Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 126208847

GI number: 126208847

Start: 1584180

End: 1584932

Strand: Reverse

Name: trmB

Synonym: APL_1383

Alternate gene names: 126208847

Gene position: 1584932-1584180 (Counterclockwise)

Preceding gene: 126208848

Following gene: 126208846

Centisome position: 69.68

GC content: 42.76

Gene sequence:

>753_bases
ATGTCAGAAAAACAAACTTTTGCAGATAAAAAACGTAAAACGGTAGAACAAGCCGAATTCACCGAAGACGGTCGCTATCT
TCGTAAAGTACGTAGTTTCGTATTACGTACCGGACGTTTGAGCGATTACCAACGTGATATGATGAATAATAACTGGGCGA
ATCTCGGTTTGGATTATCAAAACACGCCGTTTAATTTTGAGCAGATCTTCGGTAACAACAATCCGGTAGTGTTAGAAATC
GGCTTTGGTATGGGGCGTTCGTTAGTTGAAATGGCGAAGCAAAATCCGGATCGTAACTACATCGGGATTGAAGTACATAC
ACCGGGTGTCGGCGCTTGTATCGCTTATGCATTGGAAAAAGGCGTAAAAAACTTACGTGTAATTTGCCATGACGCAACGG
AAATTTTACGTGATGCGATTGCTGACGGTTCACTTGGCGGTTTACAACTTTATTTCCCGGATCCGTGGCAAAAAGCGAAG
CACCATAAACGCCGGATCGTACAGCCGGAATTTATCACTCGAGTGCTGACTAAATTAGGCGATAATGGTTTTATTCATTT
TGCAACAGACTGGGAAAACTATGCAGAACATATGTTGGAAGTACTACGTCAATTCGACAAAGAGTTACGCAATACCTCGG
CAACCAATGATTTTATTCCACGCCCGGATTTCCGTCCTTTAACCAAGTTTGAAGAACGTGCTCATCGCCTTGGACACGGT
GTGTGGGACTTATATTTCGTTAAACAAGCATAA

Upstream 100 bases:

>100_bases
TTGAAGCCTTTGGCGGCGGCTGGACTTGGGGTTCCGCTCTTGTTCGTTTTTAACCAATCTCGTTTCTAACAAGCGGTCAA
AAAAGATAGAATTTTTACAA

Downstream 100 bases:

>100_bases
TTGGTTTAACGATTGATTTTAACTATCCATTCTCATTAAATAAGGGGAGATACAAATGGCTATTCAACGTAACGCGCGTC
AGCGTAAAAAAATGCACTTA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQRDMMNNNWANLGLDYQNTPFNFEQIFGNNNPVVLEI
GFGMGRSLVEMAKQNPDRNYIGIEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDAIADGSLGGLQLYFPDPWQKAK
HHKRRIVQPEFITRVLTKLGDNGFIHFATDWENYAEHMLEVLRQFDKELRNTSATNDFIPRPDFRPLTKFEERAHRLGHG
VWDLYFVKQA

Sequences:

>Translated_250_residues
MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQRDMMNNNWANLGLDYQNTPFNFEQIFGNNNPVVLEI
GFGMGRSLVEMAKQNPDRNYIGIEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDAIADGSLGGLQLYFPDPWQKAK
HHKRRIVQPEFITRVLTKLGDNGFIHFATDWENYAEHMLEVLRQFDKELRNTSATNDFIPRPDFRPLTKFEERAHRLGHG
VWDLYFVKQA
>Mature_249_residues
SEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQRDMMNNNWANLGLDYQNTPFNFEQIFGNNNPVVLEIG
FGMGRSLVEMAKQNPDRNYIGIEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDAIADGSLGGLQLYFPDPWQKAKH
HKRRIVQPEFITRVLTKLGDNGFIHFATDWENYAEHMLEVLRQFDKELRNTSATNDFIPRPDFRPLTKFEERAHRLGHGV
WDLYFVKQA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=235, Percent_Identity=55.3191489361702, Blast_Score=277, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_ACTP2 (A3N231)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054072.1
- ProteinModelPortal:   A3N231
- SMR:   A3N231
- STRING:   A3N231
- GeneID:   4850187
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1383
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   NIRLIRG
- PhylomeDB:   A3N231
- ProtClustDB:   PRK00121
- BioCyc:   APLE416269:APL_1383-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29025; Mature: 28894

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: BINDING 79-79 BINDING 104-104 BINDING 131-131 BINDING 154-154 BINDING 158-158 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQRDMMNNNWANLGLDYQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCC
NTPFNFEQIFGNNNPVVLEIGFGMGRSLVEMAKQNPDRNYIGIEVHTPGVGACIAYALEK
CCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
GVKNLRVICHDATEILRDAIADGSLGGLQLYFPDPWQKAKHHKRRIVQPEFITRVLTKLG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHC
DNGFIHFATDWENYAEHMLEVLRQFDKELRNTSATNDFIPRPDFRPLTKFEERAHRLGHG
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
VWDLYFVKQA
CEEEEEEECC
>Mature Secondary Structure 
SEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQRDMMNNNWANLGLDYQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCC
NTPFNFEQIFGNNNPVVLEIGFGMGRSLVEMAKQNPDRNYIGIEVHTPGVGACIAYALEK
CCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
GVKNLRVICHDATEILRDAIADGSLGGLQLYFPDPWQKAKHHKRRIVQPEFITRVLTKLG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHC
DNGFIHFATDWENYAEHMLEVLRQFDKELRNTSATNDFIPRPDFRPLTKFEERAHRLGHG
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
VWDLYFVKQA
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA