCTTACCGGAAGCGGTACTGAATGCGCCGAAATACGGTTGTTT
AAATGTGCACGGTTCGCTGTTACCACGCTGGCGTGGAGCGGCACCGATTCAACGCTCGATTTGGGCGGGCGATCAGGAAA
CTGGCGTGACCATTATGCAAATGGATATCGGCTTAGATACCGGCGATATGTTACATAAAGTTACCACACCGATTGCCGCA
GACGAAACCTCCGCTTCGTTATATGCCAAATTAGCCGAACTTGCACCGCCGGCGTTATTAGAGGTGTTAAACGGGTTAGA
AAGCCAAGCGTTTAAAGCGGAAAAACAAGATGATGCGCTTTCCAACTATGCGGAAAAACTTTCAAAAGAAGAAGCAAAAT
TAGACTGGAATTTAACCGCTTGTCAGCTGGAACGCAATATTCGTGCATTTAACCCATGGCCTATTAGCTTTCTCACATTA
GAGGTGGACGGTGTAGAGCAAAGCGTGAAAGTGTATCAAGCGAATGTGTTACCGCATCAAGCTAAAGCGGCAGGTACGGT
CTTACAAGCGGATAAAAACGGGATTCAAATTGCAACACAAGAAGGCGTGTTAAATATTACTCAGTTACAACCTTCCGGCA
AAAAACCGATGTCCGTGCAAGACTTCCTTAACGGGCGTGCCGATTGGTTTGCAGTGGGAAAACAACTCTAA

Upstream 100 bases:

>100_bases
AACCTGCGTATTTCGCGATTAAAAATCTTTATCACCAAACACAGTTGCCGTATTTCTTCTGTTACAATTCCATTATCCAT
TCACTTATCAACGAAAAATT

Downstream 100 bases:

>100_bases
CAAATAAAAACTATCGGATACGGAAAACATAACGCACACGCCTAATAATTATTCCATTAGCGTTGTGTTTTCCGTCATAA
CAGGAACACAACATGCCAAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQHNIPVYQPKSLRKEDAQAELKAL
NADVMVVVAYGLILPEAVLNAPKYGCLNVHGSLLPRWRGAAPIQRSIWAGDQETGVTIMQMDIGLDTGDMLHKVTTPIAA
DETSASLYAKLAELAPPALLEVLNGLESQAFKAEKQDDALSNYAEKLSKEEAKLDWNLTACQLERNIRAFNPWPISFLTL
EVDGVEQSVKVYQANVLPHQAKAAGTVLQADKNGIQIATQEGVLNITQLQPSGKKPMSVQDFLNGRADWFAVGKQL

Sequences:

>Translated_316_residues
MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQHNIPVYQPKSLRKEDAQAELKAL
NADVMVVVAYGLILPEAVLNAPKYGCLNVHGSLLPRWRGAAPIQRSIWAGDQETGVTIMQMDIGLDTGDMLHKVTTPIAA
DETSASLYAKLAELAPPALLEVLNGLESQAFKAEKQDDALSNYAEKLSKEEAKLDWNLTACQLERNIRAFNPWPISFLTL
EVDGVEQSVKVYQANVLPHQAKAAGTVLQADKNGIQIATQEGVLNITQLQPSGKKPMSVQDFLNGRADWFAVGKQL
>Mature_315_residues
SKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQHNIPVYQPKSLRKEDAQAELKALN
ADVMVVVAYGLILPEAVLNAPKYGCLNVHGSLLPRWRGAAPIQRSIWAGDQETGVTIMQMDIGLDTGDMLHKVTTPIAAD
ETSASLYAKLAELAPPALLEVLNGLESQAFKAEKQDDALSNYAEKLSKEEAKLDWNLTACQLERNIRAFNPWPISFLTLE
VDGVEQSVKVYQANVLPHQAKAAGTVLQADKNGIQIATQEGVLNITQLQPSGKKPMSVQDFLNGRADWFAVGKQL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=317, Percent_Identity=31.5457413249211, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI21614513, Length=291, Percent_Identity=26.4604810996564, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI238814322, Length=323, Percent_Identity=27.2445820433437, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=64.0127388535032, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1788589, Length=288, Percent_Identity=28.125, Blast_Score=110, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=275, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319458, Length=165, Percent_Identity=32.1212121212121, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=34.6491228070175, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24585660, Length=292, Percent_Identity=27.0547945205479, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_ACTP2 (A3N2N5)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054276.1
- STRING:   A3N2N5
- GeneID:   4850457
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1587
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   A3N2N5
- ProtClustDB:   PRK00005
- BioCyc:   APLE416269:APL_1587-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34436; Mature: 34305

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQHNI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVYQPKSLRKEDAQAELKALNADVMVVVAYGLILPEAVLNAPKYGCLNVHGSLLPRWRGA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
APIQRSIWAGDQETGVTIMQMDIGLDTGDMLHKVTTPIAADETSASLYAKLAELAPPALL
CCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
EVLNGLESQAFKAEKQDDALSNYAEKLSKEEAKLDWNLTACQLERNIRAFNPWPISFLTL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHEEHHCCCCCCCCCCEEEEEE
EVDGVEQSVKVYQANVLPHQAKAAGTVLQADKNGIQIATQEGVLNITQLQPSGKKPMSVQ
EECCCHHHHHHEECCCCCCHHHHCCEEEEECCCCEEEEECCCEEEEEEECCCCCCCCCHH
DFLNGRADWFAVGKQL
HHHCCCCHHHHCCCCC
>Mature Secondary Structure 
SKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGKKLQASPVKQLAEQHNI
CCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVYQPKSLRKEDAQAELKALNADVMVVVAYGLILPEAVLNAPKYGCLNVHGSLLPRWRGA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
APIQRSIWAGDQETGVTIMQMDIGLDTGDMLHKVTTPIAADETSASLYAKLAELAPPALL
CCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
EVLNGLESQAFKAEKQDDALSNYAEKLSKEEAKLDWNLTACQLERNIRAFNPWPISFLTL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHEEHHCCCCCCCCCCEEEEEE
EVDGVEQSVKVYQANVLPHQAKAAGTVLQADKNGIQIATQEGVLNITQLQPSGKKPMSVQ
EECCCHHHHHHEECCCCCCHHHHCCEEEEECCCCEEEEECCCEEEEEEECCCCCCCCCHH
DFLNGRADWFAVGKQL
HHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA