Click here to switch to the map view.

The map label for this gene is napB [H]

Identifier: 126208890

GI number: 126208890

Start: 1631633

End: 1632055

Strand: Reverse

Name: napB [H]

Synonym: APL_1426

Alternate gene names: 126208890

Gene position: 1632055-1631633 (Counterclockwise)

Preceding gene: 126208891

Following gene: 126208889

Centisome position: 71.76

GC content: 42.79

Gene sequence:

>423_bases
ATGAGAAAATATCTCGCTCTTATCCTTACTGCAATTACAGGCTTTGCAGTAGCGGAAACGCAAAATTTATCGTTGGATAA
ATTACCGGAAAATGTGGCGCCGGCTTATACCAACCCGCATAAAGATATCGGTAACATTCCGGTGACTTTCCCTCATCAAC
CGCCGCTTGTTCCGCATAGTATTCGTGGTTTACAAGTAACTAAAAATACCAACCAATGTTTAGGTTGCCATGCGCCGGAA
GTATCAAGCACGACGGGTGCTCCGCGTGTGCCTAAAACGCATTTTATGGATCGAGACGGTAAATTGACGGAAGGAACCTC
TCCGCGTCGTTACTTCTGTTTACAATGCCACGTACAACAAACGGATGTAAATCCGATTATTCAAAATAAATTTGAAACGA
TTCGTCAAATGCAAGGTAAATAA

Upstream 100 bases:

>100_bases
ACGTTGTATTGACGTTTGTCCGGAAAATGTTTTTGCCTTCGGAACACGTTTTCAAGGCAGTATTGAAGTAAAAAATGTAT
AAATCATGGAGTGAATCAAA

Downstream 100 bases:

>100_bases
GGAGCGACATAATGATTAAGAAATTTTGGAACTGGTTCCGTACACCGAGTAAAATGGCGGCAGGTACGCTTATTCTCATT
TCTGCGATTGGCGGTATTTT

Product: nitrate reductase cytochrome c-type subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MRKYLALILTAITGFAVAETQNLSLDKLPENVAPAYTNPHKDIGNIPVTFPHQPPLVPHSIRGLQVTKNTNQCLGCHAPE
VSSTTGAPRVPKTHFMDRDGKLTEGTSPRRYFCLQCHVQQTDVNPIIQNKFETIRQMQGK

Sequences:

>Translated_140_residues
MRKYLALILTAITGFAVAETQNLSLDKLPENVAPAYTNPHKDIGNIPVTFPHQPPLVPHSIRGLQVTKNTNQCLGCHAPE
VSSTTGAPRVPKTHFMDRDGKLTEGTSPRRYFCLQCHVQQTDVNPIIQNKFETIRQMQGK
>Mature_140_residues
MRKYLALILTAITGFAVAETQNLSLDKLPENVAPAYTNPHKDIGNIPVTFPHQPPLVPHSIRGLQVTKNTNQCLGCHAPE
VSSTTGAPRVPKTHFMDRDGKLTEGTSPRRYFCLQCHVQQTDVNPIIQNKFETIRQMQGK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=131, Percent_Identity=43.5114503816794, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15587; Mature: 15587

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYLALILTAITGFAVAETQNLSLDKLPENVAPAYTNPHKDIGNIPVTFPHQPPLVPHS
CHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC
IRGLQVTKNTNQCLGCHAPEVSSTTGAPRVPKTHFMDRDGKLTEGTSPRRYFCLQCHVQQ
CCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEC
TDVNPIIQNKFETIRQMQGK
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKYLALILTAITGFAVAETQNLSLDKLPENVAPAYTNPHKDIGNIPVTFPHQPPLVPHS
CHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC
IRGLQVTKNTNQCLGCHAPEVSSTTGAPRVPKTHFMDRDGKLTEGTSPRRYFCLQCHVQQ
CCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEC
TDVNPIIQNKFETIRQMQGK
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 11389694; 11223519 [H]