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The map label for this gene is mpl [H]

Identifier: 126208913

GI number: 126208913

Start: 1661206

End: 1662570

Strand: Reverse

Name: mpl [H]

Synonym: APL_1449

Alternate gene names: 126208913

Gene position: 1662570-1661206 (Counterclockwise)

Preceding gene: 126208915

Following gene: 126208909

Centisome position: 73.1

GC content: 44.76

Gene sequence:

>1365_bases
ATGAGACAAAAACATATTCATATCTTGGGAATCTGCGGAACATTTATGGGTGGTGTCGCAATGATTGCTCGAGAACTCGG
CTATAAAGTAACGGGTTCGGACACTAACGTTTATCCACCGATGAGTACATTTTTAGAAAGCCACGGTATTGATATTATCC
CGAATTACGATGTCGCACAGTTGCAACCTGCACCGGATTTAGTGGTAATTGGGAATGCAATGAGCCGGGGCAATCCTTGC
GTAGAATATGTATTGGATAATCAGCTAAATTACACTTCAGGTCCGCAATGGTTACATGATCATCTGTTAAAAGATCGCTG
GGTGTTAGCCGTATCCGGTACGCACGGTAAAACCACTACTACCGGTATGTTAGCGTGGATTTTAGATCAAAATAATCTTG
ATGCCGGTTTCTTAATCGGCGGGGTTGCCGGTAATTTCGGTATTTCGGCGCGTGCCGGCTCAAGCAAATTCTTTGTAATT
GAAGCGGACGAGTATGATTCGGCATTCTTTGACAAGCGGTCTAAATTTGTGCATTACACGCCGAAAACGTTAATTATCAA
CAATATTGAATTTGATCACGCGGATATTTTTGACGACTTAAAAGCAATCCAACGCCAATTCCATCACCTAGTACGTACCA
TGCCGAGTGGCGGTTGTATTCTTTCGGCGGCAGCGGATGAGAATGTACAAAATACGCTAAAAATGGGTAGTTACAGCGAG
CTGCAATTTATCGGCGAAGATAAACAATGGTTTGCCAAACCGATTTCTGCCGATTGTTCGCATTTTGAAGTATTCCACAA
AGGTGAAAAAGCCGGCGAAGTCAAATGGAATATTATCGGGGCGCATAATATGCATAATGCACTAATGGCGATTGCCGCGG
CACATCACGCCGGCGTATCGGTAGCAGGTGCTTGCGATGCGCTCGGTTCATTCATTAATGCAAACCGCCGTTTAGAAGTA
AAAGGCGAAGTGAAAGGTATTACCGTCTATGACGACTTTGCTCACCATCCGACCGCAATTGCCGCAACAATTGACGCTTT
ACGTGGCAAAGTCGGCAAAGAACAACGTATTTTGGCGGTGCTTGAACCCCGTTCCAATACGATGAAAATGGGCGTGCATA
AAGAAGAGATCGCACCGAGTTTAGCCGATGCGAATGCGGTATTCGTTTACCAACCGGACACGATTCCGTGGGCGGTTTCA
GTGATTACCGATAATTTAACCCAACCTGCCAAATGGTCGGCGGATTTAGATGAATTGGTCAAAATGATTGTGGAAGAAGC
GAAACCGACGGACCATATTTTAGTGATGAGTAACGGTGCGTTTGGTGGAATTCATCAGAAATTGCTACAAGCATTGAAAG
CTTAA

Upstream 100 bases:

>100_bases
GAATAGTAAGTAATATTATAGAGATAAACCGATGTTACCTCTAATAAGGTACAACAATTTTGTGACATTGATCACATATT
TTTTACATGAAGGTGAATTT

Downstream 100 bases:

>100_bases
CGAGTAGGGCGGATATTCCCGCCCTACAATATCTTAAAGTGGTGTCATTGCAAAAATTTTCGCAAAAATGACCGCTTGTT
TTCCTGTTACTTACTCTTTG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MRQKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFLESHGIDIIPNYDVAQLQPAPDLVVIGNAMSRGNPC
VEYVLDNQLNYTSGPQWLHDHLLKDRWVLAVSGTHGKTTTTGMLAWILDQNNLDAGFLIGGVAGNFGISARAGSSKFFVI
EADEYDSAFFDKRSKFVHYTPKTLIINNIEFDHADIFDDLKAIQRQFHHLVRTMPSGGCILSAAADENVQNTLKMGSYSE
LQFIGEDKQWFAKPISADCSHFEVFHKGEKAGEVKWNIIGAHNMHNALMAIAAAHHAGVSVAGACDALGSFINANRRLEV
KGEVKGITVYDDFAHHPTAIAATIDALRGKVGKEQRILAVLEPRSNTMKMGVHKEEIAPSLADANAVFVYQPDTIPWAVS
VITDNLTQPAKWSADLDELVKMIVEEAKPTDHILVMSNGAFGGIHQKLLQALKA

Sequences:

>Translated_454_residues
MRQKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFLESHGIDIIPNYDVAQLQPAPDLVVIGNAMSRGNPC
VEYVLDNQLNYTSGPQWLHDHLLKDRWVLAVSGTHGKTTTTGMLAWILDQNNLDAGFLIGGVAGNFGISARAGSSKFFVI
EADEYDSAFFDKRSKFVHYTPKTLIINNIEFDHADIFDDLKAIQRQFHHLVRTMPSGGCILSAAADENVQNTLKMGSYSE
LQFIGEDKQWFAKPISADCSHFEVFHKGEKAGEVKWNIIGAHNMHNALMAIAAAHHAGVSVAGACDALGSFINANRRLEV
KGEVKGITVYDDFAHHPTAIAATIDALRGKVGKEQRILAVLEPRSNTMKMGVHKEEIAPSLADANAVFVYQPDTIPWAVS
VITDNLTQPAKWSADLDELVKMIVEEAKPTDHILVMSNGAFGGIHQKLLQALKA
>Mature_454_residues
MRQKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFLESHGIDIIPNYDVAQLQPAPDLVVIGNAMSRGNPC
VEYVLDNQLNYTSGPQWLHDHLLKDRWVLAVSGTHGKTTTTGMLAWILDQNNLDAGFLIGGVAGNFGISARAGSSKFFVI
EADEYDSAFFDKRSKFVHYTPKTLIINNIEFDHADIFDDLKAIQRQFHHLVRTMPSGGCILSAAADENVQNTLKMGSYSE
LQFIGEDKQWFAKPISADCSHFEVFHKGEKAGEVKWNIIGAHNMHNALMAIAAAHHAGVSVAGACDALGSFINANRRLEV
KGEVKGITVYDDFAHHPTAIAATIDALRGKVGKEQRILAVLEPRSNTMKMGVHKEEIAPSLADANAVFVYQPDTIPWAVS
VITDNLTQPAKWSADLDELVKMIVEEAKPTDHILVMSNGAFGGIHQKLLQALKA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=447, Percent_Identity=66.2192393736018, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=361, Percent_Identity=30.7479224376731, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49641; Mature: 49641

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFLESHGIDIIPNYDVAQ
CCCCEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEECCCCHHE
LQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLLKDRWVLAVSGTHGKTTT
ECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
TGMLAWILDQNNLDAGFLIGGVAGNFGISARAGSSKFFVIEADEYDSAFFDKRSKFVHYT
CEEEEEEEECCCCCCCEEEEECCCCCCCEECCCCCEEEEEECCCCCCHHHHCCCCEEEEC
PKTLIINNIEFDHADIFDDLKAIQRQFHHLVRTMPSGGCILSAAADENVQNTLKMGSYSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCC
LQFIGEDKQWFAKPISADCSHFEVFHKGEKAGEVKWNIIGAHNMHNALMAIAAAHHAGVS
EEEECCCHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCE
VAGACDALGSFINANRRLEVKGEVKGITVYDDFAHHPTAIAATIDALRGKVGKEQRILAV
EHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEE
LEPRSNTMKMGVHKEEIAPSLADANAVFVYQPDTIPWAVSVITDNLTQPAKWSADLDELV
ECCCCCCEEECCCHHHHCCHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHH
KMIVEEAKPTDHILVMSNGAFGGIHQKLLQALKA
HHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRQKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFLESHGIDIIPNYDVAQ
CCCCEEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEECCCCHHE
LQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLLKDRWVLAVSGTHGKTTT
ECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCC
TGMLAWILDQNNLDAGFLIGGVAGNFGISARAGSSKFFVIEADEYDSAFFDKRSKFVHYT
CEEEEEEEECCCCCCCEEEEECCCCCCCEECCCCCEEEEEECCCCCCHHHHCCCCEEEEC
PKTLIINNIEFDHADIFDDLKAIQRQFHHLVRTMPSGGCILSAAADENVQNTLKMGSYSE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCC
LQFIGEDKQWFAKPISADCSHFEVFHKGEKAGEVKWNIIGAHNMHNALMAIAAAHHAGVS
EEEECCCHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCE
VAGACDALGSFINANRRLEVKGEVKGITVYDDFAHHPTAIAATIDALRGKVGKEQRILAV
EHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEE
LEPRSNTMKMGVHKEEIAPSLADANAVFVYQPDTIPWAVSVITDNLTQPAKWSADLDELV
ECCCCCCEEECCCHHHHCCHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHH
KMIVEEAKPTDHILVMSNGAFGGIHQKLLQALKA
HHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]