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The map label for this gene is vacB [H]

Identifier: 126208942

GI number: 126208942

Start: 1692326

End: 1694683

Strand: Reverse

Name: vacB [H]

Synonym: APL_1478

Alternate gene names: 126208942

Gene position: 1694683-1692326 (Counterclockwise)

Preceding gene: 126208943

Following gene: 126208941

Centisome position: 74.51

GC content: 45.04

Gene sequence:

>2358_bases
ATGATAATTGATCCGAATTACCAACAAGAAAGCGAAAAGTACGAAAATCCCGTACCGAGCAGAGAATTTATTTTACAAAC
CATTCGAGCATTCGATGCGCCGATGAGTCGTGAAGAATTACTCGAAGCTTTCCATATTTATGATGAAGAACGTATCGAAG
GCGTTCGCCGTCGCTTACGAGCGATGGAAAACGACGGGCAATTAATTTTTACCAAAGGTAAACGTTATGCGTTACCGGAA
AAAATGGATTTGCTGAAAGGTACGGTTATCGGTCATCGTGACGGCTACGGTTTTCTGCAAGTGGAAGGCGGCGAAAAAAG
TGACGATTGGTTTATTCCGAATAGCCAAATGACACGCGTGATGCACGGTGACTTCGTGCTTGCCCAACCGAACGGCACCG
ATCGCCGAGGTCGCCGTGAAGTACGAATCGTTCGCGTATTGGAAGCACGTAAAAAACAAATTGTCGGTCGTTTCTTCCTT
GAAAGCGGTATCGGCTTCGTAGTACCGGACGATAGCCGTATCAGCCAAGATATTTTAATTCCCGACGAACACCGTATGGG
TGCAAGAATGGGACAAGTGGTTGTGGTCGAACTGCAAGAACGTAAAGCCGGTTTTACTCGTCCGGTCGGCTTTATTACCG
AAATCTTAGGCGATAATCTTGCACCGGGGATGGAAATCGAAATCGCTCTACGTAACCACGACATTCCGCACGTTTGGCCG
GAAGGCGTAGAAAAACAAATTCGCCAATTCAGCGAAGAAGTGCCGGAAGAAGCGAAAGCTGGCCGTGTAGATCTGCGTGA
TTTACCGTTAATAACGATTGACGGGGAAGACGCACGAGATTTTGACGACGCCGTCTTTGCCAGAAAAGAAGGCGACGGTT
GGCGTTTATGGGTAGCGATTGCCGATGTGAGTTATTACGTTCGTCCGAAAACCGCACTGGATTTGGAAGCGGCGAATCGC
GGCAATTCGGTTTACTTCCCGAATCGAGTGATCCCGATGTTACCGGAAATTCTGTCAAACGGTTTATGTTCATTAAATCC
GCAAGTGGACAGATTATGTTTGGTGGCAGAAATGACCGTATCGAAAAAAGGCGAACTGACCGGCTATCAATTCTATCAAG
CGGTAATGAACTCGCACGCCCGCTTAACTTATACTAAAGTTTGGAAAATATTAGAAGGTGACGAAGCGTTACGTGAGCGT
TATGCAACACTGGTACCGCATTTGGAAACTTTAAATGAGATGTTTAAAGTGTTGCAAAACACTCGTCGTCATCGAGGTGC
GATTGAATTTGAAACGATTGAAAACCAATTTATTTTCAATCCGCAAGGCAGAATCGAACGTATCGAGCCGCTAATTCGTA
ACGATGCGCACAAAATCATCGAAGAATGTATGATTTTAGCGAATATTGCCGCCGCTCGTCTTGTCGAAAAAGCCAATGAA
CCGGCGTTATTCCGTATTCACGATAAACCGAGCGAAGAAAAGCTCACTAGTTTTAAATCCTTTATCAAAGAATGCGGACT
CACTTGGGACGTCGGGCTTGATCCGACACCAAAAGATTATGCCGTATTACTCGAACAACTCGCCGAACGTCCGGATCGCG
AATTGATTCAAACTATGTTACTGCGCTCGCTCAAACAAGCGGTCTATTCAGCGGATAACGTCGGACATTTCGGTTTGGCT
TTAACCGAATACGCCCACTTTACTTCACCTATTCGCCGTTATCCGGATTTGCTGTTACACCGTGCAATTAAATACTTGAT
TGAAAAGGGAAAAGGCAATACCAGACATTATACCGATTGCGGCGGTTATCACTATAAATTGGACGATATCGACCAATTCG
GCGAGAAATGTTCGGCAGCCGAACGTCGGGCGGACGAAGCGACCCGTGAAGTGGCGGATTGGTTGAAATGCGAATTTATG
CAAGATCATCTCGGCGAAGAGTTTGAAGGTGTGATTTCAAGCGTAACCGGTTTCGGTTTATTCGTTAAAATCAACGAGTT
ATTGATCGACGGCTTGGTGCATATTTCAACATTAGACAATGATTACTATCATTATGATGCGGACAGACAACGTTTAGTCG
GCGGCAGCGGTGTGATTTACCGCTTAGGTGATCCGGTTAAAGTGAAAGTAATCAATGTGAATTTAGACGATAAGAAAATT
GACTTTGCTTTAATCGATAATCTGCGTAAAGGCATTGCCGTCGGTAAAACCGCCAAACAAAAAGCAAAGAAAAATGCCGG
CGATAAACCGATTTTTAAAGAGCCTAAAACAATCAAATCGGCAAAAGGTAAATCTAAAGCTAAGGGAAAAGCGACCAAAA
CAGCCAAGAAATCGTCCGGTCGCCAACGTAAGAAATAG

Upstream 100 bases:

>100_bases
AAAGTCCGCCTTTGGCTCAGTAACTTAACTTAATTCGAGCGGTCTGATTTGTAAATTTTTCTGCAAATCAGACCGCTTTA
ACATATATAAAGAGTACATT

Downstream 100 bases:

>100_bases
CGAATAAGCGGTCAAATTTGCAAAACTTCTTACAAATTCTACCGCTTCCCAAATTCTCCGTTTAAACTAATATAGCCTAT
TCTTTTAGGCTATTTTTATC

Product: ribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 785; Mature: 785

Protein sequence:

>785_residues
MIIDPNYQQESEKYENPVPSREFILQTIRAFDAPMSREELLEAFHIYDEERIEGVRRRLRAMENDGQLIFTKGKRYALPE
KMDLLKGTVIGHRDGYGFLQVEGGEKSDDWFIPNSQMTRVMHGDFVLAQPNGTDRRGRREVRIVRVLEARKKQIVGRFFL
ESGIGFVVPDDSRISQDILIPDEHRMGARMGQVVVVELQERKAGFTRPVGFITEILGDNLAPGMEIEIALRNHDIPHVWP
EGVEKQIRQFSEEVPEEAKAGRVDLRDLPLITIDGEDARDFDDAVFARKEGDGWRLWVAIADVSYYVRPKTALDLEAANR
GNSVYFPNRVIPMLPEILSNGLCSLNPQVDRLCLVAEMTVSKKGELTGYQFYQAVMNSHARLTYTKVWKILEGDEALRER
YATLVPHLETLNEMFKVLQNTRRHRGAIEFETIENQFIFNPQGRIERIEPLIRNDAHKIIEECMILANIAAARLVEKANE
PALFRIHDKPSEEKLTSFKSFIKECGLTWDVGLDPTPKDYAVLLEQLAERPDRELIQTMLLRSLKQAVYSADNVGHFGLA
LTEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRHYTDCGGYHYKLDDIDQFGEKCSAAERRADEATREVADWLKCEFM
QDHLGEEFEGVISSVTGFGLFVKINELLIDGLVHISTLDNDYYHYDADRQRLVGGSGVIYRLGDPVKVKVINVNLDDKKI
DFALIDNLRKGIAVGKTAKQKAKKNAGDKPIFKEPKTIKSAKGKSKAKGKATKTAKKSSGRQRKK

Sequences:

>Translated_785_residues
MIIDPNYQQESEKYENPVPSREFILQTIRAFDAPMSREELLEAFHIYDEERIEGVRRRLRAMENDGQLIFTKGKRYALPE
KMDLLKGTVIGHRDGYGFLQVEGGEKSDDWFIPNSQMTRVMHGDFVLAQPNGTDRRGRREVRIVRVLEARKKQIVGRFFL
ESGIGFVVPDDSRISQDILIPDEHRMGARMGQVVVVELQERKAGFTRPVGFITEILGDNLAPGMEIEIALRNHDIPHVWP
EGVEKQIRQFSEEVPEEAKAGRVDLRDLPLITIDGEDARDFDDAVFARKEGDGWRLWVAIADVSYYVRPKTALDLEAANR
GNSVYFPNRVIPMLPEILSNGLCSLNPQVDRLCLVAEMTVSKKGELTGYQFYQAVMNSHARLTYTKVWKILEGDEALRER
YATLVPHLETLNEMFKVLQNTRRHRGAIEFETIENQFIFNPQGRIERIEPLIRNDAHKIIEECMILANIAAARLVEKANE
PALFRIHDKPSEEKLTSFKSFIKECGLTWDVGLDPTPKDYAVLLEQLAERPDRELIQTMLLRSLKQAVYSADNVGHFGLA
LTEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRHYTDCGGYHYKLDDIDQFGEKCSAAERRADEATREVADWLKCEFM
QDHLGEEFEGVISSVTGFGLFVKINELLIDGLVHISTLDNDYYHYDADRQRLVGGSGVIYRLGDPVKVKVINVNLDDKKI
DFALIDNLRKGIAVGKTAKQKAKKNAGDKPIFKEPKTIKSAKGKSKAKGKATKTAKKSSGRQRKK
>Mature_785_residues
MIIDPNYQQESEKYENPVPSREFILQTIRAFDAPMSREELLEAFHIYDEERIEGVRRRLRAMENDGQLIFTKGKRYALPE
KMDLLKGTVIGHRDGYGFLQVEGGEKSDDWFIPNSQMTRVMHGDFVLAQPNGTDRRGRREVRIVRVLEARKKQIVGRFFL
ESGIGFVVPDDSRISQDILIPDEHRMGARMGQVVVVELQERKAGFTRPVGFITEILGDNLAPGMEIEIALRNHDIPHVWP
EGVEKQIRQFSEEVPEEAKAGRVDLRDLPLITIDGEDARDFDDAVFARKEGDGWRLWVAIADVSYYVRPKTALDLEAANR
GNSVYFPNRVIPMLPEILSNGLCSLNPQVDRLCLVAEMTVSKKGELTGYQFYQAVMNSHARLTYTKVWKILEGDEALRER
YATLVPHLETLNEMFKVLQNTRRHRGAIEFETIENQFIFNPQGRIERIEPLIRNDAHKIIEECMILANIAAARLVEKANE
PALFRIHDKPSEEKLTSFKSFIKECGLTWDVGLDPTPKDYAVLLEQLAERPDRELIQTMLLRSLKQAVYSADNVGHFGLA
LTEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRHYTDCGGYHYKLDDIDQFGEKCSAAERRADEATREVADWLKCEFM
QDHLGEEFEGVISSVTGFGLFVKINELLIDGLVHISTLDNDYYHYDADRQRLVGGSGVIYRLGDPVKVKVINVNLDDKKI
DFALIDNLRKGIAVGKTAKQKAKKNAGDKPIFKEPKTIKSAKGKSKAKGKATKTAKKSSGRQRKK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=603, Percent_Identity=29.8507462686567, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI190014625, Length=603, Percent_Identity=29.8507462686567, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI134288890, Length=363, Percent_Identity=30.5785123966942, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI19115966, Length=578, Percent_Identity=25.2595155709343, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI219521928, Length=578, Percent_Identity=25.2595155709343, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI156105693, Length=219, Percent_Identity=29.6803652968037, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI156105695, Length=219, Percent_Identity=29.6803652968037, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI87082383, Length=742, Percent_Identity=65.7681940700809, Blast_Score=1012, Evalue=0.0,
Organism=Escherichia coli, GI1787542, Length=658, Percent_Identity=27.5075987841945, Blast_Score=169, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI212645896, Length=568, Percent_Identity=27.6408450704225, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17553506, Length=520, Percent_Identity=27.5, Blast_Score=162, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6324552, Length=639, Percent_Identity=24.8826291079812, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24649634, Length=669, Percent_Identity=26.1584454409567, Blast_Score=172, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24654592, Length=353, Percent_Identity=29.7450424929178, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19922976, Length=361, Percent_Identity=29.3628808864266, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24654597, Length=361, Percent_Identity=29.3628808864266, Blast_Score=132, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668 [H]

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 89518; Mature: 89518

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDPNYQQESEKYENPVPSREFILQTIRAFDAPMSREELLEAFHIYDEERIEGVRRRLR
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AMENDGQLIFTKGKRYALPEKMDLLKGTVIGHRDGYGFLQVEGGEKSDDWFIPNSQMTRV
HHCCCCCEEEECCCCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCCCEECCCHHHHHE
MHGDFVLAQPNGTDRRGRREVRIVRVLEARKKQIVGRFFLESGIGFVVPDDSRISQDILI
EECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PDEHRMGARMGQVVVVELQERKAGFTRPVGFITEILGDNLAPGMEIEIALRNHDIPHVWP
CCCHHHHHCCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
EGVEKQIRQFSEEVPEEAKAGRVDLRDLPLITIDGEDARDFDDAVFARKEGDGWRLWVAI
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHEEECCCCEEEEEEE
ADVSYYVRPKTALDLEAANRGNSVYFPNRVIPMLPEILSNGLCSLNPQVDRLCLVAEMTV
ECCEEEECCCCEEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SKKGELTGYQFYQAVMNSHARLTYTKVWKILEGDEALRERYATLVPHLETLNEMFKVLQN
CCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRRHRGAIEFETIENQFIFNPQGRIERIEPLIRNDAHKIIEECMILANIAAARLVEKANE
HHHHCCCEEEEECCCEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
PALFRIHDKPSEEKLTSFKSFIKECGLTWDVGLDPTPKDYAVLLEQLAERPDRELIQTML
CCEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LRSLKQAVYSADNVGHFGLALTEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRHYTDC
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
GGYHYKLDDIDQFGEKCSAAERRADEATREVADWLKCEFMQDHLGEEFEGVISSVTGFGL
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEE
FVKINELLIDGLVHISTLDNDYYHYDADRQRLVGGSGVIYRLGDPVKVKVINVNLDDKKI
EEEHHHHHHHHHHEEEEECCCCEEECCCHHHEECCCEEEEECCCCEEEEEEEECCCCCHH
DFALIDNLRKGIAVGKTAKQKAKKNAGDKPIFKEPKTIKSAKGKSKAKGKATKTAKKSSG
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCC
RQRKK
CCCCC
>Mature Secondary Structure
MIIDPNYQQESEKYENPVPSREFILQTIRAFDAPMSREELLEAFHIYDEERIEGVRRRLR
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AMENDGQLIFTKGKRYALPEKMDLLKGTVIGHRDGYGFLQVEGGEKSDDWFIPNSQMTRV
HHCCCCCEEEECCCCCCCCHHHHHHHCCEEECCCCCEEEEECCCCCCCCEECCCHHHHHE
MHGDFVLAQPNGTDRRGRREVRIVRVLEARKKQIVGRFFLESGIGFVVPDDSRISQDILI
EECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PDEHRMGARMGQVVVVELQERKAGFTRPVGFITEILGDNLAPGMEIEIALRNHDIPHVWP
CCCHHHHHCCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
EGVEKQIRQFSEEVPEEAKAGRVDLRDLPLITIDGEDARDFDDAVFARKEGDGWRLWVAI
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHEEECCCCEEEEEEE
ADVSYYVRPKTALDLEAANRGNSVYFPNRVIPMLPEILSNGLCSLNPQVDRLCLVAEMTV
ECCEEEECCCCEEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SKKGELTGYQFYQAVMNSHARLTYTKVWKILEGDEALRERYATLVPHLETLNEMFKVLQN
CCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRRHRGAIEFETIENQFIFNPQGRIERIEPLIRNDAHKIIEECMILANIAAARLVEKANE
HHHHCCCEEEEECCCEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
PALFRIHDKPSEEKLTSFKSFIKECGLTWDVGLDPTPKDYAVLLEQLAERPDRELIQTML
CCEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LRSLKQAVYSADNVGHFGLALTEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRHYTDC
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECC
GGYHYKLDDIDQFGEKCSAAERRADEATREVADWLKCEFMQDHLGEEFEGVISSVTGFGL
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCEE
FVKINELLIDGLVHISTLDNDYYHYDADRQRLVGGSGVIYRLGDPVKVKVINVNLDDKKI
EEEHHHHHHHHHHEEEEECCCCEEECCCHHHEECCCEEEEECCCCEEEEEEEECCCCCHH
DFALIDNLRKGIAVGKTAKQKAKKNAGDKPIFKEPKTIKSAKGKSKAKGKATKTAKKSSG
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHCC
RQRKK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]