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The map label for this gene is hpaC [H]

Identifier: 126208813

GI number: 126208813

Start: 1548298

End: 1548810

Strand: Direct

Name: hpaC [H]

Synonym: APL_1349

Alternate gene names: 126208813

Gene position: 1548298-1548810 (Clockwise)

Preceding gene: 126208812

Following gene: 126208814

Centisome position: 68.07

GC content: 45.03

Gene sequence:

>513_bases
ATGTCTTGTATTCCGAAACCTCAAGAGGTACCTGAATTTTCACAACAATTTCGTAATGCGATGGCGCATTTATCCGCTGC
GGTAAGTATTGTGACCACAAACGGGTCTGCCGGCAAAATCGGTATTACCGTATCATCGGTTTGCTCGGTGACCGATACGC
CGCCGACCTTGCTTTTCTGTATCAATCAAAGCAGTTCCGTTCACGATATCATTAAGCAAAACGGTAAAGTGTGCATCAAC
GTATTAAGCCACGAACAAGAAGAAATGGCGAAGCATTTTGCTTGTATGATGTGCAGCAGTATGGAAGAACGTTTCGCTTG
GGATATTTGGGACGAAGGCTTTGACGGACAACCGGTATTACGCGGTTCGATTTCTGCCTTACAAGGCGATATTGTCGATA
CGCATTCGGTGGGTACGCATACGATTTTTATGGTGGAACTGAAACATATCGATGTAACGCCGAATACCGGCTTAGTCTAT
TTCGGTCGTCAATTTAAGGGCATAGCGATCTAA

Upstream 100 bases:

>100_bases
AGATGAAATGGATGACGTATTCGCTCGTTTCTTGATTTCTCACGATGCGGATAACCGAGAATATCACGTGATCTGGAAGG
AATAATTTAAAAGGAGCTTT

Downstream 100 bases:

>100_bases
TAAATCTTAGGTATTTTTGATATAACCGACTATTTTAGTCGGTTTTTTATTGCCTAGAAAAATCAGAAGAGTAAAATTTA
TCATCATTATTTTATTCGTT

Product: 4-hydroxyphenylacetic acid hydroxylase putative coupling protein

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-monooxygenase small component; Flavin:NADH reductase [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MSCIPKPQEVPEFSQQFRNAMAHLSAAVSIVTTNGSAGKIGITVSSVCSVTDTPPTLLFCINQSSSVHDIIKQNGKVCIN
VLSHEQEEMAKHFACMMCSSMEERFAWDIWDEGFDGQPVLRGSISALQGDIVDTHSVGTHTIFMVELKHIDVTPNTGLVY
FGRQFKGIAI

Sequences:

>Translated_170_residues
MSCIPKPQEVPEFSQQFRNAMAHLSAAVSIVTTNGSAGKIGITVSSVCSVTDTPPTLLFCINQSSSVHDIIKQNGKVCIN
VLSHEQEEMAKHFACMMCSSMEERFAWDIWDEGFDGQPVLRGSISALQGDIVDTHSVGTHTIFMVELKHIDVTPNTGLVY
FGRQFKGIAI
>Mature_169_residues
SCIPKPQEVPEFSQQFRNAMAHLSAAVSIVTTNGSAGKIGITVSSVCSVTDTPPTLLFCINQSSSVHDIIKQNGKVCINV
LSHEQEEMAKHFACMMCSSMEERFAWDIWDEGFDGQPVLRGSISALQGDIVDTHSVGTHTIFMVELKHIDVTPNTGLVYF
GRQFKGIAI

Specific function: Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large hpaB component or to other electron acceptors such as cytochrome c and Fe(3+) ion [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=42.9487179487179, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR011982
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.14.13.3

Molecular weight: Translated: 18575; Mature: 18444

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCIPKPQEVPEFSQQFRNAMAHLSAAVSIVTTNGSAGKIGITVSSVCSVTDTPPTLLFC
CCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEHHHEECCCCCCCEEEEE
INQSSSVHDIIKQNGKVCINVLSHEQEEMAKHFACMMCSSMEERFAWDIWDEGFDGQPVL
ECCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCH
RGSISALQGDIVDTHSVGTHTIFMVELKHIDVTPNTGLVYFGRQFKGIAI
HCCHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECCEECCCCC
>Mature Secondary Structure 
SCIPKPQEVPEFSQQFRNAMAHLSAAVSIVTTNGSAGKIGITVSSVCSVTDTPPTLLFC
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEHHHEECCCCCCCEEEEE
INQSSSVHDIIKQNGKVCINVLSHEQEEMAKHFACMMCSSMEERFAWDIWDEGFDGQPVL
ECCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCH
RGSISALQGDIVDTHSVGTHTIFMVELKHIDVTPNTGLVYFGRQFKGIAI
HCCHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10633095 [H]